BROX_MOUSE
ID BROX_MOUSE Reviewed; 411 AA.
AC Q8K2Q7; Q8BWM8; Q9DCT7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=BRO1 domain-containing protein BROX;
DE AltName: Full=BRO1 domain- and CAAX motif-containing protein;
DE Flags: Precursor;
GN Name=Brox;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Egg, Kidney, and Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: Interacts with CHMP4B. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8K2Q7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K2Q7-2; Sequence=VSP_028041, VSP_028042;
CC -!- SIMILARITY: Belongs to the BROX family. {ECO:0000305}.
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DR EMBL; AK002489; BAB22138.1; -; mRNA.
DR EMBL; AK050525; BAC34307.1; -; mRNA.
DR EMBL; AK162247; BAE36814.1; -; mRNA.
DR EMBL; BC030340; AAH30340.1; -; mRNA.
DR EMBL; BC120692; AAI20693.1; -; mRNA.
DR EMBL; BC125527; AAI25528.1; -; mRNA.
DR CCDS; CCDS56662.1; -. [Q8K2Q7-1]
DR RefSeq; NP_082137.1; NM_027861.2. [Q8K2Q7-1]
DR RefSeq; XP_006535660.1; XM_006535597.3.
DR RefSeq; XP_006535661.1; XM_006535598.3. [Q8K2Q7-1]
DR AlphaFoldDB; Q8K2Q7; -.
DR SMR; Q8K2Q7; -.
DR BioGRID; 214849; 2.
DR STRING; 10090.ENSMUSP00000061345; -.
DR iPTMnet; Q8K2Q7; -.
DR PhosphoSitePlus; Q8K2Q7; -.
DR EPD; Q8K2Q7; -.
DR MaxQB; Q8K2Q7; -.
DR PaxDb; Q8K2Q7; -.
DR PeptideAtlas; Q8K2Q7; -.
DR PRIDE; Q8K2Q7; -.
DR ProteomicsDB; 273767; -. [Q8K2Q7-1]
DR ProteomicsDB; 273768; -. [Q8K2Q7-2]
DR Antibodypedia; 34631; 31 antibodies from 14 providers.
DR DNASU; 71678; -.
DR Ensembl; ENSMUST00000057062; ENSMUSP00000061345; ENSMUSG00000046836. [Q8K2Q7-1]
DR Ensembl; ENSMUST00000163528; ENSMUSP00000132333; ENSMUSG00000046836. [Q8K2Q7-1]
DR GeneID; 71678; -.
DR KEGG; mmu:71678; -.
DR UCSC; uc008icq.1; mouse. [Q8K2Q7-1]
DR CTD; 148362; -.
DR MGI; MGI:1918928; Brox.
DR VEuPathDB; HostDB:ENSMUSG00000046836; -.
DR eggNOG; ENOG502QQBR; Eukaryota.
DR GeneTree; ENSGT00390000006681; -.
DR HOGENOM; CLU_056561_0_0_1; -.
DR InParanoid; Q8K2Q7; -.
DR OMA; HTCLRRA; -.
DR OrthoDB; 614228at2759; -.
DR PhylomeDB; Q8K2Q7; -.
DR TreeFam; TF314743; -.
DR BioGRID-ORCS; 71678; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Brox; mouse.
DR PRO; PR:Q8K2Q7; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q8K2Q7; protein.
DR Bgee; ENSMUSG00000046836; Expressed in cortical plate and 75 other tissues.
DR ExpressionAtlas; Q8K2Q7; baseline and differential.
DR Genevisible; Q8K2Q7; MM.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 1.25.40.280; -; 1.
DR InterPro; IPR004328; BRO1_dom.
DR InterPro; IPR038499; BRO1_sf.
DR InterPro; IPR038898; BROX.
DR PANTHER; PTHR23032; PTHR23032; 1.
DR Pfam; PF03097; BRO1; 1.
DR SMART; SM01041; BRO1; 1.
DR PROSITE; PS51180; BRO1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Lipoprotein; Membrane; Methylation; Prenylation;
KW Reference proteome.
FT CHAIN 1..408
FT /note="BRO1 domain-containing protein BROX"
FT /id="PRO_0000304613"
FT PROPEP 409..411
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000396737"
FT DOMAIN 90..408
FT /note="BRO1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00526"
FT REGION 375..411
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 375..403
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 408
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 408
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000250"
FT VAR_SEQ 102..103
FT /note="SA -> RF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028041"
FT VAR_SEQ 104..411
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028042"
FT CONFLICT 163
FT /note="P -> S (in Ref. 1; BAC34307)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 411 AA; 46202 MW; 5079195FBA23DF87 CRC64;
MTHWFHRNPL KATAPVSFNY YGMITGPPAS KICNDLRSAR TRLLELFTDL SCNPETMKNA
ADLYFSLLQG FINSVGDSTQ ESKLRYIQNF KWTDTLQGHV PSAQQDAVFE LISMGFNVAL
WYTKYASRLA GKENITEDEA KEVHRSLKIA AGIFKHLKES HIPKLLTPAE KGRDLEARLI
DAYIIQCQAE AQEVTIARAI ELKHAPGLIA ALAYDTASFY QKADHTLSSL EPAHSAKWRK
YLHLKMCFYT AYAYCYHGQT LLASDKCGEA IRSLQEAEKL YAEAEALCKE YGETKGPGPT
AKPSGHLFFR KLGSLVKNTL DKCQRENGFI YFQKIPTEAP QLELKANYGL VEPVPFEFPP
MSAHWTPEAL AAFDLTKRPK DDSVKPKPEE DVKPVKEPDI RPQKDTGCSV S
