ID   BRO1_KLULA              Reviewed;         813 AA.
AC   Q6CM12;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Vacuolar protein-sorting protein BRO1;
DE   AltName: Full=BRO domain-containing protein 1;
GN   Name=BRO1; OrderedLocusNames=KLLA0E23914g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Involved in concentration and sorting of cargo proteins of
CC       the multivesicular body (MVB) for incorporation into intralumenal
CC       vesicles. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BRO1 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR382125; CAH00114.1; -; Genomic_DNA.
DR   RefSeq; XP_455027.1; XM_455027.1.
DR   AlphaFoldDB; Q6CM12; -.
DR   SMR; Q6CM12; -.
DR   STRING; 28985.XP_455027.1; -.
DR   EnsemblFungi; CAH00114; CAH00114; KLLA0_E23871g.
DR   GeneID; 2894350; -.
DR   KEGG; kla:KLLA0_E23871g; -.
DR   eggNOG; KOG2220; Eukaryota.
DR   HOGENOM; CLU_321635_0_0_1; -.
DR   InParanoid; Q6CM12; -.
DR   OMA; HLRNNAN; -.
DR   Proteomes; UP000000598; Chromosome E.
DR   GO; GO:0010008; C:endosome membrane; IEA:GOC.
DR   GO; GO:0035800; F:deubiquitinase activator activity; IEA:EnsemblFungi.
DR   GO; GO:1904669; P:ATP export; IEA:EnsemblFungi.
DR   GO; GO:0070676; P:intralumenal vesicle formation; IEA:EnsemblFungi.
DR   GO; GO:0072671; P:mitochondria-associated ubiquitin-dependent protein catabolic process; IEA:EnsemblFungi.
DR   GO; GO:0016579; P:protein deubiquitination; IEA:EnsemblFungi.
DR   GO; GO:0036010; P:protein localization to endosome; IEA:EnsemblFungi.
DR   GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IEA:EnsemblFungi.
DR   GO; GO:0007584; P:response to nutrient; IEA:EnsemblFungi.
DR   Gene3D; 1.25.40.280; -; 1.
DR   InterPro; IPR025304; ALIX_V_dom.
DR   InterPro; IPR045251; BRO1-like.
DR   InterPro; IPR004328; BRO1_dom.
DR   InterPro; IPR038499; BRO1_sf.
DR   PANTHER; PTHR23030; PTHR23030; 1.
DR   Pfam; PF13949; ALIX_LYPXL_bnd; 1.
DR   Pfam; PF03097; BRO1; 1.
DR   SMART; SM01041; BRO1; 1.
DR   PROSITE; PS51180; BRO1; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Endosome; Protein transport; Reference proteome;
KW   Transport.
FT   CHAIN           1..813
FT                   /note="Vacuolar protein-sorting protein BRO1"
FT                   /id="PRO_0000218867"
FT   DOMAIN          4..403
FT                   /note="BRO1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00526"
FT   REGION          704..729
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          752..781
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   813 AA;  92569 MW;  71F308BD0A26DB6D CRC64;
     MKTVLLPLKI KDTESISWTK GLITYLKRSY GSSQWSVFYD GDKTAEIDSC RTTANSDLAP
     ESLLDQNYKY AAILEQIYLR LGAHSGSLQM DFTWYEAEYH SRLSEKKFKQ HTVVFEKSSV
     MYNIGVLLSQ IAKKKMSDNY KSAIPYLSKA MACFDYMSNN FLNSPSIDCA AENTSFLSTL
     LHAEAQEMFL MTLINGPDAS KRASLISKLA HTALNLYEKC QEFYEDSSTA TFGTTPYGED
     KWKDIITLKV HLYRAISTYN HLRVLEEAKK IGEAIAYGKL AVHEIKEAGF YKVYVKDEID
     LTGLKAVIEE KLKSLIKDND FIYNDLIPAD VSIESIKTMD AVKPVTWEEQ LKVYLEQVVD
     LCNGAFKGIV PMEVYENESI YSEEKASLLR KEVEETDTAD WEYQSFIEFT DLPKLIKDLE
     SRYVHGGNGS SDDPQYEIMK QKITTWSKVV QDSNFKDVRA QMQNILDKRN EILTILSSVS
     PDQRENALKI KNSLVQASQS DDKIFASINP CLEEVNLLAN HQLLWSIMSK FQIRNDEPSL
     LDLDDSKNQQ ILDRLHNIKH NQEILRLLKE ERTRNLKDFK ESLNNDDITQ KLILLTGKPK
     KEMRVIFEEE LTKFKPLSTR IEATIFKQNN LINDIKIKLD EVFKLIGVQD KTPEQENTVK
     ERQTFFDKVE KAFNQFVIFS TDLPKGLNFY DTLLKMTREL SHASTPGQSV NLIDSNTLPP
     NLPQKSTQST TLTKSFANLN LCNDVNDSVT QSPANLANTN NTGVNPGPPQ LPPKPLSTGI
     SGLVSDQKLQ NGPTSFYNNP SVFDENLYSK FSK