ID   THN2_WHEAT              Reviewed;         136 AA.
AC   P32032;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Alpha-2-purothionin;
DE   Contains:
DE     RecName: Full=Alpha-2-purothionin;
DE   Contains:
DE     RecName: Full=Acidic protein;
DE   Flags: Precursor;
GN   Name=THI1.2; Synonyms=PUR-B1;
OS   Triticum aestivum (Wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4565;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Endosperm;
RX   PubMed=7824649; DOI=10.1104/pp.106.3.1221;
RA   Castagnaro A., Marana C., Carbonero P., Garcia-Olmedo F.;
RT   "cDNA cloning and nucleotide sequences of alpha 1 and alpha 2 thionins from
RT   hexaploid wheat endosperm.";
RL   Plant Physiol. 106:1221-1222(1994).
CC   -!- FUNCTION: Thionins are small plant proteins which are toxic to animal
CC       cells. They seem to exert their toxic effect at the level of the cell
CC       membrane. Their precise function is not known.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the plant thionin (TC 1.C.44) family.
CC       {ECO:0000305}.
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DR   EMBL; X70665; CAA50003.1; -; mRNA.
DR   PIR; S31695; S31695.
DR   AlphaFoldDB; P32032; -.
DR   SMR; P32032; -.
DR   STRING; 4565.Traes_1DL_AB9D8EBCA.1; -.
DR   Allergome; 9834; Tri a 37.
DR   PRIDE; P32032; -.
DR   EnsemblPlants; TraesCAD_scaffold_059422_01G000500.1; TraesCAD_scaffold_059422_01G000500.1; TraesCAD_scaffold_059422_01G000500.
DR   EnsemblPlants; TraesCLE_scaffold_055580_01G000600.1; TraesCLE_scaffold_055580_01G000600.1; TraesCLE_scaffold_055580_01G000600.
DR   EnsemblPlants; TraesCS1D02G405700.1; TraesCS1D02G405700.1; TraesCS1D02G405700.
DR   EnsemblPlants; TraesPAR_scaffold_055909_01G000200.1; TraesPAR_scaffold_055909_01G000200.1; TraesPAR_scaffold_055909_01G000200.
DR   EnsemblPlants; TraesROB_scaffold_057461_01G000600.1; TraesROB_scaffold_057461_01G000600.1; TraesROB_scaffold_057461_01G000600.
DR   EnsemblPlants; TraesWEE_scaffold_055449_01G000200.1; TraesWEE_scaffold_055449_01G000200.1; TraesWEE_scaffold_055449_01G000200.
DR   Gramene; TraesCAD_scaffold_059422_01G000500.1; TraesCAD_scaffold_059422_01G000500.1; TraesCAD_scaffold_059422_01G000500.
DR   Gramene; TraesCLE_scaffold_055580_01G000600.1; TraesCLE_scaffold_055580_01G000600.1; TraesCLE_scaffold_055580_01G000600.
DR   Gramene; TraesCS1D02G405700.1; TraesCS1D02G405700.1; TraesCS1D02G405700.
DR   Gramene; TraesPAR_scaffold_055909_01G000200.1; TraesPAR_scaffold_055909_01G000200.1; TraesPAR_scaffold_055909_01G000200.
DR   Gramene; TraesROB_scaffold_057461_01G000600.1; TraesROB_scaffold_057461_01G000600.1; TraesROB_scaffold_057461_01G000600.
DR   Gramene; TraesWEE_scaffold_055449_01G000200.1; TraesWEE_scaffold_055449_01G000200.1; TraesWEE_scaffold_055449_01G000200.
DR   eggNOG; ENOG502R3I9; Eukaryota.
DR   HOGENOM; CLU_132328_0_0_1; -.
DR   OMA; VENCGDA; -.
DR   Proteomes; UP000019116; Unplaced.
DR   ExpressionAtlas; P32032; baseline.
DR   Genevisible; P32032; TA.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1350.10; -; 1.
DR   InterPro; IPR001010; Thionin.
DR   InterPro; IPR036391; Thionin-like_sf.
DR   PANTHER; PTHR33920; PTHR33920; 1.
DR   Pfam; PF00321; Thionin; 1.
DR   PRINTS; PR00287; THIONIN.
DR   SUPFAM; SSF57429; SSF57429; 1.
DR   PROSITE; PS00271; THIONIN; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Plant defense; Reference proteome; Secreted; Signal; Toxin.
FT   SIGNAL          1..27
FT   CHAIN           28..72
FT                   /note="Alpha-2-purothionin"
FT                   /id="PRO_0000034128"
FT   CHAIN           73..136
FT                   /note="Acidic protein"
FT                   /id="PRO_0000034129"
FT   DISULFID        30..66
FT                   /evidence="ECO:0000250"
FT   DISULFID        31..58
FT                   /evidence="ECO:0000250"
FT   DISULFID        39..56
FT                   /evidence="ECO:0000250"
FT   DISULFID        43..52
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   136 AA;  14558 MW;  B4019F014E226B9F CRC64;
     MGSKGLKGVM VCLLILGLVL EQVQVEGKSC CRTTLGRNCY NLCRSRGAQK LCSTVCRCKL
     TSGLSCPKGF PKLALESNSD EPDTIEYCNL GCRSSVCDYM VNAAADDEEM KLYVENCGDA
     CVNFCNGDAG LTSLDA