ID   BRO1_ASHGO              Reviewed;         834 AA.
AC   Q74ZJ6;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Vacuolar protein-sorting protein BRO1;
DE   AltName: Full=BRO domain-containing protein 1;
GN   Name=BRO1; OrderedLocusNames=AGR204W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 239 AND 755.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Involved in concentration and sorting of cargo proteins of
CC       the multivesicular body (MVB) for incorporation into intralumenal
CC       vesicles. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BRO1 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE016820; AAS54694.2; -; Genomic_DNA.
DR   RefSeq; NP_986870.2; NM_211932.2.
DR   AlphaFoldDB; Q74ZJ6; -.
DR   SMR; Q74ZJ6; -.
DR   STRING; 33169.AAS54694; -.
DR   EnsemblFungi; AAS54694; AAS54694; AGOS_AGR204W.
DR   GeneID; 4623172; -.
DR   KEGG; ago:AGOS_AGR204W; -.
DR   eggNOG; KOG2220; Eukaryota.
DR   HOGENOM; CLU_321635_0_0_1; -.
DR   InParanoid; Q74ZJ6; -.
DR   OMA; HLRNNAN; -.
DR   Proteomes; UP000000591; Chromosome VII.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR   Gene3D; 1.25.40.280; -; 1.
DR   InterPro; IPR025304; ALIX_V_dom.
DR   InterPro; IPR045251; BRO1-like.
DR   InterPro; IPR004328; BRO1_dom.
DR   InterPro; IPR038499; BRO1_sf.
DR   PANTHER; PTHR23030; PTHR23030; 1.
DR   Pfam; PF13949; ALIX_LYPXL_bnd; 1.
DR   Pfam; PF03097; BRO1; 1.
DR   SMART; SM01041; BRO1; 1.
DR   PROSITE; PS51180; BRO1; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Endosome; Protein transport; Reference proteome;
KW   Transport.
FT   CHAIN           1..834
FT                   /note="Vacuolar protein-sorting protein BRO1"
FT                   /id="PRO_0000218859"
FT   DOMAIN          4..409
FT                   /note="BRO1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00526"
FT   REGION          730..761
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          774..822
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          551..590
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   834 AA;  94362 MW;  ADBD04DCEF918BAA CRC64;
     MKTALIGLKL KDTEPLDWSK ALASYLKRNY GQHQWSQFYD DKKSAELDQL RISANSDLGL
     EALLEQNLRY YGFLEQLYLR LGSQSVQLKL DYTWYDAEHG MNGGQKYKQH TLVFEKSSTL
     FNIGVLLAQL AGEQLRASYK EAIPQLARAV ACFEYMSETF LNSPSVDLQA ENTGFLAALL
     HAEAQELFLL NVINGGDAAR RASLLCRLSY TCARFYRKCV EFYKADGADE AAAAVPYGEA
     KWRDVVNMKM HLYEGVAAYN SALVLEQAGK IGHAVGLLEK AAASMATANT HRKALQGTAY
     LRDEVDLDGI SSVVNEKLKT TAKDNDYIYH DHVPSDISLD STKLMDAIKV PEFDAVVGPY
     LEAATETCND LFRGIVPMAV YERESMYTEE KTQLLRREVD AVEQADWEYQ SFVDFTNLPK
     LLDDLERRFL DTSSSSEDAE VVHMREQLQS WADAVRQSSF NDIEQQSKNI LRTRNDIMAL
     LSSVPPENKE DVVKIKSSLL QASQSDEKLF SQVKPYVNEI QLLNNPTLLW NNFNTFTTSQ
     PTPDLLDLDH SKADEVLAKI RQIRQLYENL KLLKEERTSI MKDLKDLVNQ DDITKQLILN
     NNKSDSQIKT LFQEELEKFR PFGSRIEATV FKQGNTIKDI KIGLDTIFKL ADVQEKTSAQ
     KQAASQRKEF FSKLQKAATA FKLFETDLPK GLSFYDLLYK MTKEMHDLSV TKRTTDSDLA
     SNMAGLNISS SKETAKYNPQ PLTAPPLAPR TYMPPPQAYN RNLDIRNANT YGNNLNAGSL
     GQRPRLPQKI PTGISGLETQ NDPKLSEEES IRNPTNFYNT PSVFNESMYS KFSH