THN23_ARATH
ID THN23_ARATH Reviewed; 135 AA.
AC Q8VZK8; Q56XM5; Q6AWT2; Q9ZUL0;
DT 01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Probable thionin-2.3;
DE Contains:
DE RecName: Full=Probable thionin-2.3;
DE Contains:
DE RecName: Full=Acidic protein;
DE Flags: Precursor;
GN OrderedLocusNames=At2g15010; ORFNames=T15J14.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Thionins are small plant proteins which are toxic to animal
CC cells. They seem to exert their toxic effect at the level of the cell
CC membrane. Their precise function is not known.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the plant thionin (TC 1.C.44) family.
CC {ECO:0000305}.
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DR EMBL; AC005957; AAD03358.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06359.1; -; Genomic_DNA.
DR EMBL; AY064042; AAL36398.1; -; mRNA.
DR EMBL; BT015166; AAT85762.1; -; mRNA.
DR EMBL; AK221648; BAD95310.1; -; mRNA.
DR PIR; H84523; H84523.
DR RefSeq; NP_179105.1; NM_127063.5.
DR AlphaFoldDB; Q8VZK8; -.
DR SMR; Q8VZK8; -.
DR STRING; 3702.AT2G15010.1; -.
DR PaxDb; Q8VZK8; -.
DR PRIDE; Q8VZK8; -.
DR ProteomicsDB; 234350; -.
DR EnsemblPlants; AT2G15010.1; AT2G15010.1; AT2G15010.
DR GeneID; 815990; -.
DR Gramene; AT2G15010.1; AT2G15010.1; AT2G15010.
DR KEGG; ath:AT2G15010; -.
DR Araport; AT2G15010; -.
DR TAIR; locus:2055797; AT2G15010.
DR HOGENOM; CLU_132328_0_0_1; -.
DR InParanoid; Q8VZK8; -.
DR OMA; CAKLSTC; -.
DR OrthoDB; 1491167at2759; -.
DR PhylomeDB; Q8VZK8; -.
DR PRO; PR:Q8VZK8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8VZK8; baseline and differential.
DR Genevisible; Q8VZK8; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; ISS:TAIR.
DR Gene3D; 3.30.1350.10; -; 1.
DR InterPro; IPR001010; Thionin.
DR InterPro; IPR036391; Thionin-like_sf.
DR PANTHER; PTHR33920; PTHR33920; 1.
DR Pfam; PF00321; Thionin; 1.
DR SUPFAM; SSF57429; SSF57429; 1.
DR PROSITE; PS00271; THIONIN; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Plant defense; Reference proteome; Secreted; Signal; Toxin.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..71
FT /note="Probable thionin-2.3"
FT /id="PRO_0000034143"
FT CHAIN 72..135
FT /note="Acidic protein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000034144"
FT DISULFID 28..65
FT /evidence="ECO:0000250"
FT DISULFID 29..57
FT /evidence="ECO:0000250"
FT DISULFID 41..51
FT /evidence="ECO:0000250"
FT CONFLICT 37
FT /note="E -> G (in Ref. 3; AAL36398 and 5; BAD95310)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 135 AA; 14259 MW; 162E0ED270038921 CRC64;
MEGKTVIFSV LVMGLVISQI QVEAQKTCCP SQSTRKEFED CISEGNLQIL CSAESGCRDT
YVGYCPSGFP YGSLTNSGDV VNVYCKLGCV SSLCGALTSL QKLDTSGKVN VAVERCTKAC
STICTKGSKT AVETV
