THIOT_DROME
ID THIOT_DROME Reviewed; 157 AA.
AC Q8IFW4; Q9W4D6;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Thioredoxin-T;
DE Short=ThioredoxinT;
GN Name=TrxT; ORFNames=CG3315;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Testis;
RX PubMed=14579129; DOI=10.1007/s00412-003-0253-5;
RA Svensson M.J., Chen J.D., Pirrotta V., Larsson J.;
RT "The ThioredoxinT and deadhead gene pair encode testis- and ovary-specific
RT thioredoxins in Drosophila melanogaster.";
RL Chromosoma 112:133-143(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Probably participates in various redox reactions through the
CC reversible oxidation of its active center dithiol to a disulfide and
CC catalyzes dithiol-disulfide exchange reactions. Its tissue specificity
CC suggests a regulatory role in the germline.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14579129}. Chromosome
CC {ECO:0000269|PubMed:14579129}. Note=Specifically associated with the Y
CC chromosome loops.
CC -!- TISSUE SPECIFICITY: Testis specific. Not expressed in the embryo.
CC Becomes progressively more strongly expressed during larval and pupal
CC development. In testis, it is strongly expressed in young
CC spermatocytes, and postmeiotic spermatid stages, then expression
CC decreases at the nuclear elongation stage. Strongly expressed in the
CC waste bag, in which material no longer needed for the mature sperm is
CC eliminated. Not expressed in the stem cells and spermatogonial cells.
CC {ECO:0000269|PubMed:14579129}.
CC -!- DEVELOPMENTAL STAGE: Not expressed maternally.
CC {ECO:0000269|PubMed:14579129}.
CC -!- MISCELLANEOUS: The TrxT gene, which encodes an testis specific
CC thioredoxin, is adjacent to the dhd gene and shares some regulatory
CC region with it.
CC -!- SIMILARITY: Belongs to the thioredoxin family. {ECO:0000305}.
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DR EMBL; AJ507731; CAD45644.1; -; mRNA.
DR EMBL; AE014298; AAF46018.2; -; Genomic_DNA.
DR RefSeq; NP_001284881.1; NM_001297952.1.
DR RefSeq; NP_572212.1; NM_131984.1.
DR PDB; 6Z7O; X-ray; 2.33 A; A=1-157.
DR PDBsum; 6Z7O; -.
DR AlphaFoldDB; Q8IFW4; -.
DR SMR; Q8IFW4; -.
DR BioGRID; 57953; 1.
DR IntAct; Q8IFW4; 18.
DR STRING; 7227.FBpp0070722; -.
DR PaxDb; Q8IFW4; -.
DR DNASU; 31443; -.
DR GeneID; 31443; -.
DR KEGG; dme:Dmel_CG3315; -.
DR CTD; 31443; -.
DR FlyBase; FBgn0029752; TrxT.
DR VEuPathDB; VectorBase:FBgn0029752; -.
DR eggNOG; KOG0907; Eukaryota.
DR HOGENOM; CLU_090389_7_0_1; -.
DR InParanoid; Q8IFW4; -.
DR PhylomeDB; Q8IFW4; -.
DR BioGRID-ORCS; 31443; 0 hits in 3 CRISPR screens.
DR ChiTaRS; TrxT; fly.
DR GenomeRNAi; 31443; -.
DR PRO; PR:Q8IFW4; -.
DR Proteomes; UP000000803; Chromosome X.
DR ExpressionAtlas; Q8IFW4; baseline and differential.
DR Genevisible; Q8IFW4; DM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000806; C:Y chromosome; IDA:UniProtKB.
DR GO; GO:0015036; F:disulfide oxidoreductase activity; ISS:FlyBase.
DR GO; GO:0015035; F:protein-disulfide reductase activity; IEA:InterPro.
DR GO; GO:0006457; P:protein folding; IDA:FlyBase.
DR InterPro; IPR005746; Thioredoxin.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR InterPro; IPR017937; Thioredoxin_CS.
DR InterPro; IPR013766; Thioredoxin_domain.
DR Pfam; PF00085; Thioredoxin; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR TIGRFAMs; TIGR01068; thioredoxin; 1.
DR PROSITE; PS00194; THIOREDOXIN_1; 1.
DR PROSITE; PS51352; THIOREDOXIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chromosome; Disulfide bond; Electron transport; Nucleus;
KW Redox-active center; Reference proteome; Transport.
FT CHAIN 1..157
FT /note="Thioredoxin-T"
FT /id="PRO_0000120036"
FT DOMAIN 2..107
FT /note="Thioredoxin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT REGION 132..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..148
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 32..35
FT /note="Redox-active"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT CONFLICT 47..48
FT /note="HE -> QQ (in Ref. 2; AAF46018)"
FT /evidence="ECO:0000305"
FT HELIX 8..17
FT /evidence="ECO:0007829|PDB:6Z7O"
FT TURN 18..20
FT /evidence="ECO:0007829|PDB:6Z7O"
FT STRAND 21..28
FT /evidence="ECO:0007829|PDB:6Z7O"
FT HELIX 33..48
FT /evidence="ECO:0007829|PDB:6Z7O"
FT TURN 49..52
FT /evidence="ECO:0007829|PDB:6Z7O"
FT STRAND 53..59
FT /evidence="ECO:0007829|PDB:6Z7O"
FT TURN 60..62
FT /evidence="ECO:0007829|PDB:6Z7O"
FT HELIX 64..69
FT /evidence="ECO:0007829|PDB:6Z7O"
FT STRAND 74..82
FT /evidence="ECO:0007829|PDB:6Z7O"
FT STRAND 85..92
FT /evidence="ECO:0007829|PDB:6Z7O"
FT HELIX 95..104
FT /evidence="ECO:0007829|PDB:6Z7O"
SQ SEQUENCE 157 AA; 17499 MW; 5D8EAB28344BFA6B CRC64;
MVYPVRNKDD LDQQLILAED KLVVIDFYAD WCGPCKIIAP KLDELAHEYS DRVVVLKVNV
DENEDITVEY NVNSMPTFVF IKGGNVLELF VGCNSDKLAK LMEKHAGVYT DEAADVKAVH
IDGECIVDLT AESSESDNDN NNVNEVSAHD ENAVLEH
