BRO1_EMENI
ID BRO1_EMENI Reviewed; 1000 AA.
AC C8V212; Q5AZT6; Q6XPR4;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 03-NOV-2009, sequence version 1.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=Vacuolar protein-sorting protein bro1;
DE AltName: Full=BRO domain-containing protein 1;
GN Name=broA; Synonyms=bro1; ORFNames=AN10788;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=biA1 niiA4;
RX PubMed=1922072; DOI=10.1128/mcb.11.11.5701-5709.1991;
RA Dowzer C.E.A., Kelly J.M.;
RT "Analysis of the creA gene, a regulator of carbon catabolite repression in
RT Aspergillus nidulans.";
RL Mol. Cell. Biol. 11:5701-5709(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
CC -!- FUNCTION: Involved in concentration and sorting of cargo proteins of
CC the multivesicular body (MVB) for incorporation into intralumenal
CC vesicles. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the BRO1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAR02857.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EAA57980.1; Type=Erroneous gene model prediction; Note=The predicted gene AN6194 has been split into 2 genes: AN10778 and AN10788.; Evidence={ECO:0000305};
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DR EMBL; AY219921; AAR02857.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AACD01000105; EAA57980.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BN001301; CBF69994.1; -; Genomic_DNA.
DR AlphaFoldDB; C8V212; -.
DR SMR; C8V212; -.
DR STRING; 162425.CADANIAP00006814; -.
DR EnsemblFungi; CBF69994; CBF69994; ANIA_10788.
DR EnsemblFungi; EAA57980; EAA57980; AN6194.2.
DR VEuPathDB; FungiDB:AN10788; -.
DR eggNOG; KOG2220; Eukaryota.
DR HOGENOM; CLU_003661_1_0_1; -.
DR InParanoid; C8V212; -.
DR OMA; ANHKQSA; -.
DR OrthoDB; 550620at2759; -.
DR Proteomes; UP000000560; Chromosome I.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0071985; P:multivesicular body sorting pathway; IEA:InterPro.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.280; -; 1.
DR InterPro; IPR025304; ALIX_V_dom.
DR InterPro; IPR045251; BRO1-like.
DR InterPro; IPR004328; BRO1_dom.
DR InterPro; IPR038499; BRO1_sf.
DR PANTHER; PTHR23030; PTHR23030; 1.
DR Pfam; PF13949; ALIX_LYPXL_bnd; 1.
DR Pfam; PF03097; BRO1; 1.
DR SMART; SM01041; BRO1; 1.
DR PROSITE; PS51180; BRO1; 1.
PE 3: Inferred from homology;
KW Coiled coil; Cytoplasm; Endosome; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..1000
FT /note="Vacuolar protein-sorting protein bro1"
FT /id="PRO_0000218865"
FT DOMAIN 5..406
FT /note="BRO1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00526"
FT REGION 760..857
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 891..1000
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 732..793
FT /evidence="ECO:0000255"
FT COMPBIAS 768..789
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 810..828
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 839..853
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 893..953
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 961..993
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 262
FT /note="A -> T (in Ref. 1; AAR02857)"
FT /evidence="ECO:0000305"
FT CONFLICT 303
FT /note="D -> N (in Ref. 1; AAR02857)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1000 AA; 110592 MW; B98C24963EAA3243 CRC64;
MVQSPMISCP LKQTNEIDWI QPLKDYIRQS YGEDPERYSQ ECATLNRLRQ DMRGAGKDSA
TGRDLLYRYY GQLELLDLRF PVDENHIKIS FTWYDAFTHK PTSQYSLAFE KASIIFNISA
VLSCHAANQN RADDIGLKTA YHNFQASAGM FTYINENFLH APSTDLNRET VKTLINITLA
QGQEVFLEKQ IMDHKKAGFL AKLASQASYL YAQAIEGTQE HAKGIFDKSW VTLLQVKSAH
MGSVASYYQA LADGESGSHG VAVARLQLAE KHSTSALSWA KSLPSSISPN TNLTSEAGPS
LVDIVKFHLA NVQSQLATFV KDNDFIYHQP VPSEAGLSAV SKLPAAKAIP VSELYQGQDI
QRIIGPDIFQ KLVPMSVTET ASLYDEEKAK LIRAETEKVE TADGEMAASL DYFKLPGSLN
ILKGGMDQEV MVDEEFQRWC QELAGHDSFA KAFDTLQDRK SEVLATLDQC AKQLDLEESV
CEKMRSKYGA DWSQQPSSRL NMTLRNDIRT YRDTVHEASA SDAQLSATLR QYESDFDEMR
SAGETDEADV LFQRAMIKAG SKQGKTKNGV TSPYSATEGS LLDDVYDDGV PSVAEQIARV
ESILKKLNLV KRERTQVLKD LKEKVRNDDI SNVLILNKKS ITGQESQLFE AELEKFHPHQ
MRIVQANHKQ TALMKELTKT YGDLLQDKRV RAEQSKYESI TRQRNSVMAR YKKIYDSFNN
LGSGIKQAQT FYAEMTETVD SLKKNVDTFI NNRRSEGAQL LGQIEREKAA GTSDHEERER
EKLRQLMERL STEPKPPSVP PGSSTAGPAK AKSPPPPVKA PAYPTNIAPP KASPHFAPTV
PQQHGTPVSH SPAPYSQYVP PGAGVSYLPS QSFQQGAAAP LSEGYNPMAY PIPASSMSPP
PSQPFYSPTP TPFYTSPTPP VPSGQYMPQG YVPPPPPPRP QQPTYPPSTG PFPSGPGGYA
QSRPYGSSQH HKAHSQSSPQ TGPSVSANSS DPWAGLNAWK
