BRLA_EMENI
ID BRLA_EMENI Reviewed; 432 AA.
AC P10069; C8VUE5; Q5BEQ7;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 25-MAY-2022, entry version 132.
DE RecName: Full=C2H2 type master regulator of conidiophore development brlA {ECO:0000305};
DE AltName: Full=Bristle A protein {ECO:0000303|PubMed:5366214};
GN Name=brlA {ECO:0000303|PubMed:3293800}; ORFNames=AN0973;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=3293800; DOI=10.1016/0092-8674(88)90198-5;
RA Adams T.H., Boylan M.T., Timberlake W.E.;
RT "brlA is necessary and sufficient to direct conidiophore development in
RT Aspergillus nidulans.";
RL Cell 54:353-362(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9290249; DOI=10.1006/fgbi.1997.0982;
RA Kupfer D.M., Reece C.A., Clifton S.W., Roe B.A., Prade R.A.;
RT "Multicellular ascomycetous fungal genomes contain more than 8000 genes.";
RL Fungal Genet. Biol. 21:364-372(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-37, AND FUNCTION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=8508769; DOI=10.1002/j.1460-2075.1993.tb05898.x;
RA Prade R.A., Timberlake W.E.;
RT "The Aspergillus nidulans brlA regulatory locus consists of overlapping
RT transcription units that are individually required for conidiophore
RT development.";
RL EMBO J. 12:2439-2447(1993).
RN [6]
RP FUNCTION.
RX PubMed=5366214; DOI=10.1093/genetics/63.2.317;
RA Clutterbuck A.J.;
RT "A mutational analysis of conidial development in Aspergillus nidulans.";
RL Genetics 63:317-327(1969).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION.
RX PubMed=2823119; DOI=10.1128/mcb.7.9.3113-3118.1987;
RA Boylan M.T., Mirabito P.M., Willett C.E., Zimmerman C.R., Timberlake W.E.;
RT "Isolation and physical characterization of three essential conidiation
RT genes from Aspergillus nidulans.";
RL Mol. Cell. Biol. 7:3113-3118(1987).
RN [8]
RP FUNCTION, AND INDUCTION.
RX PubMed=2655931; DOI=10.1016/0092-8674(89)90800-3;
RA Mirabito P.M., Adams T.H., Timberlake W.E.;
RT "Interactions of three sequentially expressed genes control temporal and
RT spatial specificity in Aspergillus development.";
RL Cell 57:859-868(1989).
RN [9]
RP FUNCTION, MUTAGENESIS OF CYS-327 AND CYS-357, AND DOMAIN.
RX PubMed=2108321; DOI=10.1128/mcb.10.4.1815-1817.1990;
RA Adams T.H., Deising H., Timberlake W.E.;
RT "brlA requires both zinc fingers to induce development.";
RL Mol. Cell. Biol. 10:1815-1817(1990).
RN [10]
RP FUNCTION.
RX PubMed=2196567; DOI=10.1073/pnas.87.14.5405;
RA Adams T.H., Timberlake W.E.;
RT "Developmental repression of growth and gene expression in Aspergillus.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:5405-5409(1990).
RN [11]
RP FUNCTION.
RX PubMed=1901560; DOI=10.1016/0378-1119(91)90105-k;
RA Birse C.E., Clutterbuck A.J.;
RT "Isolation and developmentally regulated expression of an Aspergillus
RT nidulans phenol oxidase-encoding gene, ivoB.";
RL Gene 98:69-76(1991).
RN [12]
RP FUNCTION.
RX PubMed=2065971; DOI=10.1101/gad.5.7.1161;
RA Stringer M.A., Dean R.A., Sewall T.C., Timberlake W.E.;
RT "Rodletless, a new Aspergillus developmental mutant induced by directed
RT gene inactivation.";
RL Genes Dev. 5:1161-1171(1991).
RN [13]
RP INDUCTION.
RX PubMed=1508186; DOI=10.1128/mcb.12.9.3827-3833.1992;
RA Adams T.H., Hide W.A., Yager L.N., Lee B.N.;
RT "Isolation of a gene required for programmed initiation of development by
RT Aspergillus nidulans.";
RL Mol. Cell. Biol. 12:3827-3833(1992).
RN [14]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=8417986; DOI=10.1093/genetics/133.1.29;
RA Chang Y.C., Timberlake W.E.;
RT "Identification of Aspergillus brlA response elements (BREs) by genetic
RT selection in yeast.";
RL Genetics 133:29-38(1993).
RN [15]
RP FUNCTION.
RX PubMed=7704830; DOI=10.1139/m94-164;
RA Sewall T.C.;
RT "Cellular effects of misscheduled brlA, abaA, and wetA expression in
RT Aspergillus nidulans.";
RL Can. J. Microbiol. 40:1035-1042(1994).
RN [16]
RP INDUCTION.
RX PubMed=24587098; DOI=10.1371/journal.pone.0089883;
RA Park H.S., Nam T.Y., Han K.H., Kim S.C., Yu J.H.;
RT "VelC positively controls sexual development in Aspergillus nidulans.";
RL PLoS ONE 9:E89883-E89883(2014).
CC -!- FUNCTION: BrlA, abaA and wetA are pivotal regulators of conidiophore
CC development and conidium maturation (PubMed:8508769, PubMed:5366214,
CC PubMed:2823119, PubMed:2655931, PubMed:2196567,PubMed:7704830). They
CC act individually and together to regulate their own expression and that
CC of numerous other sporulation-specific genes (PubMed:2823119,
CC PubMed:2655931, PubMed:2108321). Binds promoters of target genes at
CC brlA response elements (BREs) containing the conserved sequence 5'-
CC (C/A)(A/G)AGGG(G/A)-3' (PubMed:8417986). Controls the expression of the
CC conidiophore-specific phenol oxidase ivoB (PubMed:1901560). Controls
CC the expression of the hydrophobin rodA (PubMed:2065971). Mediates the
CC developmental switch from the indeterminate, apical growth pattern of
CC vegetative cells to the budding growth pattern of conidiophores
CC (PubMed:3293800). Expression of brlA leads to activation of abaA, wetA
CC and stuA, cessation of vegetative growth, cellular vacuolization and
CC spore formation. {ECO:0000269|PubMed:1901560,
CC ECO:0000269|PubMed:2065971, ECO:0000269|PubMed:2108321,
CC ECO:0000269|PubMed:2196567, ECO:0000269|PubMed:2655931,
CC ECO:0000269|PubMed:2823119, ECO:0000269|PubMed:3293800,
CC ECO:0000269|PubMed:5366214, ECO:0000269|PubMed:7704830,
CC ECO:0000269|PubMed:8417986, ECO:0000269|PubMed:8508769}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- INDUCTION: Expression is induced during conidiation, after conidiophore
CC stalks had formed and had begun to vesiculate (PubMed:2823119).
CC Positively regulated by abaA (PubMed:2655931). Negatively regulated by
CC velC (PubMed:24587098). Expression is also controlled by acoD
CC (PubMed:1508186). {ECO:0000269|PubMed:1508186,
CC ECO:0000269|PubMed:24587098, ECO:0000269|PubMed:2655931,
CC ECO:0000269|PubMed:2823119}.
CC -!- DOMAIN: Both zinc fingers are required to induce development
CC (PubMed:2108321). {ECO:0000269|PubMed:2108321}.
CC -!- DISRUPTION PHENOTYPE: Prevents the formation of normal conidiophores
CC (PubMed:2823119). {ECO:0000269|PubMed:2823119}.
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DR EMBL; M20631; AAA33300.1; -; Genomic_DNA.
DR EMBL; AC000133; AAB48671.1; -; Genomic_DNA.
DR EMBL; AACD01000014; EAA66002.1; -; Genomic_DNA.
DR EMBL; S62606; AAB27149.1; -; Genomic_DNA.
DR EMBL; BN001308; CBF88417.1; -; Genomic_DNA.
DR PIR; A28913; A28913.
DR RefSeq; XP_658577.1; XM_653485.1.
DR AlphaFoldDB; P10069; -.
DR SMR; P10069; -.
DR STRING; 162425.CADANIAP00001679; -.
DR EnsemblFungi; CBF88417; CBF88417; ANIA_00973.
DR EnsemblFungi; EAA66002; EAA66002; AN0973.2.
DR GeneID; 2876748; -.
DR KEGG; ani:AN0973.2; -.
DR VEuPathDB; FungiDB:AN0973; -.
DR eggNOG; KOG1721; Eukaryota.
DR HOGENOM; CLU_655506_0_0_1; -.
DR InParanoid; P10069; -.
DR OMA; NPCLSRP; -.
DR OrthoDB; 633411at2759; -.
DR Proteomes; UP000000560; Chromosome VIII.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:AspGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0001896; P:autolysis; IMP:AspGD.
DR GO; GO:0048315; P:conidium formation; IMP:AspGD.
DR GO; GO:0075307; P:positive regulation of conidium formation; IMP:AspGD.
DR GO; GO:1900376; P:regulation of secondary metabolite biosynthetic process; IMP:AspGD.
DR GO; GO:0010913; P:regulation of sterigmatocystin biosynthetic process; IMP:AspGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:AspGD.
DR GO; GO:0043619; P:regulation of transcription from RNA polymerase II promoter in response to oxidative stress; IBA:GO_Central.
DR GO; GO:0042594; P:response to starvation; IEP:AspGD.
DR GO; GO:0000905; P:sporocarp development involved in asexual reproduction; IMP:AspGD.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR GO; GO:0045461; P:sterigmatocystin biosynthetic process; IMP:AspGD.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 2.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Activator; Conidiation; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Sporulation; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..432
FT /note="C2H2 type master regulator of conidiophore
FT development brlA"
FT /id="PRO_0000046869"
FT ZN_FING 320..344
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 350..375
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 22..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 238..260
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 388..432
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..51
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 327
FT /note="C->S: Fails to induce either the asexual
FT reproductive pathway or the expression of development-
FT specific genes."
FT /evidence="ECO:0000269|PubMed:2108321"
FT MUTAGEN 357
FT /note="C->S: Fails to induce either the asexual
FT reproductive pathway or the expression of development-
FT specific genes."
FT /evidence="ECO:0000269|PubMed:2108321"
SQ SEQUENCE 432 AA; 47932 MW; CA93E6A21CD33E25 CRC64;
MRNQSSLSDR LTVEVDCSSL GSNECPSMTS SFSPLESPTP TPTSIYSQGS LESPGWHGAG
SLPNNTYERT PGSASMRSAF RLAGMASTES LGLPYGSMEG QERMPMPDFL SGYDENIEQL
WMPSEAPKSY DHVAQGLAYH QGMHQYPTMA RNTNNNYRHQ AAAYLPESTT NPCLSRSIFH
QPERVPSSMS SSMSMNNMLP WMNLGDSIAP QTIAPSQVGP VTPPPSYTDF PTSLSAFKQH
SPTTPIRSCS LGTGSGADTP LSRLSGGPCE YMDDFQPSPV YRDGFQRPHR VASRKMLRRQ
TSKQNLMLEN LPQVIKQVQF KCKEPGCNGR FKRQEHLKRH MKSHSKEKPH VCWVPGCHRA
FSRSDNLNAH YTKTHSKRGG RNRYVATLDE NSPDYDPEFR GQLTPDGRPI YGSKLDDPIP
GAGDMSLDGW DE
