BRL1_YEAST
ID BRL1_YEAST Reviewed; 471 AA.
AC P38770; D3DKY3;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Nucleus export protein BRL1;
DE AltName: Full=BRR6-like protein 1;
GN Name=BRL1; OrderedLocusNames=YHR036W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH BRR6.
RX PubMed=15882446; DOI=10.1111/j.1600-0854.2005.00295.x;
RA Saitoh Y.-H., Ogawa K., Nishimoto T.;
RT "Brl1p - a novel nuclear envelope protein required for nuclear transport.";
RL Traffic 6:502-517(2005).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Involved in mRNA and protein export from nucleus.
CC {ECO:0000269|PubMed:15882446}.
CC -!- SUBUNIT: Interacts with BRR6. {ECO:0000269|PubMed:15882446}.
CC -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000269|PubMed:15882446};
CC Multi-pass membrane protein {ECO:0000269|PubMed:15882446}.
CC -!- MISCELLANEOUS: Present with 1280 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the BRL1/BRR6 family. {ECO:0000305}.
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DR EMBL; U00062; AAB68908.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06727.1; -; Genomic_DNA.
DR PIR; S46739; S46739.
DR RefSeq; NP_011901.1; NM_001179166.1.
DR AlphaFoldDB; P38770; -.
DR SMR; P38770; -.
DR BioGRID; 36467; 830.
DR DIP; DIP-5580N; -.
DR IntAct; P38770; 12.
DR MINT; P38770; -.
DR STRING; 4932.YHR036W; -.
DR TCDB; 9.A.34.1.1; the nuclear pore complex biogenesis (npc-b) family.
DR iPTMnet; P38770; -.
DR MaxQB; P38770; -.
DR PaxDb; P38770; -.
DR PRIDE; P38770; -.
DR EnsemblFungi; YHR036W_mRNA; YHR036W; YHR036W.
DR GeneID; 856431; -.
DR KEGG; sce:YHR036W; -.
DR SGD; S000001078; BRL1.
DR VEuPathDB; FungiDB:YHR036W; -.
DR eggNOG; KOG4503; Eukaryota.
DR HOGENOM; CLU_031411_1_0_1; -.
DR InParanoid; P38770; -.
DR OMA; ETHSSMT; -.
DR BioCyc; YEAST:G3O-31096-MON; -.
DR PRO; PR:P38770; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38770; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IDA:SGD.
DR GO; GO:0005635; C:nuclear envelope; IDA:SGD.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0055088; P:lipid homeostasis; IMP:SGD.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0006998; P:nuclear envelope organization; IMP:SGD.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR040202; Brl1/Brr6.
DR InterPro; IPR018767; Brl1/Brr6_dom.
DR PANTHER; PTHR28136; PTHR28136; 1.
DR Pfam; PF10104; Brr6_like_C_C; 1.
DR SMART; SM01042; Brr6_like_C_C; 1.
PE 1: Evidence at protein level;
KW Membrane; mRNA transport; Nucleus; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..471
FT /note="Nucleus export protein BRL1"
FT /id="PRO_0000202892"
FT TOPO_DOM 1..299
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 321..407
FT /note="Perinuclear space"
FT /evidence="ECO:0000255"
FT TRANSMEM 408..428
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 429..471
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 66..135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 167..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 444..471
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 73..91
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..130
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 173..187
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 444..465
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 471 AA; 53385 MW; 732209EC74DE3463 CRC64;
MESFENLSIR DSFTSGMEHV DEELGGLSDL SISKQGPTLS PQLINRFMPH FPSSPSPLRN
TLDFSAAKAD EEEDDRMEID EVDDTSFEEE YNNEPIETHT EATENAVVEE IEATPEERQK
QEKNESQDQS VEEVENIVSP HRSTVIKALL SPTDLGVAAA TKVEGVVPLP PSANQDDNES
SNNNAEGEDI IRNEEVEDEI KSSLGNHKSS QYANAFDSEI IKRELRSRSK YQPIQVSFNT
HNYFYSDKDG IKTYSLTKPN HNKIDEFYDQ NEAFKLPKPW SPNSHPASRA SYALMSYLQL
FLNAITTVVI FSFILSFIIA LQKDLKSTWE QRKHELQYES RICQEQYLTN RCNQTPGLPA
LGEQCAIWKQ CMDRNNDIFF RARSTLSAKL FGDIINSFID PLNWKTLFVI FCGVITWCFS
SNFLLGFVRA KSYYGNGIKT YPLPSSPKSP TSEETHSSMT ASGEDSHLLK Q
