BRO1_CANAL
ID BRO1_CANAL Reviewed; 945 AA.
AC Q5AJC1; A0A1D8PJA7;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2017, sequence version 2.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Vacuolar protein-sorting protein BRO1;
DE AltName: Full=BRO domain-containing protein 1;
GN Name=BRO1; OrderedLocusNames=CAALFM_C301750CA;
GN ORFNames=CaO19.1670, CaO19.9239;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- FUNCTION: Involved in concentration and sorting of cargo proteins of
CC the multivesicular body (MVB) for incorporation into intralumenal
CC vesicles. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the BRO1 family. {ECO:0000305}.
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DR EMBL; CP017625; AOW28216.1; -; Genomic_DNA.
DR RefSeq; XP_721747.2; XM_716654.2.
DR AlphaFoldDB; Q5AJC1; -.
DR SMR; Q5AJC1; -.
DR STRING; 237561.Q5AJC1; -.
DR PRIDE; Q5AJC1; -.
DR GeneID; 3636590; -.
DR KEGG; cal:CAALFM_C301750CA; -.
DR CGD; CAL0000196077; BRO1.
DR VEuPathDB; FungiDB:C3_01750C_A; -.
DR eggNOG; KOG2220; Eukaryota.
DR HOGENOM; CLU_003661_0_0_1; -.
DR InParanoid; Q5AJC1; -.
DR OMA; ANHKQSA; -.
DR OrthoDB; 550620at2759; -.
DR PRO; PR:Q5AJC1; -.
DR Proteomes; UP000000559; Chromosome 3.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0000815; C:ESCRT III complex; ISS:CGD.
DR GO; GO:0071285; P:cellular response to lithium ion; IMP:CGD.
DR GO; GO:0045324; P:late endosome to vacuole transport; IMP:CGD.
DR GO; GO:0006623; P:protein targeting to vacuole; ISS:CGD.
DR GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR Gene3D; 1.25.40.280; -; 1.
DR InterPro; IPR025304; ALIX_V_dom.
DR InterPro; IPR045251; BRO1-like.
DR InterPro; IPR004328; BRO1_dom.
DR InterPro; IPR038499; BRO1_sf.
DR PANTHER; PTHR23030; PTHR23030; 1.
DR Pfam; PF13949; ALIX_LYPXL_bnd; 1.
DR Pfam; PF03097; BRO1; 1.
DR SMART; SM01041; BRO1; 1.
DR PROSITE; PS51180; BRO1; 1.
PE 3: Inferred from homology;
KW Coiled coil; Cytoplasm; Endosome; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..945
FT /note="Vacuolar protein-sorting protein BRO1"
FT /id="PRO_0000218861"
FT DOMAIN 4..455
FT /note="BRO1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00526"
FT REGION 786..945
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 606..643
FT /evidence="ECO:0000255"
FT COMPBIAS 786..875
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 890..945
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 945 AA; 108853 MW; 5BB55AB4F33BC644 CRC64;
MKTHLLVVPS KKTEEVNWVK PLNNYLLSIY GNTLQYQDDI NSLNKLRQDI RGVNADDTGL
KLYYSYYSKL ELIDLRIPFH DLNKSKKLQF EWFDSFSSLP YTQNSLAFEK ANVLYNIGAI
LSRFAQFKYN ESQQLNGPEG ETAFKQSISM LQQSSGIHQF INKNFLHAPS QDLAQSTIKF
LSKLMMAQSQ EIFTLKVISG DLNQSKNSLI AKLCKSTSVL YEDCYNMINK NKKEGFKITN
ESYDGNDYED DLDDDDCDDL EGFLERPEDT EDSDNRYVAA ELDSSWISII YFKYEYYKSL
AYYFNGLHLD ANRKYGDAIA YLTKSQEVLN GVHSSTLKQV SKGSGDVYEL LDNYKYQKDA
VTIRLNDLNK DNDLIYHDIV PNLVTLPEIK PMDSTKIIKL GDNAMFHEIN EKNYNNFLVN
VVPVNIHELS SYYSEEKSQY LRNEIDHFEV SNEEVSSVLE YLKLPKALVS IKESLRGTSS
KGNTRNIDEL LSQSVIDKVN EISSQYSNDI QRKNTIENSR KEIYQLIKFL TENNCQDDAI
QLKKILYDAS VSDTKLFALV DETYHQTLGK GIQSTEFKSL FKTVDNKSSN QDSEISLLDI
IDDDDDDNTN QDKQIKNIED YLNDLNNIKQ AKFKLIEKLK KEIHNDDISD ILVLNMKLKS
TNEIKSIIFP EELKKFNPFT NELDKLITNE KSIIEQLKSR WNQLISNPQI KNLQTETNSK
NRLIISQSEK IDQFYGNWLK YHNGLIAGEK FYNQLLDQFK NFRNNIDNQL NQGMRSLNIG
QQFPTQPMLN RTQSSGLQSN YTGQSSISHS GSLSGQPSGG YNQPQPQQQQ LQQQQQQQQQ
AQFSGAYNQQ YSQPHGGYTQ FQGYGQGQGQ SQTGYQRQPP PPPPQLPPKQ SMGSYNFATT
LQQQQLPPQQ QQQPQQQRTP QNESNLIYDN PSTYNPNMYN FFSSK