ID   THIO_RHIEC              Reviewed;         327 AA.
AC   O34292; Q2K207;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=Putative thiamine biosynthesis oxidoreductase ThiO;
GN   Name=thiO; OrderedLocusNames=RHE_PB00081;
OS   Rhizobium etli (strain CFN 42 / ATCC 51251).
OG   Plasmid p42b.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=347834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CE3;
RX   PubMed=9371431; DOI=10.1128/jb.179.22.6887-6893.1997;
RA   Miranda-Rios J., Morera C., Taboada H., Davalos A., Encarnacion S.,
RA   Mora J., Soberon M.;
RT   "Expression of thiamin biosynthetic genes (thiCOGE) and production of
RT   symbiotic terminal oxidase cbb3 in Rhizobium etli.";
RL   J. Bacteriol. 179:6887-6893(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFN 42 / ATCC 51251;
RX   PubMed=16505379; DOI=10.1073/pnas.0508502103;
RA   Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.,
RA   Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A.,
RA   Jimenez-Jacinto V., Collado-Vides J., Davila G.;
RT   "The partitioned Rhizobium etli genome: genetic and metabolic redundancy in
RT   seven interacting replicons.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006).
CC   -!- FUNCTION: May have amino acid oxidase activity in the biosynthesis of
CC       thiazole when cysteine gives its thiol group to be inserted into the
CC       thiazole molecule and the rest of the molecule is deaminated to give
CC       pyruvic acid and ammonia.
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis.
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DR   EMBL; AF004408; AAC45973.1; -; Genomic_DNA.
DR   EMBL; CP000135; ABC93123.1; -; Genomic_DNA.
DR   PIR; T44255; T44255.
DR   AlphaFoldDB; O34292; -.
DR   SMR; O34292; -.
DR   EnsemblBacteria; ABC93123; ABC93123; RHE_PB00081.
DR   KEGG; ret:RHE_PB00081; -.
DR   HOGENOM; CLU_007884_1_0_5; -.
DR   OMA; CRRLEPM; -.
DR   UniPathway; UPA00060; -.
DR   Proteomes; UP000001936; Plasmid p42b.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009228; P:thiamine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009229; P:thiamine diphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR012727; Gly_oxidase_ThiO.
DR   Pfam; PF01266; DAO; 1.
DR   TIGRFAMs; TIGR02352; thiamin_ThiO; 1.
PE   4: Predicted;
KW   Oxidoreductase; Plasmid; Reference proteome; Thiamine biosynthesis.
FT   CHAIN           1..327
FT                   /note="Putative thiamine biosynthesis oxidoreductase ThiO"
FT                   /id="PRO_0000072517"
SQ   SEQUENCE   327 AA;  35559 MW;  299B3F74EED3975D CRC64;
     MRILVNGAGV AGLTVAWQLY RHGFRVTLAE RAGTVGAGAS GFAGGMLAPW CERESAEEPV
     LTLGRLAADW WEAALPGHVH RRGTLVVAGG RDTGELDRFS RRTSGWEWLD EVAIAALEPD
     LAGRFRRALF FRQEAHLDPR QALAALAAGL EDARMRLTLG VVGESDVDHD RVVDCTGAAQ
     IGRLPGLRGV RGEMLCVETT EVSLSRPVRL LHPRHPIYIV PRDKNRFMVG ATMIESDDGG
     PITARSLMEL LNAAYAMHPA FGEARVTETG AGVRPAYPDN LPRVTQEGRT LHVNGLYRHG
     FLLAPAMAGE VARRLLTEQG QPERRAS