ID   BRK_TRICX               Reviewed;           9 AA.
AC   P0DM76;
DT   13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2013, sequence version 1.
DT   03-AUG-2022, entry version 13.
DE   RecName: Full=Bradykinin;
OS   Trichonephila clavipes (Golden silk orbweaver) (Nephila clavipes).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Araneoidea; Nephilidae; Trichonephila.
OX   NCBI_TaxID=2585209;
RN   [1]
RP   PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY, SYNTHESIS, FUNCTION,
RP   BIOASSAY, AND TOXIC DOSE.
RX   PubMed=16202476; DOI=10.1016/j.peptides.2005.08.011;
RA   Volsi E.C., Mendes M.A., Marques M.R., dos Santos L.D., Santos K.S.,
RA   de Souza B.M., Babieri E.F., Palma M.S.;
RT   "Multiple bradykinin-related peptides from the capture web of the spider
RT   Nephila clavipes (Araneae, Tetragnatidae).";
RL   Peptides 27:690-697(2006).
CC   -!- FUNCTION: Causes constriction on isolated rat ileum preparations and
CC       relaxation on rat duodenum muscle preparations at amounts higher than
CC       bradykinin. Targets bradykinin receptor B2 (BDKRB2). Has insecticidal
CC       properties. May be related to the predation of insects by the spider
CC       webs. {ECO:0000269|PubMed:16202476}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TOXIC DOSE: LD(50) is 54 pmoles/mg when injected into honeybees. Dose
CC       that causes muscle constriction (ED(50)) is 0.3 uM. Dose that causes
CC       muscle relaxation (ED(50)) is 0.2 uM. {ECO:0000269|PubMed:16202476}.
CC   -!- MISCELLANEOUS: Has been extracted from the spider web.
CC       {ECO:0000305|PubMed:16202476}.
CC   -!- SIMILARITY: Belongs to the bradykinin-related peptide family.
CC       {ECO:0000305}.
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DR   SMR; P0DM76; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; G-protein coupled receptor impairing toxin;
KW   Secreted; Toxin; Vasoactive; Vasodilator.
FT   PEPTIDE         1..9
FT                   /note="Bradykinin"
FT                   /id="PRO_0000424408"
SQ   SEQUENCE   9 AA;  1060 MW;  3393D775B9C86777 CRC64;
     RPPGFSPFR