ID   BRLA_METRA              Reviewed;         368 AA.
AC   A0A0B2XG51;
DT   05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT   04-MAR-2015, sequence version 1.
DT   25-MAY-2022, entry version 21.
DE   RecName: Full=C2H2 type master regulator of conidiophore development BrlA {ECO:0000303|PubMed:29958281};
GN   Name=BrlA {ECO:0000303|PubMed:29958281}; ORFNames=MAA_10599;
OS   Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) (Metarhizium
OS   anisopliae (strain ARSEF 23)).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX   NCBI_TaxID=655844;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ARSEF 23 / ATCC MYA-3075;
RX   PubMed=21253567; DOI=10.1371/journal.pgen.1001264;
RA   Gao Q., Jin K., Ying S.-H., Zhang Y., Xiao G., Shang Y., Duan Z., Hu X.,
RA   Xie X.-Q., Zhou G., Peng G., Luo Z., Huang W., Wang B., Fang W., Wang S.,
RA   Zhong Y., Ma L.-J., St Leger R.J., Zhao G.-P., Pei Y., Feng M.-G., Xia Y.,
RA   Wang C.;
RT   "Genome sequencing and comparative transcriptomics of the model
RT   entomopathogenic fungi Metarhizium anisopliae and M. acridum.";
RL   PLoS Genet. 7:E1001264-E1001264(2011).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ARSEF 23 / ATCC MYA-3075;
RX   PubMed=25368161; DOI=10.1073/pnas.1412662111;
RA   Hu X., Xiao G., Zheng P., Shang Y., Su Y., Zhang X., Liu X., Zhan S.,
RA   St Leger R.J., Wang C.;
RT   "Trajectory and genomic determinants of fungal-pathogen speciation and host
RT   adaptation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 111:16796-16801(2014).
RN   [3]
RP   IDENTIFICATION, DISRUPTION PHENOTYPE, FUNCTION, AND INDUCTION.
RX   PubMed=29958281; DOI=10.1371/journal.pgen.1007472;
RA   Zeng G., Zhang P., Zhang Q., Zhao H., Li Z., Zhang X., Wang C., Yin W.B.,
RA   Fang W.;
RT   "Duplication of a Pks gene cluster and subsequent functional
RT   diversification facilitate environmental adaptation in Metarhizium
RT   species.";
RL   PLoS Genet. 14:E1007472-E1007472(2018).
CC   -!- FUNCTION: BrlA, abaA and wetA are pivotal regulators of conidiophore
CC       development and conidium maturation. They act individually and together
CC       to regulate their own expression and that of numerous other
CC       sporulation-specific genes (PubMed:29958281). BrlA, abaA and wetA act
CC       together to positively regulate the expression of the Pks1 gene cluster
CC       that mediates the biosynthesis of an anthraquinone derivative pigment
CC       that contributes to conidial pigmentation that provides protection from
CC       UV radiation, heat and cold stress (PubMed:29958281).
CC       {ECO:0000269|PubMed:29958281}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- INDUCTION: Expression remains constant during conidiation.
CC       {ECO:0000269|PubMed:29958281}.
CC   -!- DOMAIN: Both zinc fingers are required to induce development.
CC       {ECO:0000250|UniProtKB:P10069}.
CC   -!- DISRUPTION PHENOTYPE: Leads to immature conidiophores without
CC       phialides. {ECO:0000269|PubMed:29958281}.
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DR   EMBL; ADNJ02000001; KHO11655.1; -; Genomic_DNA.
DR   RefSeq; XP_011410619.1; XM_011412317.1.
DR   AlphaFoldDB; A0A0B2XG51; -.
DR   SMR; A0A0B2XG51; -.
DR   EnsemblFungi; KHO11655; KHO11655; MAA_10599.
DR   GeneID; 19264885; -.
DR   KEGG; maj:MAA_10599; -.
DR   HOGENOM; CLU_064755_0_0_1; -.
DR   Proteomes; UP000002498; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0048315; P:conidium formation; IEA:UniProtKB-KW.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   SMART; SM00355; ZnF_C2H2; 2.
DR   SUPFAM; SSF57667; SSF57667; 1.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE   2: Evidence at transcript level;
KW   Activator; Conidiation; DNA-binding; Metal-binding; Nucleus; Repeat;
KW   Sporulation; Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..368
FT                   /note="C2H2 type master regulator of conidiophore
FT                   development BrlA"
FT                   /id="PRO_0000445755"
FT   ZN_FING         268..292
FT                   /note="C2H2-type 1; degenerate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         300..323
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          338..368
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        344..368
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   368 AA;  41321 MW;  40262FF05651E23D CRC64;
     MQFESDFRFD MDDSFSMCSQ TLPCPSAGSS FSSASSAYEP FTPTSRRSTP NELSLDFEGA
     YNPFATHHSE LTSPSGHMSK YMFGGPVKGE PEHMSFNEAL PTTPMKKLDG MVTPDYDHML
     EMNLASRHSI GSITPSGSFP MYTISPTTMG PTSFMMTPTH SLSGSEIAES TSSWSCSNES
     PISFFPQRGL GPLELEGLDM DRHPQSPLGT YHLHAPPSPG RLRAHRKMMV HEIQRKTNEL
     QRAQIRASRK VSGSKSDGGV DVVRRAMCKC DYPGCHKAFR RNEHLKRHKQ TFHGEGPNRF
     SCEFCGKDQF NRQDNLNNHR KLHARPNSRN RGVEFIPAAV PIIEQEERSR KRRAPPKSKS
     ADKRVDDY