BRNQL_STAA8
ID BRNQL_STAA8 Reviewed; 447 AA.
AC Q2FYM4; O34007;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Putative branched-chain amino acid carrier protein SAOUHSC_01411;
GN OrderedLocusNames=SAOUHSC_01411;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND NACL SENSITIVE PHENOTYPE.
RX PubMed=9464420; DOI=10.1128/aem.64.2.763-767.1998;
RA Vijaranakul U., Xiong A., Lockwood K., Jayaswal R.K.;
RT "Cloning and nucleotide sequencing of a Staphylococcus aureus gene encoding
RT a branched-chain-amino-acid transporter.";
RL Appl. Environ. Microbiol. 64:763-767(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
RN [3]
RP VIRULENCE STUDIES.
RX PubMed=15664928; DOI=10.1128/iai.73.2.872-877.2005;
RA Begun J., Sifri C.D., Goldman S., Calderwood S.B., Ausubel F.M.;
RT "Staphylococcus aureus virulence factors identified by using a high-
RT throughput Caenorhabditis elegans-killing model.";
RL Infect. Immun. 73:872-877(2005).
CC -!- FUNCTION: Component of the transport system for branched-chain amino
CC acids (leucine, isoleucine and valine), which is coupled to a proton
CC motive force (Potential). Contributes to NaCl tolerance. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- MISCELLANEOUS: In Caenorhabditis elegans-killing model mutant strain is
CC attenuated in virulence.
CC -!- SIMILARITY: Belongs to the branched chain amino acid transporter
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC38146.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U87144; AAC38146.1; ALT_FRAME; Genomic_DNA.
DR EMBL; CP000253; ABD30505.1; -; Genomic_DNA.
DR RefSeq; WP_001039275.1; NZ_LS483365.1.
DR RefSeq; YP_499938.1; NC_007795.1.
DR AlphaFoldDB; Q2FYM4; -.
DR STRING; 1280.SAXN108_1426; -.
DR EnsemblBacteria; ABD30505; ABD30505; SAOUHSC_01411.
DR GeneID; 3920643; -.
DR KEGG; sao:SAOUHSC_01411; -.
DR PATRIC; fig|93061.5.peg.1291; -.
DR eggNOG; COG1114; Bacteria.
DR HOGENOM; CLU_036807_0_1_9; -.
DR OMA; IWPAGPI; -.
DR PRO; PR:Q2FYM4; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015188; F:L-isoleucine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015190; F:L-leucine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005304; F:L-valine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015818; P:isoleucine transport; IBA:GO_Central.
DR GO; GO:0015820; P:leucine transport; IBA:GO_Central.
DR GO; GO:0015829; P:valine transport; IBA:GO_Central.
DR InterPro; IPR004685; Brnchd-chn_aa_trnsp_Livcs.
DR PANTHER; PTHR30588; PTHR30588; 1.
DR Pfam; PF05525; Branch_AA_trans; 1.
DR TIGRFAMs; TIGR00796; livcs; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..447
FT /note="Putative branched-chain amino acid carrier protein
FT SAOUHSC_01411"
FT /id="PRO_0000294018"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..402
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 27
FT /note="N -> I (in Ref. 1; AAC38146)"
FT /evidence="ECO:0000305"
FT CONFLICT 32..35
FT /note="SGQF -> IGSI (in Ref. 1; AAC38146)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="S -> T (in Ref. 1; AAC38146)"
FT /evidence="ECO:0000305"
FT CONFLICT 200
FT /note="I -> M (in Ref. 1; AAC38146)"
FT /evidence="ECO:0000305"
FT CONFLICT 208
FT /note="I -> D (in Ref. 1; AAC38146)"
FT /evidence="ECO:0000305"
FT CONFLICT 298
FT /note="A -> R (in Ref. 1; AAC38146)"
FT /evidence="ECO:0000305"
FT CONFLICT 316
FT /note="R -> K (in Ref. 1; AAC38146)"
FT /evidence="ECO:0000305"
FT CONFLICT 415
FT /note="Y -> C (in Ref. 1; AAC38146)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 447 AA; 48815 MW; 2B7384DF8C6B2745 CRC64;
MNKNTWVIGF TLFAMFFGAG NLIFPPNLGL DSGQFFWPAI LAFVLTGIGL PLLGVIVGAL
DKEGYIGALN KISPKFSILF LIIIYLTIGP LFAIPRTAST SFEMTITPII HSNSSIALFI
FTIIYFIVVL YICLNPSKLI DRIGSLLTPL LLITILAMII KGYLDFSGNS AGKGNEALYH
SNFSSFAEGF TQGYLTMDAI AAIAFSMIVV NAVKLTGITK TNQIFKQTLT AGLIAAVALI
FIYISLGYIG NHMPVSDMTL DQLKSKDRNI GTYLLTTMAS TGFGSFGKYL LGIIVALACL
TTACGLIVAV SEYFHRIVPK VSYKAFVLVF ILMSFIIANQ GLNAVISMSI PVLSIVYPVA
ITVVLLILIA KFIPTKRISQ QIPVIIVFIL SIFSVISKLG WLKINFIESL PLRAYSLEWF
PVAIIATILG YLVGIFVKQD PIKYQQE
