BRNQ_ECOLI
ID BRNQ_ECOLI Reviewed; 439 AA.
AC P0AD99; P37011; P77112; P77492; Q2MC25;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Branched-chain amino acid transport system 2 carrier protein;
DE AltName: Full=LIV-II;
GN Name=brnQ; OrderedLocusNames=b0401, JW0391;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-70.
RC STRAIN=K12;
RX PubMed=3550103; DOI=10.1016/0022-2836(86)90275-5;
RA Makino K., Shinagawa H., Amemura M., Nakata A.;
RT "Nucleotide sequence of the phoR gene, a regulatory gene for the phosphate
RT regulon of Escherichia coli.";
RL J. Mol. Biol. 192:549-556(1986).
RN [5]
RP IDENTIFICATION.
RX PubMed=7984428; DOI=10.1093/nar/22.22.4756;
RA Borodovsky M., Rudd K.E., Koonin E.V.;
RT "Intrinsic and extrinsic approaches for detecting genes in a bacterial
RT genome.";
RL Nucleic Acids Res. 22:4756-4767(1994).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Component of the LIV-II transport system for branched-chain
CC amino acids. This LIV-II transport system may be H(+)-coupled.
CC -!- INTERACTION:
CC P0AD99; P08312: pheS; NbExp=5; IntAct=EBI-555687, EBI-555676;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the branched chain amino acid transporter
CC family. {ECO:0000305}.
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DR EMBL; U73857; AAB18125.1; -; Genomic_DNA.
DR EMBL; U82664; AAB40157.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73504.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76181.1; -; Genomic_DNA.
DR EMBL; X04704; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A64769; A64769.
DR RefSeq; NP_414935.1; NC_000913.3.
DR RefSeq; WP_000149639.1; NZ_SSZK01000009.1.
DR AlphaFoldDB; P0AD99; -.
DR BioGRID; 4259819; 39.
DR BioGRID; 849431; 1.
DR DIP; DIP-48078N; -.
DR IntAct; P0AD99; 1.
DR STRING; 511145.b0401; -.
DR TCDB; 2.A.26.1.10; the branched chain amino acid:cation symporter (livcs) family.
DR PaxDb; P0AD99; -.
DR PRIDE; P0AD99; -.
DR EnsemblBacteria; AAC73504; AAC73504; b0401.
DR EnsemblBacteria; BAE76181; BAE76181; BAE76181.
DR GeneID; 66671300; -.
DR GeneID; 945042; -.
DR KEGG; ecj:JW0391; -.
DR KEGG; eco:b0401; -.
DR PATRIC; fig|1411691.4.peg.1876; -.
DR EchoBASE; EB2087; -.
DR eggNOG; COG1114; Bacteria.
DR HOGENOM; CLU_036807_0_0_6; -.
DR InParanoid; P0AD99; -.
DR OMA; IWPAGPI; -.
DR PhylomeDB; P0AD99; -.
DR BioCyc; EcoCyc:BRNQ-MON; -.
DR BioCyc; MetaCyc:BRNQ-MON; -.
DR PRO; PR:P0AD99; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015188; F:L-isoleucine transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0015190; F:L-leucine transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0005304; F:L-valine transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0015803; P:branched-chain amino acid transport; IMP:EcoliWiki.
DR GO; GO:0015818; P:isoleucine transport; IMP:EcoliWiki.
DR GO; GO:0015820; P:leucine transport; IMP:EcoliWiki.
DR GO; GO:0015829; P:valine transport; IMP:EcoliWiki.
DR InterPro; IPR004685; Brnchd-chn_aa_trnsp_Livcs.
DR PANTHER; PTHR30588; PTHR30588; 1.
DR Pfam; PF05525; Branch_AA_trans; 1.
DR TIGRFAMs; TIGR00796; livcs; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..439
FT /note="Branched-chain amino acid transport system 2 carrier
FT protein"
FT /id="PRO_0000099765"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..45
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..79
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 101..118
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 140..149
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 171..189
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..226
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 248..277
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 299..316
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 338
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 360..369
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 370..390
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 391..404
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 426..439
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 439 AA; 46209 MW; B30C3B5755738E0E CRC64;
MTHQLRSRDI IALGFMTFAL FVGAGNIIFP PMVGLQAGEH VWTAAFGFLI TAVGLPVLTV
VALAKVGGGV DSLSTPIGKV AGVLLATVCY LAVGPLFATP RTATVSFEVG IAPLTGDSAL
PLFIYSLVYF AIVILVSLYP GKLLDTVGNF LAPLKIIALV ILSVAAIVWP AGSISTATEA
YQNAAFSNGF VNGYLTMDTL GAMVFGIVIV NAARSRGVTE ARLLTRYTVW AGLMAGVGLT
LLYLALFRLG SDSASLVDQS ANGAAILHAY VQHTFGGGGS FLLAALIFIA CLVTAVGLTC
ACAEFFAQYV PLSYRTLVFI LGGFSMVVSN LGLSQLIQIS VPVLTAIYPP CIALVVLSFT
RSWWHNSSRV IAPPMFISLL FGILDGIKAS AFSDILPSWA QRLPLAEQGL AWLMPTVVMV
VLAIIWDRAA GRQVTSSAH
