THIKB_CANTR
ID THIKB_CANTR Reviewed; 408 AA.
AC P33291;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=3-ketoacyl-CoA thiolase B, peroxisomal;
DE EC=2.3.1.16;
DE AltName: Full=Acetyl-CoA acyltransferase B;
DE AltName: Full=Beta-ketothiolase B;
DE AltName: Full=Peroxisomal 3-oxoacyl-CoA thiolase B;
DE AltName: Full=Thiolase IB;
DE Flags: Precursor;
OS Candida tropicalis (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=5482;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 20336 / pK233 / NCYC 997;
RX PubMed=1362382; DOI=10.1111/j.1432-1033.1992.tb17505.x;
RA Kurihara T., Ueda M., Kanayama N., Kondo J., Teranishi Y., Tanaka A.;
RT "Peroxisomal acetoacetyl-CoA thiolase of an n-alkane-utilizing yeast,
RT Candida tropicalis.";
RL Eur. J. Biochem. 210:999-1005(1992).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + an acyl-CoA = a 3-oxoacyl-CoA + CoA;
CC Xref=Rhea:RHEA:21564, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:90726; EC=2.3.1.16;
CC -!- PATHWAY: Lipid metabolism; fatty acid metabolism.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Peroxisome.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC {ECO:0000305}.
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DR EMBL; D17321; BAA04143.1; -; Genomic_DNA.
DR AlphaFoldDB; P33291; -.
DR SMR; P33291; -.
DR VEuPathDB; FungiDB:CTMYA2_042690; -.
DR VEuPathDB; FungiDB:CTRG_01068; -.
DR UniPathway; UPA00199; -.
DR GO; GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
DR GO; GO:0003988; F:acetyl-CoA C-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00751; thiolase; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR002155; Thiolase.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR020615; Thiolase_acyl_enz_int_AS.
DR InterPro; IPR020610; Thiolase_AS.
DR InterPro; IPR020617; Thiolase_C.
DR InterPro; IPR020613; Thiolase_CS.
DR InterPro; IPR020616; Thiolase_N.
DR Pfam; PF02803; Thiolase_C; 1.
DR Pfam; PF00108; Thiolase_N; 1.
DR PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR TIGRFAMs; TIGR01930; AcCoA-C-Actrans; 1.
DR PROSITE; PS00098; THIOLASE_1; 1.
DR PROSITE; PS00737; THIOLASE_2; 1.
DR PROSITE; PS00099; THIOLASE_3; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Fatty acid metabolism; Lipid metabolism; Peroxisome;
KW Transferase; Transit peptide.
FT TRANSIT 1..?
FT /note="Peroxisome"
FT CHAIN ?..408
FT /note="3-ketoacyl-CoA thiolase B, peroxisomal"
FT /id="PRO_0000034076"
FT ACT_SITE 112
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000250"
FT ACT_SITE 366
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10020"
FT ACT_SITE 394
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10020"
SQ SEQUENCE 408 AA; 43270 MW; 23F3A3281C1E204B CRC64;
MDRLNQLSGQ LKPNAKQSIL QKNPDDVVIV AAYRTAIGKG FKGSFRNVHS EFILTEFLKE
FIKKTNIDPS LIEDVAIGNV LNQAAGATEH RGACLAAGIP YTAAFIAVNR FCSSGLMAIS
DIANKIKTGE IECGLAGGAE SMSTNYRDPR VAPRIDPHLA DDAQMEKCLI PMGITNENVA
NQFNISRERQ DEFAAKSYNK AAKAVASGAF KSEILPIRSI IRNSDGTEKE IIVDTDEGPR
EGVTAESLGK LRPAFDGTTT AGNASQVSDG AAAVLLMKRS LAEAKGYPII GKYVLCSTAG
VPPEIMGVGP AFAIPEVLKR TGLTVDDIDV FEINEAFAAQ CLYSAEQVNV PEEKLNINGG
AIALGHPLGE TGARQYATII PLLKPGQIGL TSMCIGSGMG SASILVRE
