BRR1_YEAST
ID BRR1_YEAST Reviewed; 341 AA.
AC Q99177; D6W463;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Pre-mRNA-splicing factor BRR1;
DE AltName: Full=Bad response to refrigeration protein 1;
GN Name=BRR1; OrderedLocusNames=YPR057W; ORFNames=YP9499.13;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND ASSOCIATION WITH THE
RP SPLICEOSOME.
RX PubMed=8861964; DOI=10.1002/j.1460-2075.1996.tb00810.x;
RA Noble S.M., Guthrie C.;
RT "Transcriptional pulse-chase analysis reveals a role for a novel snRNP-
RT associated protein in the manufacture of spliceosomal snRNPs.";
RL EMBO J. 15:4368-4379(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION.
RX PubMed=8722763; DOI=10.1093/genetics/143.1.67;
RA Noble S.M., Guthrie C.;
RT "Identification of novel genes required for yeast pre-mRNA splicing by
RT means of cold-sensitive mutations.";
RL Genetics 143:67-80(1996).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION.
RX PubMed=15911574; DOI=10.1534/genetics.105.042044;
RA Brenner T.J., Guthrie C.;
RT "Genetic analysis reveals a role for the C terminus of the Saccharomyces
RT cerevisiae GTPase Snu114 during spliceosome activation.";
RL Genetics 170:1063-1080(2005).
RN [8]
RP INTERACTION WITH HTA1, ASSOCIATION WITH THE SPLICEOSOME, AND IDENTIFICATION
RP BY MASS SPECTROMETRY.
RX PubMed=16429126; DOI=10.1038/nature04532;
RA Gavin A.-C., Aloy P., Grandi P., Krause R., Boesche M., Marzioch M.,
RA Rau C., Jensen L.J., Bastuck S., Duempelfeld B., Edelmann A.,
RA Heurtier M.-A., Hoffman V., Hoefert C., Klein K., Hudak M., Michon A.-M.,
RA Schelder M., Schirle M., Remor M., Rudi T., Hooper S., Bauer A.,
RA Bouwmeester T., Casari G., Drewes G., Neubauer G., Rick J.M., Kuster B.,
RA Bork P., Russell R.B., Superti-Furga G.;
RT "Proteome survey reveals modularity of the yeast cell machinery.";
RL Nature 440:631-636(2006).
CC -!- FUNCTION: Involved in mRNA splicing. Required for snRNA accumulation
CC and manufacture of snRNPs. {ECO:0000269|PubMed:15911574,
CC ECO:0000269|PubMed:8722763, ECO:0000269|PubMed:8861964}.
CC -!- SUBUNIT: Interacts with HTA1 and associates with the spliceosome.
CC {ECO:0000269|PubMed:16429126}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1870 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the BRR1 family. {ECO:0000305}.
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DR EMBL; Z71255; CAA95002.1; -; Genomic_DNA.
DR EMBL; Z49219; CAA89175.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11479.1; -; Genomic_DNA.
DR PIR; S54079; S54079.
DR RefSeq; NP_015382.1; NM_001184154.1.
DR AlphaFoldDB; Q99177; -.
DR SMR; Q99177; -.
DR BioGRID; 36231; 516.
DR DIP; DIP-6711N; -.
DR MINT; Q99177; -.
DR STRING; 4932.YPR057W; -.
DR MaxQB; Q99177; -.
DR PaxDb; Q99177; -.
DR PRIDE; Q99177; -.
DR EnsemblFungi; YPR057W_mRNA; YPR057W; YPR057W.
DR GeneID; 856170; -.
DR KEGG; sce:YPR057W; -.
DR SGD; S000006261; BRR1.
DR VEuPathDB; FungiDB:YPR057W; -.
DR eggNOG; ENOG502S02X; Eukaryota.
DR HOGENOM; CLU_748374_0_0_1; -.
DR InParanoid; Q99177; -.
DR OMA; NEPSHSI; -.
DR BioCyc; YEAST:G3O-34209-MON; -.
DR PRO; PR:Q99177; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q99177; protein.
DR GO; GO:0030532; C:small nuclear ribonucleoprotein complex; IDA:SGD.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0017069; F:snRNA binding; IDA:SGD.
DR GO; GO:0000245; P:spliceosomal complex assembly; IMP:SGD.
DR GO; GO:0000387; P:spliceosomal snRNP assembly; IEA:InterPro.
DR InterPro; IPR023251; Brr1.
DR InterPro; IPR035426; Gemin2/Brr1.
DR Pfam; PF04938; SIP1; 1.
DR PRINTS; PR02039; SPLICEFRBRR1.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Spliceosome.
FT CHAIN 1..341
FT /note="Pre-mRNA-splicing factor BRR1"
FT /id="PRO_0000239636"
SQ SEQUENCE 341 AA; 39860 MW; BFAE093567405283 CRC64;
MKRGESQAPD AIFGQSRAFA LSDSSVNPDV IEYLKSVRQE ALRTNAISIK NHMNLQKRTR
HKSSMYDDED EGALKRHAIS PSLIRLQRNV EIWVRWFNSV KATVLTNAYE FTGYEDETLD
LLLLFLKNYL EDMPSKCTTV EKIISVLNQH SFPEKAEEKE ENLQIDEEWA KNILVRLEKT
KIDSVEDVKK VITEGDKHEL VGYNQWFQYL INNEPQHTTF HEKITSKQLW VLIKYMSNTW
IKEIHKKGRH YRRLQDWLFY ILVHTPERVT AEYTSILRDL GKKCLELIQK KPVEAHENKI
TLPKEMAELN VEIPAAVENM TITELTVSVI AVNYGQKDLI E
