ID   THMS1_BOVIN             Reviewed;         641 AA.
AC   A5D789; A6QQ26;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Protein THEMIS;
DE   AltName: Full=Thymocyte-expressed molecule involved in selection;
GN   Name=THEMIS;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   STRAIN=Hereford; TISSUE=Thymus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a central role in late thymocyte development by
CC       controlling both positive and negative T-cell selection. Required to
CC       sustain and/or integrate signals required for proper lineage commitment
CC       and maturation of T-cells. Regulates T-cell development through T-cell
CC       antigen receptor (TCR) signaling and in particular through the
CC       regulation of calcium influx and phosphorylation of Erk.
CC       {ECO:0000250|UniProtKB:Q8BGW0}.
CC   -!- SUBUNIT: Interacts with PLCG1, ITK, GRB2, and LAT.
CC       {ECO:0000250|UniProtKB:Q8BGW0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8BGW0}. Nucleus
CC       {ECO:0000250|UniProtKB:Q8BGW0}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=A5D789-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=A5D789-2; Sequence=VSP_037968;
CC   -!- PTM: Phosphorylated on Tyr residues quickly after TCR stimulation.
CC       {ECO:0000250|UniProtKB:Q8BGW0}.
CC   -!- SIMILARITY: Belongs to the themis family. {ECO:0000305}.
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DR   EMBL; BC140469; AAI40470.1; -; mRNA.
DR   EMBL; BC149610; AAI49611.1; -; mRNA.
DR   RefSeq; NP_001091585.1; NM_001098116.1. [A5D789-1]
DR   AlphaFoldDB; A5D789; -.
DR   STRING; 9913.ENSBTAP00000020742; -.
DR   PaxDb; A5D789; -.
DR   PRIDE; A5D789; -.
DR   Ensembl; ENSBTAT00000020742; ENSBTAP00000020742; ENSBTAG00000015615. [A5D789-1]
DR   GeneID; 540554; -.
DR   KEGG; bta:540554; -.
DR   CTD; 387357; -.
DR   VEuPathDB; HostDB:ENSBTAG00000015615; -.
DR   eggNOG; ENOG502QSJR; Eukaryota.
DR   GeneTree; ENSGT00530000063770; -.
DR   HOGENOM; CLU_022319_1_0_1; -.
DR   InParanoid; A5D789; -.
DR   OMA; KVPVGCQ; -.
DR   OrthoDB; 337909at2759; -.
DR   TreeFam; TF333479; -.
DR   Proteomes; UP000009136; Chromosome 9.
DR   Bgee; ENSBTAG00000015615; Expressed in thymus and 39 other tissues.
DR   GO; GO:0005911; C:cell-cell junction; IEA:Ensembl.
DR   GO; GO:0008180; C:COP9 signalosome; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR   GO; GO:0043383; P:negative T cell selection; ISS:UniProtKB.
DR   GO; GO:0043368; P:positive T cell selection; ISS:UniProtKB.
DR   GO; GO:0050852; P:T cell receptor signaling pathway; ISS:UniProtKB.
DR   InterPro; IPR025946; CABIT_dom.
DR   InterPro; IPR039671; THEMIS.
DR   PANTHER; PTHR15215; PTHR15215; 1.
DR   Pfam; PF12736; CABIT; 2.
PE   2: Evidence at transcript level;
KW   Adaptive immunity; Alternative splicing; Cytoplasm; Developmental protein;
KW   Immunity; Nucleus; Phosphoprotein; Reference proteome.
FT   CHAIN           1..641
FT                   /note="Protein THEMIS"
FT                   /id="PRO_0000383150"
FT   REGION          1..259
FT                   /note="CABIT 1"
FT   REGION          260..518
FT                   /note="CABIT 2"
FT   MOD_RES         584
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8N1K5"
FT   VAR_SEQ         133..237
FT                   /note="GERIMLNSVEEINGEIMVNCSVLRNHQNHSFTLPLSQEGEFYECEDEHIYTL
FT                   KEIIQWKIPKNRTRTVILTGFSAKWDSTNPFPRGFYGAVILKPVYEIQAVMKF -> V
FT                   (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_037968"
FT   CONFLICT        468
FT                   /note="I -> V (in Ref. 1; AAI49611)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   641 AA;  73132 MW;  839FB9E6275916E2 CRC64;
     MALSLEEFIH SLDLRTLPRV LEIQSGFYFE GSIYEMFGNE CCLSTGEVIK ITDLKIKKIM
     AEICEHVEGC VSPQSFELPM NFPGLFKIVA DTTPYLTMEE ITKANHIRPS RPNHPCFYHQ
     KDIKLENLII KKGERIMLNS VEEINGEIMV NCSVLRNHQN HSFTLPLSQE GEFYECEDEH
     IYTLKEIIQW KIPKNRTRTV ILTGFSAKWD STNPFPRGFY GAVILKPVYE IQAVMKFRKD
     IVRILPSLDV EVKDITDSYD ANWFLQVLST QDLYEMANKQ FPIVAEVIEA PQGNQLPISI
     LQPGKTIVIH KKCQASKILA SEIRSNSSKR HFLIPTSYKG KFKRRPREFP TAYDLEIAKS
     EKEPLHVVAT KAFHSPFEEL SSVSVGDEFL VHHSQTTEVL CEGIKKVMDG LACEKILKNS
     REVALLPLYM DGGFVEVIHD KKLYQISELC AQFRLPFNVK VSVRDLFIEK DILAAIPGLQ
     LEESITNSYL LISDLANPKK CWEIPVGRVN MTVQLVNNFS GDTGSFLVST LVEEITEEQY
     YLIRRYEKSF LHPPPRPPKH PSVEKIELTP LSLAKQKTVN LPKSPKSVHV DRSKKLYSNQ
     AGLDSKVPAG CQNDLADLEK EKRKTEATAV TEIFKTEEHK K