BRN2_ARATH
ID BRN2_ARATH Reviewed; 341 AA.
AC Q9SV87;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Protein BEARSKIN2;
DE AltName: Full=NAC domain-containing protein 70;
DE Short=ANAC070;
GN Name=BRN2; Synonyms=NAC070; OrderedLocusNames=At4g10350;
GN ORFNames=F24G24.150;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=20197506; DOI=10.1105/tpc.109.072272;
RA Bennett T., van den Toorn A., Sanchez-Perez G.F., Campilho A.,
RA Willemsen V., Snel B., Scheres B.;
RT "SOMBRERO, BEARSKIN1, and BEARSKIN2 regulate root cap maturation in
RT Arabidopsis.";
RL Plant Cell 22:640-654(2010).
CC -!- FUNCTION: Transcription activator. Together with BRN1 and SMB,
CC regulates cellular maturation of root cap. Promotes the expression of
CC genes involved in secondary cell walls (SCW) biosynthesis.
CC {ECO:0000269|PubMed:20197506}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed throughout the root cap, in both
CC columella (COL) and lateral root cap (LRC) cells, with higher levels in
CC the COL-adjoining LRC than the upper LRC. Also present at low levels
CC expression in the tips of cotyledons and the cotyledon vasculature, as
CC weel as in vasculature of the first pair of true leaves and at the
CC hydathodes. {ECO:0000269|PubMed:20197506}.
CC -!- DEVELOPMENTAL STAGE: Detectable in lateral root development when they
CC reach 10 mm long. {ECO:0000269|PubMed:20197506}.
CC -!- DOMAIN: The NAC domain includes a DNA-binding domain and a dimerization
CC domain.
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DR EMBL; AL049488; CAB39788.1; -; Genomic_DNA.
DR EMBL; AL161517; CAB78158.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82870.1; -; Genomic_DNA.
DR EMBL; AY133681; AAM91615.1; -; mRNA.
DR PIR; T04050; T04050.
DR RefSeq; NP_192773.1; NM_117103.3.
DR AlphaFoldDB; Q9SV87; -.
DR SMR; Q9SV87; -.
DR STRING; 3702.AT4G10350.1; -.
DR PaxDb; Q9SV87; -.
DR PRIDE; Q9SV87; -.
DR DNASU; 826627; -.
DR EnsemblPlants; AT4G10350.1; AT4G10350.1; AT4G10350.
DR GeneID; 826627; -.
DR Gramene; AT4G10350.1; AT4G10350.1; AT4G10350.
DR KEGG; ath:AT4G10350; -.
DR Araport; AT4G10350; -.
DR TAIR; locus:2122804; AT4G10350.
DR eggNOG; ENOG502R8AM; Eukaryota.
DR HOGENOM; CLU_035664_1_3_1; -.
DR InParanoid; Q9SV87; -.
DR OMA; YQACEPG; -.
DR OrthoDB; 1138596at2759; -.
DR PhylomeDB; Q9SV87; -.
DR PRO; PR:Q9SV87; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SV87; baseline and differential.
DR Genevisible; Q9SV87; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IEP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0048829; P:root cap development; IMP:UniProtKB.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR033519; BRN1/BRN2.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR PANTHER; PTHR31989:SF368; PTHR31989:SF368; 1.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..341
FT /note="Protein BEARSKIN2"
FT /id="PRO_0000394193"
FT DOMAIN 9..160
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 109..166
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
SQ SEQUENCE 341 AA; 38946 MW; 6029D19ED4AFC187 CRC64;
MGSSSNGGVP PGFRFHPTDE ELLHYYLKKK ISYQKFEMEV IREVDLNKLE PWDLQERCKI
GSTPQNEWYF FSHKDRKYPT GSRTNRATHA GFWKATGRDK CIRNSYKKIG MRKTLVFYKG
RAPHGQKTDW IMHEYRLEDA DDPQANPSED GWVVCRVFMK KNLFKVVNEG SSSINSLDQH
NHDASNNNHA LQARSFMHRD SPYQLVRNHG AMTFELNKPD LALHQYPPIF HKPPSLGFDY
SSGLARDSES AASEGLQYQQ ACEPGLDVGT CETVASHNHQ QGLGEWAMMD RLVTCHMGNE
DSSRGITYED GNNNSSSVVQ PVPATNQLTL RSEMDFWGYS K
