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THIH_ECOLI
ID   THIH_ECOLI              Reviewed;         377 AA.
AC   P30140; Q2M8S6;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 160.
DE   RecName: Full=2-iminoacetate synthase;
DE            EC=4.1.99.19 {ECO:0000269|PubMed:17403671, ECO:0000269|PubMed:17969213};
DE   AltName: Full=Dehydroglycine synthase;
DE   AltName: Full=Tyrosine lyase;
GN   Name=thiH; OrderedLocusNames=b3990, JW3953;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=8432721; DOI=10.1128/jb.175.4.982-992.1993;
RA   Vander Horn P.B., Backstrom A.D., Stewart V., Begley T.P.;
RT   "Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in
RT   Escherichia coli K-12.";
RL   J. Bacteriol. 175:982-992(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=8265357; DOI=10.1093/nar/21.23.5408;
RA   Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.;
RT   "Analysis of the Escherichia coli genome. IV. DNA sequence of the region
RT   from 89.2 to 92.8 minutes.";
RL   Nucleic Acids Res. 21:5408-5417(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   PROTEIN SEQUENCE OF 41-47, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=10082377; DOI=10.1002/pro.5560070815;
RA   Kelleher N.L., Taylor S.V., Grannis D., Kinsland C., Chiu H.-J.,
RA   Begley T.P., McLafferty F.W.;
RT   "Efficient sequence analysis of the six gene products (7-74 kDa) from the
RT   Escherichia coli thiamin biosynthetic operon by tandem high-resolution mass
RT   spectrometry.";
RL   Protein Sci. 7:1796-1801(1998).
RN   [6]
RP   INTERACTION WITH THIG, SUBUNIT, EPR SPECTROSCOPY, AND COFACTOR.
RX   PubMed=12650933; DOI=10.1016/s0014-5793(03)00204-7;
RA   Leonardi R., Fairhurst S.A., Kriek M., Lowe D.J., Roach P.L.;
RT   "Thiamine biosynthesis in Escherichia coli: isolation and initial
RT   characterisation of the ThiGH complex.";
RL   FEBS Lett. 539:95-99(2003).
RN   [7]
RP   FUNCTION, CATALYTIC ACTIVITY, REACTION PRODUCTS, AND REACTION MECHANISM.
RX   PubMed=17969213; DOI=10.1002/anie.200702554;
RA   Kriek M., Martins F., Challand M.R., Croft A., Roach P.L.;
RT   "Thiamine biosynthesis in Escherichia coli: identification of the
RT   intermediate and by-product derived from tyrosine.";
RL   Angew. Chem. Int. Ed. 46:9223-9226(2007).
RN   [8]
RP   FUNCTION, EPR SPECTROSCOPY, COFACTOR, CATALYTIC ACTIVITY, AND REACTION
RP   MECHANISM.
RX   PubMed=17403671; DOI=10.1074/jbc.m700782200;
RA   Kriek M., Martins F., Leonardi R., Fairhurst S.A., Lowe D.J., Roach P.L.;
RT   "Thiazole synthase from Escherichia coli: an investigation of the
RT   substrates and purified proteins required for activity in vitro.";
RL   J. Biol. Chem. 282:17413-17423(2007).
CC   -!- FUNCTION: Catalyzes the radical-mediated cleavage of tyrosine to 2-
CC       iminoacetate and 4-cresol. {ECO:0000269|PubMed:17403671,
CC       ECO:0000269|PubMed:17969213}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-tyrosine + NADPH + S-adenosyl-L-methionine = 2-iminoacetate
CC         + 4-methylphenol + 5'-deoxyadenosine + L-methionine + NADP(+);
CC         Xref=Rhea:RHEA:26361, ChEBI:CHEBI:17319, ChEBI:CHEBI:17847,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:57844, ChEBI:CHEBI:58315,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:59789, ChEBI:CHEBI:77846;
CC         EC=4.1.99.19; Evidence={ECO:0000269|PubMed:17403671,
CC         ECO:0000269|PubMed:17969213};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000269|PubMed:12650933, ECO:0000269|PubMed:17403671};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated
CC       with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000269|PubMed:12650933, ECO:0000269|PubMed:17403671};
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis.
CC   -!- SUBUNIT: Forms a heterodimer with ThiG. {ECO:0000269|PubMed:12650933}.
CC   -!- INTERACTION:
CC       P30140; P30139: thiG; NbExp=2; IntAct=EBI-1125553, EBI-547059;
CC   -!- MISCELLANEOUS: The product 2-iminoacetate hydrates in vitro to yield
CC       glyoxylate and ammonium.
CC   -!- SIMILARITY: Belongs to the radical SAM superfamily. ThiH family.
CC       {ECO:0000305}.
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DR   EMBL; M88701; AAB95619.1; -; Genomic_DNA.
DR   EMBL; U00006; AAC43088.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76964.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77330.1; -; Genomic_DNA.
DR   PIR; S35121; S35121.
DR   RefSeq; NP_418417.1; NC_000913.3.
DR   RefSeq; WP_000847447.1; NZ_SSZK01000047.1.
DR   AlphaFoldDB; P30140; -.
DR   SMR; P30140; -.
DR   BioGRID; 4262909; 13.
DR   ComplexPortal; CPX-2135; thiG-thiH thiazole phosphate synthase complex.
DR   DIP; DIP-6869N; -.
DR   IntAct; P30140; 4.
DR   STRING; 511145.b3990; -.
DR   PaxDb; P30140; -.
DR   PRIDE; P30140; -.
DR   EnsemblBacteria; AAC76964; AAC76964; b3990.
DR   EnsemblBacteria; BAE77330; BAE77330; BAE77330.
DR   GeneID; 948494; -.
DR   KEGG; ecj:JW3953; -.
DR   KEGG; eco:b3990; -.
DR   PATRIC; fig|1411691.4.peg.2722; -.
DR   EchoBASE; EB1548; -.
DR   eggNOG; COG0502; Bacteria.
DR   HOGENOM; CLU_046249_1_0_6; -.
DR   InParanoid; P30140; -.
DR   OMA; LICAYRL; -.
DR   PhylomeDB; P30140; -.
DR   BioCyc; EcoCyc:THIH-MON; -.
DR   BioCyc; MetaCyc:THIH-MON; -.
DR   BRENDA; 4.1.99.19; 2026.
DR   UniPathway; UPA00060; -.
DR   PRO; PR:P30140; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:1902508; C:2-iminoacetate synthase complex; IPI:ComplexPortal.
DR   GO; GO:0036355; F:2-iminoacetate synthase activity; IDA:EcoCyc.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:EcoCyc.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR   GO; GO:0009228; P:thiamine biosynthetic process; IMP:EcoCyc.
DR   GO; GO:0009229; P:thiamine diphosphate biosynthetic process; IDA:ComplexPortal.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR010722; BATS_dom.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR012726; ThiH.
DR   InterPro; IPR034428; ThiH/NoCL/HydG-like.
DR   PANTHER; PTHR43583; PTHR43583; 1.
DR   PANTHER; PTHR43583:SF1; PTHR43583:SF1; 1.
DR   Pfam; PF06968; BATS; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDF00301; 2-iminoacetate_synthase_(ThiH); 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SMART; SM00876; BATS; 1.
DR   TIGRFAMs; TIGR02351; thiH; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   1: Evidence at protein level;
KW   4Fe-4S; Direct protein sequencing; Iron; Iron-sulfur; Lyase; Metal-binding;
KW   NADP; Reference proteome; S-adenosyl-L-methionine; Thiamine biosynthesis.
FT   CHAIN           1..377
FT                   /note="2-iminoacetate synthase"
FT                   /id="PRO_0000072514"
FT   DOMAIN          71..301
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         85
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000250"
FT   BINDING         89
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000250"
FT   BINDING         92
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   377 AA;  43320 MW;  F45A0859372F5ED6 CRC64;
     MKTFSDRWRQ LDWDDIRLRI NGKTAADVER ALNASQLTRD DMMALLSPAA SGYLEQLAQR
     AQRLTRQRFG NTVSFYVPLY LSNLCANDCT YCGFSMSNRI KRKTLDEADI ARESAAIREM
     GFEHLLLVTG EHQAKVGMDY FRRHLPALRE QFSSLQMEVQ PLAETEYAEL KQLGLDGVMV
     YQETYHEATY ARHHLKGKKQ DFFWRLETPD RLGRAGIDKI GLGALIGLSD NWRVDSYMVA
     EHLLWLQQHY WQSRYSVSFP RLRPCTGGIE PASIMDERQL VQTICAFRLL APEIELSLST
     RESPWFRDRV IPLAINNVSA FSKTQPGGYA DNHPELEQFS PHDDRRPEAV AAALTAQGLQ
     PVWKDWDSYL GRASQRL
 
 
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