THIL1_DICDI
ID THIL1_DICDI Reviewed; 414 AA.
AC Q86AD9; Q55AV9;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Probable acetyl-CoA acetyltransferase;
DE EC=2.3.1.9;
DE AltName: Full=Acetoacetyl-CoA thiolase;
GN ORFNames=DDB_G0271544;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Ohmachi T., Tanaka T.;
RT "Cloning and expression of mitochondrial acetoacetyl-CoA thiolase from
RT Dictyostelium discoideum.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 acetyl-CoA = acetoacetyl-CoA + CoA; Xref=Rhea:RHEA:21036,
CC ChEBI:CHEBI:57286, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.9;
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC {ECO:0000305}.
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DR EMBL; AB212872; BAD98242.1; -; mRNA.
DR EMBL; AAFI02000006; EAL71636.1; -; Genomic_DNA.
DR RefSeq; XP_645587.1; XM_640495.1.
DR AlphaFoldDB; Q86AD9; -.
DR SMR; Q86AD9; -.
DR STRING; 44689.DDB0231621; -.
DR PaxDb; Q86AD9; -.
DR EnsemblProtists; EAL71636; EAL71636; DDB_G0271544.
DR GeneID; 8618040; -.
DR KEGG; ddi:DDB_G0271544; -.
DR dictyBase; DDB_G0271544; acat.
DR eggNOG; KOG1390; Eukaryota.
DR HOGENOM; CLU_031026_0_1_1; -.
DR InParanoid; Q86AD9; -.
DR OMA; TNVCCTT; -.
DR PhylomeDB; Q86AD9; -.
DR BRENDA; 2.3.1.9; 1939.
DR Reactome; R-DDI-70895; Branched-chain amino acid catabolism.
DR Reactome; R-DDI-77108; Utilization of Ketone Bodies.
DR Reactome; R-DDI-77111; Synthesis of Ketone Bodies.
DR PRO; PR:Q86AD9; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0005739; C:mitochondrion; IDA:dictyBase.
DR GO; GO:0005777; C:peroxisome; IDA:dictyBase.
DR GO; GO:0003985; F:acetyl-CoA C-acetyltransferase activity; IDA:dictyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006696; P:ergosterol biosynthetic process; ISS:dictyBase.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IBA:GO_Central.
DR CDD; cd00751; thiolase; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR002155; Thiolase.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR020615; Thiolase_acyl_enz_int_AS.
DR InterPro; IPR020617; Thiolase_C.
DR InterPro; IPR020613; Thiolase_CS.
DR InterPro; IPR020616; Thiolase_N.
DR Pfam; PF02803; Thiolase_C; 1.
DR Pfam; PF00108; Thiolase_N; 1.
DR PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR TIGRFAMs; TIGR01930; AcCoA-C-Actrans; 1.
DR PROSITE; PS00098; THIOLASE_1; 1.
DR PROSITE; PS00737; THIOLASE_2; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Metal-binding; Potassium; Reference proteome; Transferase.
FT CHAIN 1..414
FT /note="Probable acetyl-CoA acetyltransferase"
FT /id="PRO_0000327943"
FT ACT_SITE 110
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000250"
FT ACT_SITE 370
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 400
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 205
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 205
FT /ligand="K(+)"
FT /ligand_id="ChEBI:CHEBI:29103"
FT /evidence="ECO:0000250"
FT BINDING 244..246
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 249
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 266
FT /ligand="K(+)"
FT /ligand_id="ChEBI:CHEBI:29103"
FT /evidence="ECO:0000250"
FT BINDING 268
FT /ligand="K(+)"
FT /ligand_id="ChEBI:CHEBI:29103"
FT /evidence="ECO:0000250"
FT BINDING 269
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 366
FT /ligand="K(+)"
FT /ligand_id="ChEBI:CHEBI:29103"
FT /evidence="ECO:0000250"
SQ SEQUENCE 414 AA; 43466 MW; B4D9267F4A4590CB CRC64;
MISNLSKVLN SNVKRMYTTA KNLESVVIVS AVRTPIGSIG GSLSTIPGTK LSSITIEEAV
KRAGIKPSDV DEAIIGNVIS ANLGQAPARQ CALGAGLEQK TITTTINKVC SSGMKAIVFG
AQSIALGHSK TVVAGGFESM SQVPYYADKM RFGAKYGNQT FIDGLVRDGL ADAYNGSAMG
VCGDDCADKY KITREQQDKF AVDSYLRALE AQKNGFFNDE IVQVPIVGRG GKVTYVVEDE
EPKKVLFDKI PNLKPAFTPN GTVTPANASK LNDGASSVIL MSESHAKELG LKPLARIIGY
ADAEQAPIEF PTAPALAIPK ALKNAGINMS QVDLFEINEA FAVVGLANAK ILDIDHNKLN
VNGGAVALGH PIGSSGCRIV VTLTHLLQNK NLKYGVAAIC NGGGGSTALV LEKL
