ID   THN2_VISAL              Reviewed;          46 AA.
AC   P32880; P01536;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Viscotoxin-A2;
GN   Name=THI2.3;
OS   Viscum album (European mistletoe).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Santalales; Viscaceae; Viscum.
OX   NCBI_TaxID=3972;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=5035954; DOI=10.3891/acta.chem.scand.26-0585;
RA   Olson T., Samuelsson G.;
RT   "The amino acid sequence of viscotoxin A2 from the European mistletoe
RT   (Viscum album L., Loranthaceae).";
RL   Acta Chem. Scand. 26:585-595(1972).
RN   [2]
RP   DISULFIDE BONDS.
RX   PubMed=4607177;
RA   Olson T., Samuelsson G.;
RT   "The disulphide bonds of viscotoxin A2 from the European mistletoe (Viscum
RT   album L. Loranthaceae).";
RL   Acta Pharm. Suec. 11:381-386(1974).
RN   [3]
RP   STRUCTURE BY NMR OF 1-42, AND DISULFIDE BONDS.
RX   PubMed=12733989; DOI=10.1042/bj20030488;
RA   Coulon A., Mosbah A., Lopez A., Sautereau A.-M., Schaller G., Urech K.,
RA   Rouge P., Darbon H.;
RT   "Comparative membrane interaction study of viscotoxins A3, A2 and B from
RT   mistletoe (Viscum album) and connections with their structures.";
RL   Biochem. J. 374:71-78(2003).
CC   -!- FUNCTION: Thionins are small plant proteins which are toxic to animal
CC       cells. They seem to exert their toxic effect at the level of the cell
CC       membrane. Their precise function is not known.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the plant thionin (TC 1.C.44) family.
CC       {ECO:0000305}.
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DR   PIR; A90005; VTVAA2.
DR   PDB; 1JMN; NMR; -; A=1-44.
DR   PDBsum; 1JMN; -.
DR   AlphaFoldDB; P32880; -.
DR   SMR; P32880; -.
DR   EvolutionaryTrace; P32880; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1350.10; -; 1.
DR   InterPro; IPR001010; Thionin.
DR   InterPro; IPR036391; Thionin-like_sf.
DR   PANTHER; PTHR33920; PTHR33920; 1.
DR   Pfam; PF00321; Thionin; 1.
DR   PRINTS; PR00287; THIONIN.
DR   SUPFAM; SSF57429; SSF57429; 1.
DR   PROSITE; PS00271; THIONIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Plant defense;
KW   Secreted; Toxin.
FT   CHAIN           1..46
FT                   /note="Viscotoxin-A2"
FT                   /id="PRO_0000221483"
FT   DISULFID        3..40
FT   DISULFID        4..32
FT   DISULFID        16..26
FT                   /evidence="ECO:0000269|PubMed:4607177"
FT   HELIX           7..17
FT                   /evidence="ECO:0007829|PDB:1JMN"
FT   TURN            18..20
FT                   /evidence="ECO:0007829|PDB:1JMN"
FT   HELIX           24..30
FT                   /evidence="ECO:0007829|PDB:1JMN"
FT   STRAND          36..38
FT                   /evidence="ECO:0007829|PDB:1JMN"
SQ   SEQUENCE   46 AA;  4834 MW;  61149207EE3FF0E5 CRC64;
     KSCCPNTTGR NIYNTCRFGG GSREVCASLS GCKIISASTC PSYPDK