ID   THNA_HORVU              Reviewed;         127 AA.
AC   P01545;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-1986, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Alpha-hordothionin;
DE   AltName: Full=Purothionin II;
DE   Contains:
DE     RecName: Full=Alpha-hordothionin;
DE   Contains:
DE     RecName: Full=Acidic protein;
DE   Flags: Precursor;
GN   Name=THI1.1;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=3082629; DOI=10.1111/j.1432-1033.1986.tb09557.x;
RA   Ponz F., Paz-Ares J., Hernandez-Lucas C., Garcia-Olmedo F., Carbonero P.;
RT   "Cloning and nucleotide sequence of a cDNA encoding the precursor of the
RT   barley toxin alpha-hordothionin.";
RL   Eur. J. Biochem. 156:131-135(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2850969; DOI=10.1016/0378-1119(88)90199-0;
RA   Rodriguez-Palenzuela P., Pintor-Toro J.A., Carbonero P., Garcia-Olmedo F.;
RT   "Nucleotide sequence and endosperm-specific expression of the structural
RT   gene for the toxin alpha-hordothionin in barley (Hordeum vulgare L.).";
RL   Gene 70:271-281(1988).
RN   [3]
RP   PROTEIN SEQUENCE OF 19-63.
RX   PubMed=6987216; DOI=10.1093/oxfordjournals.jbchem.a132777;
RA   Ozaki Y., Wada K., Hase T., Matsubara H., Nakanishi T., Yoshizumi H.;
RT   "Amino acid sequence of a purothionin homolog from barley flour.";
RL   J. Biochem. 87:549-555(1980).
RN   [4]
RP   PROTEIN SEQUENCE OF 19-27.
RC   STRAIN=cv. Bomi; TISSUE=Starchy endosperm;
RX   PubMed=11271488;
RX   DOI=10.1002/1522-2683(200011)21:17<3693::aid-elps3693>3.0.co;2-i;
RA   Kristoffersen H.E., Flengsrud R.;
RT   "Separation and characterization of basic barley seed proteins.";
RL   Electrophoresis 21:3693-3700(2000).
CC   -!- FUNCTION: Thionins are small plant proteins which are toxic to animal
CC       cells. They seem to exert their toxic effect at the level of the cell
CC       membrane. Their precise function is not known.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the plant thionin (TC 1.C.44) family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA32966.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X05901; CAA29330.1; -; mRNA.
DR   EMBL; M23080; AAA32966.1; ALT_INIT; Genomic_DNA.
DR   PIR; JA0087; VSBH2.
DR   AlphaFoldDB; P01545; -.
DR   SMR; P01545; -.
DR   EnsemblPlants; HORVU.MOREX.r2.1HG0072570.1; HORVU.MOREX.r2.1HG0072570.1; HORVU.MOREX.r2.1HG0072570.
DR   EnsemblPlants; HORVU.MOREX.r2.1HG0072590.1.mrna1; HORVU.MOREX.r2.1HG0072590.1.mrna1; HORVU.MOREX.r2.1HG0072590.1.
DR   Gramene; HORVU.MOREX.r2.1HG0072570.1; HORVU.MOREX.r2.1HG0072570.1; HORVU.MOREX.r2.1HG0072570.
DR   Gramene; HORVU.MOREX.r2.1HG0072590.1.mrna1; HORVU.MOREX.r2.1HG0072590.1.mrna1; HORVU.MOREX.r2.1HG0072590.1.
DR   ExpressionAtlas; P01545; baseline.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1350.10; -; 1.
DR   InterPro; IPR001010; Thionin.
DR   InterPro; IPR036391; Thionin-like_sf.
DR   PANTHER; PTHR33920; PTHR33920; 1.
DR   Pfam; PF00321; Thionin; 1.
DR   PRINTS; PR00287; THIONIN.
DR   SUPFAM; SSF57429; SSF57429; 1.
DR   PROSITE; PS00271; THIONIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Plant defense; Secreted; Signal;
KW   Toxin.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000269|PubMed:11271488,
FT                   ECO:0000269|PubMed:6987216"
FT   CHAIN           19..63
FT                   /note="Alpha-hordothionin"
FT                   /id="PRO_0000034112"
FT   CHAIN           64..127
FT                   /note="Acidic protein"
FT                   /id="PRO_0000034113"
FT   DISULFID        21..57
FT   DISULFID        22..49
FT   DISULFID        30..47
FT   DISULFID        34..43
SQ   SEQUENCE   127 AA;  13597 MW;  70C1BD787A9D1C46 CRC64;
     MVCLLILGLV LEQVQVEGKS CCRSTLGRNC YNLCRVRGAQ KLCAGVCRCK LTSSGKCPTG
     FPKLALVSNS DEPDTVKYCN LGCRASMCDY MVNAAADDEE MKLYLENCGD ACVNFCNGDA
     GLTSLTA