ID   BRL05_BRELC             Reviewed;          97 AA.
AC   P0CU86;
DT   18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT   18-SEP-2019, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=Secreted RxLR effector protein BLR05 {ECO:0000303|PubMed:23883357};
DE   Flags: Precursor;
GN   Name=BLR05 {ECO:0000303|PubMed:23883357};
OS   Bremia lactucae (Lettuce downy mildew).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Bremia.
OX   NCBI_TaxID=4779;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND DOMAIN.
RX   PubMed=23883357; DOI=10.1094/mpmi-05-13-0142-r;
RA   Stassen J.H., den Boer E., Vergeer P.W., Andel A., Ellendorff U.,
RA   Pelgrom K., Pel M., Schut J., Zonneveld O., Jeuken M.J.,
RA   Van den Ackerveken G.;
RT   "Specific in planta recognition of two GKLR proteins of the downy mildew
RT   Bremia lactucae revealed in a large effector screen in lettuce.";
RL   Mol. Plant Microbe Interact. 26:1259-1270(2013).
RN   [2]
RP   INTERACTION WITH NAC069, SUBCELLULAR LOCATION, AND FUNCTION.
RX   PubMed=31077456; DOI=10.1111/tpj.14383;
RA   Meisrimler C.N., Pelgrom A.J.E., Oud B., Out S., Van den Ackerveken G.;
RT   "Multiple downy mildew effectors target the stress-related NAC
RT   transcription factor LsNAC069 in lettuce.";
RL   Plant J. 99:1098-1115(2019).
CC   -!- FUNCTION: Secreted effector that inhibits stress-induced relocalization
CC       of the transcription factor NAC069 to the nucleus, thus affecting its
CC       broad role in abiotic and biotic stress responses.
CC       {ECO:0000269|PubMed:31077456}.
CC   -!- SUBUNIT: Interacts with host transcription factor NAC069.
CC       {ECO:0000269|PubMed:31077456}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31077456}. Host
CC       endoplasmic reticulum membrane {ECO:0000269|PubMed:31077456}; Single-
CC       pass type I membrane protein {ECO:0000255}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:23883357}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P0CU86; -.
DR   VEuPathDB; FungiDB:CCR75_006001; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Host endoplasmic reticulum; Host membrane; Membrane; Secreted; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..97
FT                   /note="Secreted RxLR effector protein BLR05"
FT                   /id="PRO_0000447898"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           32..60
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:23883357"
SQ   SEQUENCE   97 AA;  10656 MW;  89A24DF0A381970D CRC64;
     MGPQHLLALV VVSILVAAGN AYYEIDDDSV TRALRPSVIA DQEHAVHAIP ATNFISKDED
     HSKNEKKEIE IIRIAIFSLL VVGVFAIMAL RCLPFCL