ID   BRLA_PEND2              Reviewed;         433 AA.
AC   K9GKQ6; J9WMK0;
DT   13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT   13-APR-2016, sequence version 2.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=C2H2 type master regulator of conidiophore development brlA {ECO:0000305};
GN   Name=brlA {ECO:0000303|PubMed:25530311}; ORFNames=PDIG_36150;
OS   Penicillium digitatum (strain PHI26 / CECT 20796) (Green mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=1170229;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RX   PubMed=23296496; DOI=10.1007/s00253-012-4593-z;
RA   Zhang T., Sun X., Xu Q., Zhu C., Li Q., Li H.;
RT   "PdSNF1, a sucrose non-fermenting protein kinase gene, is required for
RT   Penicillium digitatum conidiation and virulence.";
RL   Appl. Microbiol. Biotechnol. 97:5433-5445(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PHI26 / CECT 20796;
RX   PubMed=23171342; DOI=10.1186/1471-2164-13-646;
RA   Marcet-Houben M., Ballester A.-R., de la Fuente B., Harries E.,
RA   Marcos J.F., Gonzalez-Candelas L., Gabaldon T.;
RT   "Genome sequence of the necrotrophic fungus Penicillium digitatum, the main
RT   postharvest pathogen of citrus.";
RL   BMC Genomics 13:646-646(2012).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=25530311; DOI=10.1016/j.resmic.2014.12.003;
RA   Wang M., Sun X., Zhu C., Xu Q., Ruan R., Yu D., Li H.;
RT   "PdbrlA, PdabaA and PdwetA control distinct stages of conidiogenesis in
RT   Penicillium digitatum.";
RL   Res. Microbiol. 166:56-65(2015).
CC   -!- FUNCTION: BrlA, abaA and wetA are pivotal regulators of conidiophore
CC       development and conidium maturation (PubMed:25530311). They act
CC       individually and together to regulate their own expression and that of
CC       numerous other sporulation-specific genes (By similarity). Binds
CC       promoters of target genes at brlA response elements (BREs) containing
CC       the conserved sequence 5'-(C/A)(A/G)AGGG(G/A)-3' (By similarity).
CC       Regulates genes involved in conidiogenesis (PubMed:25530311).
CC       {ECO:0000250|UniProtKB:P22022, ECO:0000269|PubMed:25530311}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P10069}.
CC   -!- INDUCTION: Expression is positively regulated by SNF1
CC       (PubMed:23296496). {ECO:0000269|PubMed:23296496}.
CC   -!- DISRUPTION PHENOTYPE: Completely destroys asexual development,
CC       resulting in masses of vegetative hyphae that branched into the air and
CC       formation of white cotton-like colonies (PubMed:25530311).
CC       {ECO:0000269|PubMed:25530311}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EKV13796.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; JX298844; AFS18466.1; -; mRNA.
DR   EMBL; AKCT01000153; EKV13796.1; ALT_SEQ; Genomic_DNA.
DR   AlphaFoldDB; K9GKQ6; -.
DR   SMR; K9GKQ6; -.
DR   STRING; 1170229.K9GKQ6; -.
DR   EnsemblFungi; EKV13796; EKV13796; PDIG_36150.
DR   eggNOG; KOG1721; Eukaryota.
DR   HOGENOM; CLU_655506_0_0_1; -.
DR   InParanoid; K9GKQ6; -.
DR   OrthoDB; 633411at2759; -.
DR   Proteomes; UP000009882; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0048315; P:conidium formation; IEA:UniProtKB-KW.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   Pfam; PF00096; zf-C2H2; 2.
DR   SMART; SM00355; ZnF_C2H2; 2.
DR   SUPFAM; SSF57667; SSF57667; 1.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 2.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE   2: Evidence at transcript level;
KW   Activator; Conidiation; DNA-binding; Metal-binding; Nucleus;
KW   Reference proteome; Repeat; Sporulation; Transcription;
KW   Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..433
FT                   /note="C2H2 type master regulator of conidiophore
FT                   development brlA"
FT                   /id="PRO_0000435946"
FT   ZN_FING         321..345
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         351..376
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          24..49
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          240..269
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          286..306
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          391..423
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   433 AA;  49204 MW;  139BC2620C5ED883 CRC64;
     MRSHGQQISD RLTVEVDCHS LGPSDCPSMT SSFSPLDSPT PTPTSLYSQS SMASPVWHEH
     SHYHHGLPME RRTSATPLRS AFRMTDLTAG DGMMNMPCGH MDRQEQMPLP DYLPGYDDNV
     EQLWIPQEMP KTYQEPQFPY QASMPQYNQM ARNYYHRPQQ AGYLPESASN PCLSRPIFTQ
     PMERMPNSAS MTNMLHWMPS HESLVPQTIT PAQVQPYPSG PVTPPSSAYS DFPTNIPTFK
     SHTPSTPHRS VSMGTPSGSD TPVSRISGHN DYQEEFQLSP VYREGMMQRH RQPSRKPSKK
     QLLRSNLSLE NLPSIIKQVQ FKCKEPGCKG RFKRQEHLKR HMKSHSKEKP HVCWVPGCHR
     AFSRSDNLNA HYTKTHSKRG GRNRYVATLD ETSQDFDPDF RGQLTPDGRP IYGSKLEDSM
     PDCGELSVDG WDD