ID   THN5_HORVU              Reviewed;         137 AA.
AC   P09617;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 2.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Leaf-specific thionin;
DE   Contains:
DE     RecName: Full=Leaf-specific thionin;
DE   Contains:
DE     RecName: Full=Acidic protein;
DE   Flags: Precursor;
GN   Name=THI1.5;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Gausing K.;
RT   "Thionin genes specifically expressed in barley leaves.";
RL   Planta 171:241-246(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Carina;
RX   PubMed=1377959; DOI=10.1007/bf00027341;
RA   Andresen I., Becker W., Schluter K., Burges J., Parthier B., Apel K.;
RT   "The identification of leaf thionin as one of the main jasmonate-induced
RT   proteins of barley (Hordeum vulgare).";
RL   Plant Mol. Biol. 19:193-204(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 29-74.
RC   STRAIN=cv. Carina;
RX   PubMed=16453847; DOI=10.1002/j.1460-2075.1988.tb02980.x;
RA   Bohlmann H., Clausen S., Behnke S., Giese H., Hiller C.,
RA   Reimann-Phillip U., Schrader G., Barkholt V., Apel K.;
RT   "Leaf-specific thionins of barley - a novel class of cell wall proteins
RT   toxic to plant-pathogenic fungi and possibly involved in the defence
RT   mechanism of plants.";
RL   EMBO J. 7:1559-1565(1988).
CC   -!- FUNCTION: Thionins are small plant proteins which are toxic to animal
CC       cells. They seem to exert their toxic effect at the level of the cell
CC       membrane. Their precise function is not known.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the plant thionin (TC 1.C.44) family.
CC       {ECO:0000305}.
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DR   EMBL; M19046; AAA32976.1; -; mRNA.
DR   EMBL; M19047; AAA32977.1; -; mRNA.
DR   EMBL; M19048; AAA32978.1; -; mRNA.
DR   PIR; S22515; S22515.
DR   AlphaFoldDB; P09617; -.
DR   SMR; P09617; -.
DR   ExpressionAtlas; P09617; baseline.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1350.10; -; 1.
DR   InterPro; IPR001010; Thionin.
DR   InterPro; IPR036391; Thionin-like_sf.
DR   PANTHER; PTHR33920; PTHR33920; 1.
DR   Pfam; PF00321; Thionin; 1.
DR   PRINTS; PR00287; THIONIN.
DR   SUPFAM; SSF57429; SSF57429; 1.
DR   PROSITE; PS00271; THIONIN; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Plant defense; Secreted; Signal; Toxin.
FT   SIGNAL          1..28
FT   CHAIN           29..74
FT                   /note="Leaf-specific thionin"
FT                   /id="PRO_0000034118"
FT   CHAIN           75..137
FT                   /note="Acidic protein"
FT                   /id="PRO_0000034119"
FT   DISULFID        31..68
FT                   /evidence="ECO:0000250"
FT   DISULFID        32..60
FT                   /evidence="ECO:0000250"
FT   DISULFID        40..58
FT                   /evidence="ECO:0000250"
FT   DISULFID        44..54
FT                   /evidence="ECO:0000250"
FT   CONFLICT        28
FT                   /note="A -> G (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        135
FT                   /note="V -> L (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   137 AA;  14662 MW;  FD00C2DBF6632797 CRC64;
     MATNKSIKSV VICVLILGLV LEQVQVEAKS CCKNTTGRNC YNACRFAGGS RPVCATACGC
     KIISGPTCPR DYPKLNLLPE SGEPNATEYC TIGCRTSVCD NMDNVSRGQE MKFDMGLCSN
     ACARFCNDGE VIQSVEA