BRO_DROME
ID BRO_DROME Reviewed; 213 AA.
AC Q24039; Q9W0B5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Protein brother;
GN Name=Bro; ORFNames=CG7960;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8622696; DOI=10.1128/mcb.16.3.932;
RA Golling G., Li L., Pepling M., Stebbins M., Gergen J.P.;
RT "Drosophila homologs of the proto-oncogene product PEBP2/CBF beta regulate
RT the DNA-binding properties of Runt.";
RL Mol. Cell. Biol. 16:932-942(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Regulates the DNA-binding properties of Runt.
CC -!- INTERACTION:
CC Q24039; P22814: run; NbExp=6; IntAct=EBI-148540, EBI-868309;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CBF-beta family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB03675.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U22176; AAB03675.1; ALT_INIT; mRNA.
DR EMBL; AE014296; AAF47538.3; -; Genomic_DNA.
DR RefSeq; NP_477066.2; NM_057718.3.
DR AlphaFoldDB; Q24039; -.
DR SMR; Q24039; -.
DR BioGRID; 63736; 8.
DR IntAct; Q24039; 11.
DR STRING; 7227.FBpp0072643; -.
DR PaxDb; Q24039; -.
DR DNASU; 38202; -.
DR EnsemblMetazoa; FBtr0072760; FBpp0072643; FBgn0013755.
DR GeneID; 38202; -.
DR KEGG; dme:Dmel_CG7960; -.
DR CTD; 38202; -.
DR FlyBase; FBgn0013755; Bro.
DR VEuPathDB; VectorBase:FBgn0013755; -.
DR eggNOG; KOG4785; Eukaryota.
DR HOGENOM; CLU_074992_0_1_1; -.
DR InParanoid; Q24039; -.
DR OMA; PYAQEQE; -.
DR OrthoDB; 1254153at2759; -.
DR PhylomeDB; Q24039; -.
DR SignaLink; Q24039; -.
DR BioGRID-ORCS; 38202; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38202; -.
DR PRO; PR:Q24039; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0013755; Expressed in cleaving embryo and 2 other tissues.
DR ExpressionAtlas; Q24039; baseline and differential.
DR Genevisible; Q24039; DM.
DR GO; GO:0016513; C:core-binding factor complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:FlyBase.
DR GO; GO:0008134; F:transcription factor binding; TAS:FlyBase.
DR GO; GO:0048749; P:compound eye development; TAS:FlyBase.
DR GO; GO:0035206; P:regulation of hemocyte proliferation; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 2.40.250.10; -; 1.
DR InterPro; IPR003417; CBF_beta.
DR InterPro; IPR036552; CBF_bsu_sf.
DR PANTHER; PTHR10276; PTHR10276; 1.
DR Pfam; PF02312; CBF_beta; 1.
DR SUPFAM; SSF50723; SSF50723; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..213
FT /note="Protein brother"
FT /id="PRO_0000064990"
FT REGION 189..213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 190..204
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 10
FT /note="A -> AVA (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 13
FT /note="M -> L (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 157
FT /note="E -> D (in Ref. 1; AAB03675)"
FT /evidence="ECO:0000305"
FT CONFLICT 205
FT /note="G -> S (in Ref. 1; AAB03675)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 213 AA; 25085 MW; 7A56D7948CA0ECDD CRC64;
MHHHQNLGDA AAMNGMIPPY EAMAMYEQPK PRFIFKMPRV VPDQRSKFDS DELFRRLSRE
SEVRYTGYRE RAMEERRMRF VNDCRKGYAE ISMVASGTNL QLYFNANHNP YAQEQDCDFE
RERGKVHLRS SFIMNGVCVR FRGWVDLDRL DGAACLEFDE QRAQQEDAQL QEQIQSYNQR
MAESRRIYHT PQTPPEDHHH RGGPGLPRGP MGW
