ID   BRO1_NEUCR              Reviewed;        1012 AA.
AC   Q7SAN9;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Vacuolar protein-sorting protein bro-1;
DE   AltName: Full=BRO domain-containing protein 1;
GN   Name=bro-1; ORFNames=B2N18.270, NCU08001;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Involved in concentration and sorting of cargo proteins of
CC       the multivesicular body (MVB) for incorporation into intralumenal
CC       vesicles. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BRO1 family. {ECO:0000305}.
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DR   EMBL; BX897674; CAE85528.1; -; Genomic_DNA.
DR   EMBL; CM002239; EAA33435.1; -; Genomic_DNA.
DR   RefSeq; XP_962671.1; XM_957578.2.
DR   AlphaFoldDB; Q7SAN9; -.
DR   SMR; Q7SAN9; -.
DR   STRING; 5141.EFNCRP00000008293; -.
DR   PRIDE; Q7SAN9; -.
DR   EnsemblFungi; EAA33435; EAA33435; NCU08001.
DR   GeneID; 3878835; -.
DR   KEGG; ncr:NCU08001; -.
DR   VEuPathDB; FungiDB:NCU08001; -.
DR   HOGENOM; CLU_003661_0_0_1; -.
DR   InParanoid; Q7SAN9; -.
DR   OMA; ANHKQSA; -.
DR   Proteomes; UP000001805; Chromosome 4, Linkage Group IV.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR   Gene3D; 1.25.40.280; -; 1.
DR   InterPro; IPR025304; ALIX_V_dom.
DR   InterPro; IPR045251; BRO1-like.
DR   InterPro; IPR004328; BRO1_dom.
DR   InterPro; IPR038499; BRO1_sf.
DR   PANTHER; PTHR23030; PTHR23030; 1.
DR   Pfam; PF13949; ALIX_LYPXL_bnd; 1.
DR   Pfam; PF03097; BRO1; 1.
DR   SMART; SM01041; BRO1; 1.
DR   PROSITE; PS51180; BRO1; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Endosome; Protein transport; Reference proteome;
KW   Transport.
FT   CHAIN           1..1012
FT                   /note="Vacuolar protein-sorting protein bro-1"
FT                   /id="PRO_0000218868"
FT   DOMAIN          5..407
FT                   /note="BRO1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00526"
FT   REGION          558..578
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          784..812
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          827..1012
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          301..330
FT                   /evidence="ECO:0000255"
FT   COILED          719..775
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        785..803
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        827..862
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        889..925
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        928..946
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        968..998
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1012 AA;  112195 MW;  4D5EEAE82B302208 CRC64;
     MVQAPMISVP LKATSEIDWV APLKNYIRNT YGDDPERYAE ECATLNRLRQ DMRGAGKDST
     SGRDLLYRYY GQLELLDLRF PVDEKNIKIS FTWFDAFTHK PTAQYSLAFE KASIIFNISA
     VLSCHAAHQL RTEEAGLKTA YHSFQASAGM FTYINENFLH APSSDLSRET VKTLISIMLA
     QAQEVFLEKQ IADQKKNGLL AKLSSQAAAL YAQAVEGVQE NVTKAIFEKV WLSVVQIKLN
     FMNSLAQYYQ ALADEDANSY GVAIARLEIA QGLAKEANKM AHSFPTSVPP NSNLTSDCGH
     ILADATKRHL ATVKEKLEEL NKENDMIYHQ PVPAEASVAP VPKLPAAKPI PVSELYAGQD
     IQRITGPDLF AKIVPLAVTE SASLYDEEKA KLVRAETERV ETANSEMAAS LDYLRLPGAL
     QVLKGGFDQD ILPDEDFRTW CVDVADHESP HRIFEYLHTE KQAISTILDK SSRQLDMEES
     VCEKMRSKYD AEWTQQPSSR LTTTLRTDIR RYREALEVAA KSDGQLATKL RANETELDEM
     RQAAQHGEID ELFQRAVRKS RKSNPNSPAT VEPNLLEADF DDGGPSVVEQ IQKVEDILKK
     LSLVKKERLQ VLQDLKQKAH SDDISQILIL NKKSIANYEQ QLFQQELEKF RPHQNRLVQA
     SHKQAALMRE LTVTFNNLLQ DKRVRADQSR YESVQRSRTS VINKYKRAYQ EFLDLEAGLQ
     SAKNWYKDMR QEAESLEKNV EAFVNNRRAE GAQLLNQIEQ DRAANKSSHA ALEQERLKNL
     MERMSMDPSP TSPKPSSGSG GRPTPAPLSF APAAVSNTPL SAYQKSNFST QYPASPPATQ
     VPHNPGGQQQ TPYQQYNPSS LGRIPGPASP PPNQTSFNIG PGRHPASPPP TQTSFAQSRP
     YSLTTYGNPS ALNPQGGQPQ QSQPGGYVPP GFVPPPPPPG PPPLGPQQTV HYGGNEYYAG
     AMGNPNIGRP GSGQQGPQGQ QGGWGQPPPQ QQLYQQQGGG GGDPWAGLSA WK