ID   THN22_ARATH             Reviewed;         134 AA.
AC   Q42597; Q8LEF0; Q9FIW2;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 2.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Thionin-2.2;
DE   Contains:
DE     RecName: Full=Thionin-2.2;
DE   Contains:
DE     RecName: Full=Acidic protein;
DE   Flags: Precursor;
GN   Name=THI2.2; OrderedLocusNames=At5g36910; ORFNames=MLF18.5;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=8552715; DOI=10.1104/pp.109.3.813;
RA   Epple P., Apel K., Bohlmann H.;
RT   "An Arabidopsis thaliana thionin gene is inducible via a signal
RT   transduction pathway different from that for pathogenesis-related
RT   proteins.";
RL   Plant Physiol. 109:813-820(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA   Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT   features of the regions of 1,081,958 bp covered by seventeen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:379-391(1998).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Thionins are small plant proteins which are toxic to animal
CC       cells. They seem to exert their toxic effect at the level of the cell
CC       membrane. Their precise function is not known.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Low basal expression in seedlings. Also detected in
CC       rosette leaves. {ECO:0000269|PubMed:8552715}.
CC   -!- SIMILARITY: Belongs to the plant thionin (TC 1.C.44) family.
CC       {ECO:0000305}.
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DR   EMBL; L41245; AAC41679.1; -; mRNA.
DR   EMBL; AB016877; BAB11632.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED94125.1; -; Genomic_DNA.
DR   EMBL; AY085455; AAM62681.1; -; mRNA.
DR   RefSeq; NP_198507.1; NM_123049.3.
DR   AlphaFoldDB; Q42597; -.
DR   SMR; Q42597; -.
DR   STRING; 3702.AT5G36910.1; -.
DR   PaxDb; Q42597; -.
DR   PRIDE; Q42597; -.
DR   EnsemblPlants; AT5G36910.1; AT5G36910.1; AT5G36910.
DR   GeneID; 833659; -.
DR   Gramene; AT5G36910.1; AT5G36910.1; AT5G36910.
DR   KEGG; ath:AT5G36910; -.
DR   Araport; AT5G36910; -.
DR   TAIR; locus:2167504; AT5G36910.
DR   eggNOG; ENOG502SUEZ; Eukaryota.
DR   HOGENOM; CLU_132328_0_0_1; -.
DR   InParanoid; Q42597; -.
DR   OMA; STMNTCA; -.
DR   OrthoDB; 1491167at2759; -.
DR   PhylomeDB; Q42597; -.
DR   PRO; PR:Q42597; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q42597; baseline and differential.
DR   Genevisible; Q42597; AT.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; ISS:TAIR.
DR   Gene3D; 3.30.1350.10; -; 1.
DR   InterPro; IPR001010; Thionin.
DR   InterPro; IPR036391; Thionin-like_sf.
DR   PANTHER; PTHR33920; PTHR33920; 1.
DR   Pfam; PF00321; Thionin; 1.
DR   SUPFAM; SSF57429; SSF57429; 1.
DR   PROSITE; PS00271; THIONIN; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Plant defense; Reference proteome; Secreted; Signal; Toxin.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..70
FT                   /note="Thionin-2.2"
FT                   /id="PRO_0000034141"
FT   CHAIN           71..134
FT                   /note="Acidic protein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000034142"
FT   DISULFID        27..64
FT                   /evidence="ECO:0000250"
FT   DISULFID        28..56
FT                   /evidence="ECO:0000250"
FT   DISULFID        40..50
FT                   /evidence="ECO:0000250"
FT   CONFLICT        13
FT                   /note="M -> I (in Ref. 4; AAM62681)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        104
FT                   /note="T -> A (in Ref. 1; AAC41679 and 4; AAM62681)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   134 AA;  14178 MW;  5F8A160F22E97CF4 CRC64;
     MEGKTVISSL LIMSLVLAQI QVEAKICCPT KDDRSVYFVC MLSVSSQFYC LLKSKCKNTS
     QTICPPGYTN DILENSGDAV NEYCKLGCAS SVCGALTTLQ NFDTSKVLSE AVEQCTKACS
     SVCTGGSTAA VKSA