ID   BROMI_DANRE             Reviewed;        1298 AA.
AC   Q5RHR6;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Protein broad-minded;
DE   AltName: Full=TBC1 domain family member 32;
GN   Name=tbc1d32; Synonyms=bromi; ORFNames=si:dkey-233p4.1;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=20159594; DOI=10.1016/j.devcel.2009.12.014;
RA   Ko H.W., Norman R.X., Tran J., Fuller K.P., Fukuda M., Eggenschwiler J.T.;
RT   "Broad-minded links cell cycle-related kinase to cilia assembly and
RT   hedgehog signal transduction.";
RL   Dev. Cell 18:237-247(2010).
CC   -!- FUNCTION: Required for high-level Shh responses in the developing
CC       neural tube. Together with cdk20, controls the structure of the primary
CC       cilium by coordinating assembly of the ciliary membrane and axoneme,
CC       allowing gli2 to be properly activated in response to SHH signaling.
CC       {ECO:0000269|PubMed:20159594}.
CC   -!- SUBUNIT: Interacts with cdk20, which promotes cdk20 stability and
CC       function. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cell projection, cilium
CC       {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Fish are viable during embryogenesis and early
CC       larval stages, but exhibit curvature of the body axis and
CC       hydrocephalus, and curled cilia in distal kidney tubules.
CC       {ECO:0000269|PubMed:20159594}.
CC   -!- CAUTION: The Rab-GAP TBC domain appears to be inactive, probably due to
CC       a lack of the essential Arg and Gln in the catalytic finger motifs.
CC       {ECO:0000305}.
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DR   EMBL; BX470129; CAI11664.2; -; Genomic_DNA.
DR   EMBL; BX470071; CAI11664.2; JOINED; Genomic_DNA.
DR   EMBL; CT955964; CAI11664.2; JOINED; Genomic_DNA.
DR   EMBL; BX470071; CAI20996.2; -; Genomic_DNA.
DR   EMBL; BX470129; CAI20996.2; JOINED; Genomic_DNA.
DR   EMBL; CT955964; CAI20996.2; JOINED; Genomic_DNA.
DR   EMBL; CT955964; CAN88284.1; -; Genomic_DNA.
DR   EMBL; BX470071; CAN88284.1; JOINED; Genomic_DNA.
DR   EMBL; BX470129; CAN88284.1; JOINED; Genomic_DNA.
DR   RefSeq; NP_001093493.1; NM_001100023.1.
DR   AlphaFoldDB; Q5RHR6; -.
DR   SMR; Q5RHR6; -.
DR   STRING; 7955.ENSDARP00000122813; -.
DR   PaxDb; Q5RHR6; -.
DR   Ensembl; ENSDART00000061168; ENSDARP00000061167; ENSDARG00000041734.
DR   Ensembl; ENSDART00000144401; ENSDARP00000122813; ENSDARG00000041734.
DR   GeneID; 566474; -.
DR   KEGG; dre:566474; -.
DR   CTD; 221322; -.
DR   ZFIN; ZDB-GENE-061106-5; tbc1d32.
DR   eggNOG; ENOG502QR93; Eukaryota.
DR   GeneTree; ENSGT00940000153528; -.
DR   HOGENOM; CLU_260246_0_0_1; -.
DR   InParanoid; Q5RHR6; -.
DR   OMA; RLHISKY; -.
DR   PhylomeDB; Q5RHR6; -.
DR   TreeFam; TF329092; -.
DR   PRO; PR:Q5RHR6; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 20.
DR   Bgee; ENSDARG00000041734; Expressed in testis and 16 other tissues.
DR   ExpressionAtlas; Q5RHR6; baseline.
DR   GO; GO:0005929; C:cilium; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0035082; P:axoneme assembly; IMP:MGI.
DR   GO; GO:0060271; P:cilium assembly; IMP:ZFIN.
DR   GO; GO:1905515; P:non-motile cilium assembly; IBA:GO_Central.
DR   InterPro; IPR032735; BROMI.
DR   InterPro; IPR039156; PHAF1/BROMI.
DR   InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR   PANTHER; PTHR13465; PTHR13465; 1.
DR   PANTHER; PTHR13465:SF3; PTHR13465:SF3; 1.
DR   Pfam; PF14961; BROMI; 1.
DR   SUPFAM; SSF47923; SSF47923; 1.
PE   3: Inferred from homology;
KW   Cell projection; Cilium; Cytoplasm; Developmental protein;
KW   Reference proteome.
FT   CHAIN           1..1298
FT                   /note="Protein broad-minded"
FT                   /id="PRO_0000360032"
FT   DOMAIN          1171..1283
FT                   /note="Rab-GAP TBC"
SQ   SEQUENCE   1298 AA;  147547 MW;  47F87FA4895B33C8 CRC64;
     MSQFSTEDEA ELQTLLRKLL KSVKDRISGA PSVECAEEIL LHLEETDKNF HNYEFVKYLR
     EYVESSLGAV IEVETENFTK GEGHAVGSGQ DTLVHAVTKN TRESTQYKQM MQTLKQTMMM
     VVESLINKFE EDQMKREEMD RKIQHQQSIS QYADNCSDSD SSFNQSYAFI KHEQLQVIAE
     KLDPGRPREV RWEALQSLCH APPSDVLSCE SWTGLRRNLS TALTDPDPEL SDKVLQFFAK
     TFSSSPLNVT KDIYASIGNT KTLEAHFLYH KLSFPSGTTS IDANRPDMAR LLKQMRLMND
     FQKEVTTFWI RHPEKYMEEI IENTLSLLAL HSEQGMSSPG SEKSLEPIHL ISLLDIKATW
     FKKWMHGYYS RTVVLRLLER KYKSLIVNAL QQCIYYFDSC DALSEETLEM IHSLEHQQIG
     PAGRTLYTSK ELEFVYFVHS LCVLGRLVMY TNGRKFFPIK VKKRRDPVTL TDLVVILINI
     MYQHPKPSCG ETALADTMSP TNLVMEVLRT LCDRTECAVE CLYQIPVIET LLAPLITLLT
     GKLPKLNSME SALTHTADTL ARIATTERGL SLLLYDRNLV SAEGESISAA HLIVQFTQKL
     LAKELQVENS SSMSGAFIFV CRQMYNTCEG LQVLRPYSLH ECIAQAWRTT SSMSERVPTP
     VPGAPPALAS QELQSVVAWE EMLLDNLLNF AATPKGLLLL QQTGAINECV TYMFSRFTKK
     LQVSRCEKFG YGVMVTQVAT TAPGIVALQS SGFVQTIVVE LWSALECGRE DVRVVHPKST
     PMDPIDRSCL KSFLSLVNLL SSPHAVWELL GHRALPNKTE YNLREMATSV LDLMDRLIII
     NSDAKIHSLF NYEQSHTFGL RLLSVLCCNL DSFLLLESQY KLTDLLLQGQ RDNVTEPPSG
     EGEFIIDGLS VERNHILVRI CAVGGPSERK LPPRALQKGN DPYPWPMVST YPLPKYYIFD
     VPKILRTKQE SEIGAFLTSS KETERDASWM DDCRRQFCKI MATKSNTLTG YVLADLLEMV
     VLHLSSSSND CFFPPAEYKV VDNSVKTRSL SSVEQLGVEI SLRYGKFLKL LREDSEQDLC
     LLLKHCQEFL SQQRVKVTSE LCGYPGHDWF ASTVFLLMHG DVGRSLSLLL RFSRLLPSAF
     LWPPRLHSSV HLPIEIAQSA IHPIYSCTAH YVEMLLKAEV PLVFSAFRMS GFTPSQICVQ
     WLGQCFWNYL DWPEICQYVT TCVIMGPDYQ VYMCVSALRH LQQDILQHTQ TQDLQVFLKE
     EPIQGFRVSN YLEYMEGLER SYRSMVLSDM RSILPRSS