THMS1_DANRE
ID THMS1_DANRE Reviewed; 675 AA.
AC A5PF62; A4IFY3; Q08B89;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Protein THEMIS;
DE AltName: Full=Thymocyte-expressed molecule involved in selection;
GN Name=themis; ORFNames=ch211-205n18.3, im:7138580;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays a central role in late thymocyte development by
CC controlling both positive and negative T-cell selection. Required to
CC sustain and/or integrate signals required for proper lineage commitment
CC and maturation of T-cells. Regulates T-cell development through T-cell
CC antigen receptor (TCR) signaling and in particular through the
CC regulation of calcium influx and phosphorylation of Erk (By
CC similarity). {ECO:0000250|UniProtKB:Q8BGW0}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8BGW0}. Nucleus
CC {ECO:0000250|UniProtKB:Q8BGW0}.
CC -!- SIMILARITY: Belongs to the themis family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI24828.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAI34837.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL954182; CAN87895.1; -; Genomic_DNA.
DR EMBL; BC124827; AAI24828.1; ALT_INIT; mRNA.
DR EMBL; BC134836; AAI34837.1; ALT_INIT; mRNA.
DR RefSeq; NP_001333171.1; NM_001346242.1.
DR AlphaFoldDB; A5PF62; -.
DR STRING; 7955.ENSDARP00000012705; -.
DR PaxDb; A5PF62; -.
DR Ensembl; ENSDART00000002928; ENSDARP00000012705; ENSDARG00000010619.
DR GeneID; 555433; -.
DR KEGG; dre:555433; -.
DR CTD; 387357; -.
DR ZFIN; ZDB-GENE-041008-160; themis.
DR eggNOG; ENOG502QSJR; Eukaryota.
DR GeneTree; ENSGT00530000063770; -.
DR HOGENOM; CLU_022319_1_0_1; -.
DR InParanoid; A5PF62; -.
DR OMA; KVPVGCQ; -.
DR OrthoDB; 337909at2759; -.
DR PhylomeDB; A5PF62; -.
DR TreeFam; TF333479; -.
DR PRO; PR:A5PF62; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 20.
DR Bgee; ENSDARG00000010619; Expressed in intestine and 10 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR GO; GO:0043383; P:negative T cell selection; ISS:UniProtKB.
DR GO; GO:0043368; P:positive T cell selection; ISS:UniProtKB.
DR GO; GO:0050852; P:T cell receptor signaling pathway; ISS:UniProtKB.
DR InterPro; IPR025946; CABIT_dom.
DR InterPro; IPR039671; THEMIS.
DR PANTHER; PTHR15215; PTHR15215; 1.
DR Pfam; PF12736; CABIT; 2.
PE 2: Evidence at transcript level;
KW Adaptive immunity; Cytoplasm; Developmental protein; Immunity; Nucleus;
KW Reference proteome.
FT CHAIN 1..675
FT /note="Protein THEMIS"
FT /id="PRO_0000383151"
FT REGION 1..252
FT /note="CABIT 1"
FT REGION 253..510
FT /note="CABIT 2"
FT REGION 543..582
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 594..643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 564..581
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 621..643
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 6
FT /note="Q -> E (in Ref. 2; AAI34837/AAI24828)"
FT /evidence="ECO:0000305"
FT CONFLICT 82
FT /note="M -> V (in Ref. 2; AAI34837/AAI24828)"
FT /evidence="ECO:0000305"
FT CONFLICT 535
FT /note="I -> L (in Ref. 2; AAI34837/AAI24828)"
FT /evidence="ECO:0000305"
FT CONFLICT 595
FT /note="C -> S (in Ref. 2; AAI34837/AAI24828)"
FT /evidence="ECO:0000305"
FT CONFLICT 600
FT /note="G -> V (in Ref. 2; AAI34837/AAI24828)"
FT /evidence="ECO:0000305"
FT CONFLICT 607
FT /note="I -> V (in Ref. 2; AAI34837/AAI24828)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 675 AA; 76625 MW; 939DA39457CDD6EE CRC64;
MAQTLQEFTH ALEEKHLPRV LHIQSGIYYQ GCVYEVFGQE CSLSTGDLLK VTEITISRFN
ALTSSSTEIQ LPVEYPGLFK LMADSQPYRS IQEIADSLKI GPQRVAQPVF LSAVDVDLEP
GLVLRRRDSF RITAVEWSCG RVHCELLHRE PPICFSLSFC QQGPFSECQD HQCYTLKEIA
DWKIPRGRKR SVVEVKAPAK KNFLFSGLLE NVCGELLLTP VYELKAISRL SEKVLLIPSN
LDVEVVDVTE QYDCDSFVQP LSLMDVYKRP PQFFPVLAKL SSENHFRLSP DLESLFQSKE
VIIHHPFKAK RILASEMCQE SARHFLIPES YNGRFKRRPR QFSTAYDLER ARSETEEIRV
VATKDFETVY SGLASVQAGE EFIVTKGQSC AVSHNGTEKV ADTFECVKVK AEGKEPVRLP
MCLEGGFMEL VKDKRQYTIA EICRWFPLPF NVKVSVRDLS LKADILAGTP SLHIEEEISD
PCVLVSNTDL SDFREVPVNR TDLIFNVKQR WDGESPKFSV KSAIEEISED CYYTIRRYAI
ATITPPPRPP KKPKDPPPRP PKTSRSESTN SESSSCSPKT LHVEPFKQDG LLQCCDSRNG
NEQKIPISPV TLPRPSLQKA RAKGRSLDDI SMDKHAASDN DVHDYEYIDE EQLDNIRRTY
QEQQINTKAK PSHTI
