ID   THN3_HORVU              Reviewed;         137 AA.
AC   P08772;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Leaf-specific thionin DB4;
DE   Contains:
DE     RecName: Full=Leaf-specific thionin DB4;
DE   Contains:
DE     RecName: Full=Acidic protein;
DE   Flags: Precursor;
GN   Name=THI1.3;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Carina;
RA   Bohlmann H., Apel K.;
RT   "Isolation and characterization of cDNAs coding for leaf-specific thionins
RT   closely related to the endosperm-specific hordothionin of barley (Hordeum
RT   vulgare L.).";
RL   Mol. Gen. Genet. 207:446-454(1987).
CC   -!- FUNCTION: Thionins are small plant proteins which are toxic to animal
CC       cells. They seem to exert their toxic effect at the level of the cell
CC       membrane. Their precise function is not known.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the plant thionin (TC 1.C.44) family.
CC       {ECO:0000305}.
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DR   EMBL; X05576; CAA29082.1; -; mRNA.
DR   PIR; S07648; S07648.
DR   AlphaFoldDB; P08772; -.
DR   SMR; P08772; -.
DR   ExpressionAtlas; P08772; baseline.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1350.10; -; 1.
DR   InterPro; IPR001010; Thionin.
DR   InterPro; IPR036391; Thionin-like_sf.
DR   PANTHER; PTHR33920; PTHR33920; 1.
DR   Pfam; PF00321; Thionin; 1.
DR   PRINTS; PR00287; THIONIN.
DR   SUPFAM; SSF57429; SSF57429; 1.
DR   PROSITE; PS00271; THIONIN; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Plant defense; Secreted; Signal; Toxin.
FT   SIGNAL          1..28
FT   CHAIN           29..74
FT                   /note="Leaf-specific thionin DB4"
FT                   /id="PRO_0000034116"
FT   CHAIN           75..137
FT                   /note="Acidic protein"
FT                   /id="PRO_0000034117"
FT   DISULFID        31..68
FT                   /evidence="ECO:0000250"
FT   DISULFID        32..60
FT                   /evidence="ECO:0000250"
FT   DISULFID        40..58
FT                   /evidence="ECO:0000250"
FT   DISULFID        44..54
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   137 AA;  14711 MW;  5E76AECB4EE12DE7 CRC64;
     MAPSKSIKSV VICVLILGLV LEQVQVEGKS CCKDTLARNC YNTCHFAGGS RPVCAGACRC
     KIISGPKCPS DYPKLNLLPE SGEPDVTQYC TIGCRNSVCD NMDNVFRGQE MKFDMGLCSN
     ACARFCNDGA VIQSVEA