THIT_LACP3
ID THIT_LACP3 Reviewed; 201 AA.
AC Q037U3;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Thiamine transporter ThiT;
DE AltName: Full=Thiamine ECF transporter S component ThiT;
GN Name=thiT; OrderedLocusNames=LSEI_1757;
OS Lacticaseibacillus paracasei (strain ATCC 334 / BCRC 17002 / CCUG 31169 /
OS CIP 107868 / KCTC 3260 / NRRL B-441) (Lactobacillus paracasei).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lacticaseibacillus.
OX NCBI_TaxID=321967;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 334 / BCRC 17002 / CCUG 31169 / CIP 107868 / KCTC 3260 / NRRL
RC B-441;
RX PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT "Comparative genomics of the lactic acid bacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
RN [2]
RP THIAMINE-BINDING.
RC STRAIN=ATCC 334 / BCRC 17002 / CCUG 31169 / CIP 107868 / KCTC 3260 / NRRL
RC B-441;
RX PubMed=18776013; DOI=10.1128/jb.01070-08;
RA Eudes A., Erkens G.B., Slotboom D.J., Rodionov D.A., Naponelli V.,
RA Hanson A.D.;
RT "Identification of genes encoding the folate- and thiamine-binding membrane
RT proteins in Firmicutes.";
RL J. Bacteriol. 190:7591-7594(2008).
RN [3]
RP FUNCTION AS A TRANSPORT COMPONENT, PROBABLE SUBUNIT, AND EXPRESSION IN
RP L.LACTIS.
RC STRAIN=ATCC 334 / BCRC 17002 / CCUG 31169 / CIP 107868 / KCTC 3260 / NRRL
RC B-441;
RX PubMed=18931129; DOI=10.1128/jb.01208-08;
RA Rodionov D.A., Hebbeln P., Eudes A., ter Beek J., Rodionova I.A.,
RA Erkens G.B., Slotboom D.J., Gelfand M.S., Osterman A.L., Hanson A.D.,
RA Eitinger T.;
RT "A novel class of modular transporters for vitamins in prokaryotes.";
RL J. Bacteriol. 191:42-51(2009).
CC -!- FUNCTION: Thiamine-binding protein that interacts with the energy-
CC coupling factor (ECF) ABC-transporter complex. Unlike classic ABC
CC transporters this ECF transporter provides the energy necessary to
CC transport a number of different substrates. The substrates themselves
CC are bound by transmembrane, not extracytoplasmic soluble proteins and
CC transport it into cells. Binds thiamine with a dissociation constant of
CC 0.5 nM. Upon coexpression with its energy-coupling factor (ECF) ABC-
CC transporter complex EcfA1A2T in Lactococcus lactis subsp. cremoris
CC (strain NZ9000) allows thiamine uptake; uptake requires both ThiT and
CC EcfA1A2T. {ECO:0000269|PubMed:18931129}.
CC -!- SUBUNIT: In Lactococcus lactis subsp. cremoris (strain NZ9000) forms a
CC stable energy-coupling factor (ECF) transporter complex probably
CC composed of a membrane-embedded substrate-binding protein (S
CC component), two ATP-binding proteins (A components) and a transmembrane
CC protein (T component).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the vitamin uptake transporter (VUT/ECF) (TC
CC 2.A.88) family. Thiamine transporter subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000423; ABJ70529.1; -; Genomic_DNA.
DR RefSeq; WP_003576020.1; NC_008526.1.
DR RefSeq; YP_806971.1; NC_008526.1.
DR AlphaFoldDB; Q037U3; -.
DR SMR; Q037U3; -.
DR STRING; 321967.LSEI_1757; -.
DR TCDB; 2.A.88.3.3; the vitamin uptake transporter (vut) family.
DR EnsemblBacteria; ABJ70529; ABJ70529; LSEI_1757.
DR GeneID; 61269952; -.
DR KEGG; lca:LSEI_1757; -.
DR PATRIC; fig|321967.11.peg.1736; -.
DR HOGENOM; CLU_090959_2_0_9; -.
DR OMA; VQVLFDY; -.
DR Proteomes; UP000001651; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015234; F:thiamine transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR012651; Thia_Transptr_ThiT.
DR Pfam; PF09515; Thia_YuaJ; 1.
DR TIGRFAMs; TIGR02357; ECF_ThiT_YuaJ; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..201
FT /note="Thiamine transporter ThiT"
FT /id="PRO_0000409008"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 201 AA; 21249 MW; 768FB636E884C6DE CRC64;
MQQHKQLVVI LETAIIAAFA MALTYIPHTT GVSAIELNYG LIPIAVLAMR RGLVPAAWAG
FVWGILDLIL RGIGGGSVLN PLQGILEYPI AFTLVGLMGL TFASFQKAVR GSEKVKASGY
AFAGIIIGTF AKYFIHFIAG VVFWGAYAPK GTNVWVYSLI VNGGSALFST VLTIVVVGVL
LTVAPQLFVA KDGKSFSTKA A
