ID   BRK_POLOC               Reviewed;           9 AA.
AC   C0HLT7;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   07-APR-2021, sequence version 1.
DT   25-MAY-2022, entry version 5.
DE   RecName: Full=Thr6-bradykinin {ECO:0000303|PubMed:17533426};
DE   AltName: Full=Bradykinin-related peptide {ECO:0000305};
OS   Polybia occidentalis (Paper wasp).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Vespoidea;
OC   Vespidae; Polistinae; Epiponini; Polybia.
OX   NCBI_TaxID=91432;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RC   TISSUE=Venom gland;
RX   PubMed=17533426; DOI=10.1038/sj.bjp.0707275;
RA   Mortari M.R., Cunha A.O., Carolino R.O., Coutinho-Netto J., Tomaz J.C.,
RA   Lopes N.P., Coimbra N.C., Dos Santos W.F.;
RT   "Inhibition of acute nociceptive responses in rats after i.c.v. injection
RT   of Thr6-bradykinin, isolated from the venom of the social wasp, Polybia
RT   occidentalis.";
RL   Br. J. Pharmacol. 151:860-869(2007).
RN   [2]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RA   Genta-Jouve G.;
RL   Submitted (OCT-2020) to UniProtKB.
CC   -!- FUNCTION: When injected into the rat central nervous system, exhibits
CC       antinociceptive effects such as increasing hot plate and tail flick
CC       withdrawal latencies in a dose- and time-dependent fashion
CC       (PubMed:17533426). Likely to act on the bradykinin receptor B2 (BDKRB2)
CC       (PubMed:17533426). The greatest antinociceptive effects occurs 30
CC       minutes after injection in hot plate (ED(50)=6.82 nM) and tail flick
CC       tests (ED(50)=2.94 nM) (PubMed:17533426).
CC       {ECO:0000269|PubMed:17533426}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17533426,
CC       ECO:0000269|Ref.2}.
CC   -!- TISSUE SPECIFICITY: Expressed in the venom reservoir.
CC       {ECO:0000305|PubMed:17533426, ECO:0000305|Ref.2}.
CC   -!- MASS SPECTROMETRY: Mass=1074.8; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:17533426};
CC   -!- MASS SPECTROMETRY: Mass=1073; Mass_error=2; Method=Electrospray;
CC       Evidence={ECO:0000269|Ref.2};
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0031713; F:B2 bradykinin receptor binding; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0044514; P:envenomation resulting in positive regulation of G protein-coupled receptor activity in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Bradykinin receptor impairing toxin; Direct protein sequencing;
KW   G-protein coupled receptor impairing toxin; Neurotoxin; Secreted; Toxin.
FT   PEPTIDE         1..9
FT                   /note="Thr6-bradykinin"
FT                   /evidence="ECO:0000269|PubMed:17533426"
FT                   /id="PRO_0000452238"
SQ   SEQUENCE   9 AA;  1074 MW;  3393D771A9C86777 CRC64;
     RPPGFTPFR