BRN2L_ARATH
ID BRN2L_ARATH Reviewed; 429 AA.
AC Q8GZ26; Q9LR77;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 135.
DE RecName: Full=RNA-binding protein BRN2 {ECO:0000305};
DE AltName: Full=Protein BRUNO-LIKE 2 {ECO:0000303|PubMed:23437850};
DE Short=AtBRN2 {ECO:0000303|PubMed:23437850};
GN Name=BRN2 {ECO:0000303|PubMed:23437850};
GN OrderedLocusNames=At1g03457 {ECO:0000312|Araport:AT1G03457};
GN ORFNames=F21B7.8 {ECO:0000312|EMBL:AAF86538.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23437850; DOI=10.1111/nph.12181;
RA Kim H.S., Abbasi N., Choi S.B.;
RT "Bruno-like proteins modulate flowering time via 3' UTR-dependent decay of
RT SOC1 mRNA.";
RL New Phytol. 198:747-756(2013).
CC -!- FUNCTION: RNA-binding protein involved in the regulation of flowering
CC time. Acts as repressor of the activity of SOC1, a transcriptional
CC activator of flowering time. Binds to the 3'-UTR of SOC1 mRNA in the
CC cytoplasm and participates in SOC1 mRNA decay, mediated by the distal
CC region of the SOC1 3'-UTR. {ECO:0000269|PubMed:23437850}.
CC -!- INTERACTION:
CC Q8GZ26; Q9SHZ6: UBA1A; NbExp=3; IntAct=EBI-4425532, EBI-346271;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23437850}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences. {ECO:0000305};
CC Name=1;
CC IsoId=Q8GZ26-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, flowers and siliques.
CC {ECO:0000269|PubMed:23437850}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF86538.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002560; AAF86538.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27573.1; -; Genomic_DNA.
DR EMBL; AK117246; BAC41921.1; -; mRNA.
DR EMBL; BT005358; AAO63422.1; -; mRNA.
DR PIR; B86166; B86166.
DR PIR; T00912; T00912.
DR RefSeq; NP_171845.2; NM_100228.9. [Q8GZ26-1]
DR AlphaFoldDB; Q8GZ26; -.
DR SMR; Q8GZ26; -.
DR IntAct; Q8GZ26; 3.
DR STRING; 3702.AT1G03457.2; -.
DR PaxDb; Q8GZ26; -.
DR PRIDE; Q8GZ26; -.
DR EnsemblPlants; AT1G03457.1; AT1G03457.1; AT1G03457. [Q8GZ26-1]
DR GeneID; 839497; -.
DR Gramene; AT1G03457.1; AT1G03457.1; AT1G03457. [Q8GZ26-1]
DR KEGG; ath:AT1G03457; -.
DR Araport; AT1G03457; -.
DR eggNOG; KOG0144; Eukaryota.
DR HOGENOM; CLU_015367_5_1_1; -.
DR OMA; NYPAYNA; -.
DR PhylomeDB; Q8GZ26; -.
DR PRO; PR:Q8GZ26; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GZ26; baseline and differential.
DR Genevisible; Q8GZ26; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0006402; P:mRNA catabolic process; IDA:UniProtKB.
DR GO; GO:0048573; P:photoperiodism, flowering; IMP:CACAO.
DR GO; GO:2000028; P:regulation of photoperiodism, flowering; IMP:UniProtKB.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR002343; Hud_Sxl_RNA.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 3.
DR PRINTS; PR00961; HUDSXLRNA.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 3.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Flowering; Reference proteome; Repeat;
KW Ribonucleoprotein; RNA-binding.
FT CHAIN 1..429
FT /note="RNA-binding protein BRN2"
FT /id="PRO_0000431961"
FT DOMAIN 12..93
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 100..180
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 330..408
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 410..429
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 429 AA; 47623 MW; 270E8EB4D5F3970E CRC64;
MAEETMENEE RVKLFVGQVP KHMTEIQLLT LFREFSIVNE VNIIKEKTTR APRGCCFLTC
PTREDADKVI NSFHNKKTLP GASSPLQVKY ADGELERLEH KLFVGMLPKN VSETEVQSLF
SEYGTIKDLQ ILRGSLQTSK GCLFLKYESK EQAVAAMEAL NGRHIMEGAN VPLIVKWADT
EKERQARRLL KVQSHVSRLD PQNPSMFGAL PMSYVPPYNG YGYHVPGTYG YMLPPIQTQH
AFHNVISPNQ GNGRALQGTA LTESVPPRLA PRRNFPTALG NYGYHGLQYP MAFPRGMIPP
RLPLTTVSPG ISNNGTSIPS SLQTEGPAGA NLFIYNIPRE FEDQELAATF QPFGKVLSAK
VFVDKATGIS KCFGFISYDS QAAAQNAINT MNGCQLSGKK LKVQLKRDNG QQQQQQQSKN
PLFNGLLNS
