ID   BRO1_USTMA              Reviewed;        1076 AA.
AC   Q4PHA8; A0A0D1D137;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Vacuolar protein-sorting protein BRO1;
DE   AltName: Full=BRO domain-containing protein 1;
GN   Name=BRO1; ORFNames=UMAG_00505;
OS   Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX   NCBI_TaxID=237631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=17080091; DOI=10.1038/nature05248;
RA   Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA   Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA   Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA   Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA   Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA   Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA   Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA   Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA   Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA   Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA   Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA   Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA   Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA   Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA   Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA   Birren B.W.;
RT   "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT   maydis.";
RL   Nature 444:97-101(2006).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=521 / FGSC 9021;
RA   Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in concentration and sorting of cargo proteins of
CC       the multivesicular body (MVB) for incorporation into intralumenal
CC       vesicles. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Endosome {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BRO1 family. {ECO:0000305}.
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DR   EMBL; CM003140; KIS72083.1; -; Genomic_DNA.
DR   RefSeq; XP_011386352.1; XM_011388050.1.
DR   AlphaFoldDB; Q4PHA8; -.
DR   SMR; Q4PHA8; -.
DR   STRING; 5270.UM00505P0; -.
DR   EnsemblFungi; KIS72083; KIS72083; UMAG_00505.
DR   GeneID; 23561785; -.
DR   KEGG; uma:UMAG_00505; -.
DR   VEuPathDB; FungiDB:UMAG_00505; -.
DR   eggNOG; KOG2220; Eukaryota.
DR   HOGENOM; CLU_003661_0_0_1; -.
DR   InParanoid; Q4PHA8; -.
DR   OMA; ANHKQSA; -.
DR   OrthoDB; 550620at2759; -.
DR   Proteomes; UP000000561; Chromosome 1.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR   Gene3D; 1.25.40.280; -; 1.
DR   InterPro; IPR025304; ALIX_V_dom.
DR   InterPro; IPR045251; BRO1-like.
DR   InterPro; IPR004328; BRO1_dom.
DR   InterPro; IPR038499; BRO1_sf.
DR   PANTHER; PTHR23030; PTHR23030; 1.
DR   Pfam; PF13949; ALIX_LYPXL_bnd; 1.
DR   Pfam; PF03097; BRO1; 1.
DR   SMART; SM01041; BRO1; 1.
DR   PROSITE; PS51180; BRO1; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Endosome; Protein transport; Reference proteome;
KW   Transport.
FT   CHAIN           1..1076
FT                   /note="Vacuolar protein-sorting protein BRO1"
FT                   /id="PRO_0000218870"
FT   DOMAIN          8..413
FT                   /note="BRO1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00526"
FT   REGION          775..1076
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          489..550
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        775..793
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        854..870
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        890..914
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        942..973
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1016..1066
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1076 AA;  115259 MW;  6AB425B1A4B4E1CA CRC64;
     MADAHQSPLL LLPLKTTEEV DLGSAVKSLI TNSYGEDSKK YSEQTSQLNR ARQDAVKGAA
     SDATGRDLLF KWFHMLEMLE LRFPELRVPF PWKDAFTQKT ISQSSLAYEK ASIIFNIAAT
     LSSLASSQPR MPGNADGLKR AYAALRQAAG MLSYINENFL HAPSTDMSKD VVKCLVGITL
     AQASEVFLEK TIEEKKGAGL ISKLASQTAA AYTGLVDDSR ENVTKGIFER SWAYLIQVKA
     RHFTSVMQYY KALADDAAGS HGACLVRLTV AETAAKEARN LLTTFSASAT ASVTADRPSL
     PSDAASALVA IVNAQVARCT ERKESAVKDN DLIYHDILPS ESSLPAVDKL VAANPIPIQE
     IFAAPEVQRV IGPDLFQNLV PLGVHEKASL YSEEKAKIAR AESERHDLAT GEMQASLDYL
     GLPSSLQKYR ALAQGSGSNA MLDSLADPGP EVMRWSTEEA EGGGGRGADG LGVGATGVDS
     ALQRIESIKS QAANDIDAAL AALDDENREC EKQRVRFGHK WSQDPAGLHT KDMRVNLKEN
     KEAMQQASQN DAQIGGLWKS IRQDVQLLVS GREALEAAFA AALTGQTGLY GEGSAPTSLI
     DTSAEEESAS EGEIASVRAK LAQIDEALIK LNKIKKERGE VLADLKEKIQ TDDISQVLVL
     NRRAQNVDSS IFAAELEKFK PHQNRIAVSL HHQQALLAEV ETAFKELIEL PASKAAGAKW
     DEKEQARNKL VARLKRARDS NAQVRAGVAK GLQFYADLQE IVKATRQNVN RYVGERKSER
     SKLVSELEWE EKGSDGLSSS MAGLSGLGGA GGHPAPPTPH RHTSYASAYG GAGAPGPLSP
     GPPPAALPGV RQTSNSGPPP PPPLPHQQAS YQQQPPPAAA APAHDPYSSM FSSGPFSAAL
     QQPASPQHQR PPTHYGSQLG YACDAPQRAP ALPPPPQQFQ PSFAPSVASP TQSRYTSPPP
     VPQGQQTFSG SSGYGMPPAP LQHGGGYGGS QPYAAHDYSS PPPQSPYGVP IGQQQYGGGH
     AQNQPQYQAY GAPPQQHASQ QVYGSRAQPP PPQQQQYQQP SYAQHGGYQG GYQPPY