ID   THIL_PIG                Reviewed;          26 AA.
AC   P14610;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Acetyl-CoA acetyltransferase;
DE            EC=2.3.1.9;
DE   AltName: Full=Acetoacetyl-CoA thiolase;
DE   Flags: Fragment;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Heart;
RX   PubMed=5477295; DOI=10.1111/j.1432-1033.1970.tb01108.x;
RA   Gehring U., Harris J.I.;
RT   "The active site cysteines of thiolase.";
RL   Eur. J. Biochem. 16:492-498(1970).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 acetyl-CoA = acetoacetyl-CoA + CoA; Xref=Rhea:RHEA:21036,
CC         ChEBI:CHEBI:57286, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.9;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10020};
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- PTM: Succinylation, adjacent to a coenzyme A binding site.
CC       Desuccinylated by SIRT5 (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P14610; -.
DR   SMR; P14610; -.
DR   PeptideAtlas; P14610; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003985; F:acetyl-CoA C-acetyltransferase activity; IEA:UniProtKB-EC.
PE   1: Evidence at protein level;
KW   Acyltransferase; Direct protein sequencing; Mitochondrion;
KW   Reference proteome; Transferase.
FT   CHAIN           <1..>26
FT                   /note="Acetyl-CoA acetyltransferase"
FT                   /id="PRO_0000206408"
FT   ACT_SITE        21
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
FT   NON_TER         26
SQ   SEQUENCE   26 AA;  2561 MW;  7A4D306BC9BE35CE CRC64;
     QAVLGAGLPC NTTTSPIIKV CASGMK