ID   BRL08_BRELC             Reviewed;         135 AA.
AC   P0CU87;
DT   18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT   18-SEP-2019, sequence version 1.
DT   25-MAY-2022, entry version 8.
DE   RecName: Full=Secreted RxLR effector protein BLR08 {ECO:0000303|PubMed:23883357};
DE   Flags: Precursor;
GN   Name=BLR08 {ECO:0000303|PubMed:23883357};
OS   Bremia lactucae (Lettuce downy mildew).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Bremia.
OX   NCBI_TaxID=4779;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND DOMAIN.
RX   PubMed=23883357; DOI=10.1094/mpmi-05-13-0142-r;
RA   Stassen J.H., den Boer E., Vergeer P.W., Andel A., Ellendorff U.,
RA   Pelgrom K., Pel M., Schut J., Zonneveld O., Jeuken M.J.,
RA   Van den Ackerveken G.;
RT   "Specific in planta recognition of two GKLR proteins of the downy mildew
RT   Bremia lactucae revealed in a large effector screen in lettuce.";
RL   Mol. Plant Microbe Interact. 26:1259-1270(2013).
RN   [2]
RP   INTERACTION WITH NAC069, AND FUNCTION.
RX   PubMed=31077456; DOI=10.1111/tpj.14383;
RA   Meisrimler C.N., Pelgrom A.J.E., Oud B., Out S., Van den Ackerveken G.;
RT   "Multiple downy mildew effectors target the stress-related NAC
RT   transcription factor LsNAC069 in lettuce.";
RL   Plant J. 99:1098-1115(2019).
CC   -!- FUNCTION: Secreted effector that inhibits stress-induced relocalization
CC       of the transcription factor NAC069 to the nucleus, thus affecting its
CC       broad role in abiotic and biotic stress responses.
CC       {ECO:0000269|PubMed:31077456}.
CC   -!- SUBUNIT: Interacts with host transcription factor NAC069.
CC       {ECO:0000269|PubMed:31077456}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31077456}. Host
CC       membrane {ECO:0000269|PubMed:31077456}; Single-pass type I membrane
CC       protein {ECO:0000255}. Note=Localizes to unidentified ring-like
CC       structures of varying size within the host cell.
CC       {ECO:0000269|PubMed:31077456}.
CC   -!- DOMAIN: Has the canonical translocation RxLR motif, but lacks the
CC       canonical EER motif, which characterizes most oomycete effectors
CC       identified so far. {ECO:0000305|PubMed:23883357}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   AlphaFoldDB; P0CU87; -.
DR   VEuPathDB; FungiDB:CCR75_004770; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Glycoprotein; Host membrane; Membrane; Secreted; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..135
FT                   /note="Secreted RxLR effector protein BLR08"
FT                   /id="PRO_0000447899"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          56..97
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           38..41
FT                   /note="RxLR"
FT                   /evidence="ECO:0000305|PubMed:23883357"
FT   CARBOHYD        84
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   135 AA;  14056 MW;  D1402344D39B6966 CRC64;
     MAEFRFRPTT LLFLIVALIF RCAIDPVSAN ADLTPSPRLL RDLVQFDATQ TVAHTLSNSP
     SASSSSAEAT VHPAASAEAS AISNHTTDNA ASAESSAESK HELPSIMSFI GPAAAGVLAI
     VLIGAVIAFK YRSSK