ID   THND_HELPU              Reviewed;          46 AA.
AC   P60057;
DT   21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   21-NOV-2003, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Hellethionin-D;
OS   Helleborus purpurascens (Purple hellebore).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Ranunculales; Ranunculaceae; Ranunculoideae;
OC   Helleboreae; Helleborus.
OX   NCBI_TaxID=171899;
RN   [1]
RP   STRUCTURE BY NMR.
RX   PubMed=12600207; DOI=10.1021/bi020628h;
RA   Milbradt A.G., Kerek F., Moroder L., Renner C.;
RT   "Structural characterization of hellethionins from Helleborus
RT   purpurascens.";
RL   Biochemistry 42:2404-2411(2003).
CC   -!- FUNCTION: Thionins are small plant proteins which are toxic to animal
CC       cells. They seem to exert their toxic effect at the level of the cell
CC       membrane. Their precise function is not known.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the plant thionin (TC 1.C.44) family.
CC       {ECO:0000305}.
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DR   PDB; 1NBL; NMR; -; A=1-46.
DR   PDB; 3SZS; X-ray; 1.95 A; A/B/C/D/E/F/G=1-46.
DR   PDBsum; 1NBL; -.
DR   PDBsum; 3SZS; -.
DR   AlphaFoldDB; P60057; -.
DR   BMRB; P60057; -.
DR   SMR; P60057; -.
DR   EvolutionaryTrace; P60057; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1350.10; -; 1.
DR   InterPro; IPR001010; Thionin.
DR   InterPro; IPR036391; Thionin-like_sf.
DR   Pfam; PF00321; Thionin; 1.
DR   PRINTS; PR00287; THIONIN.
DR   SUPFAM; SSF57429; SSF57429; 1.
DR   PROSITE; PS00271; THIONIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Disulfide bond; Plant defense; Secreted; Toxin.
FT   CHAIN           1..46
FT                   /note="Hellethionin-D"
FT                   /id="PRO_0000221480"
FT   DISULFID        3..40
FT   DISULFID        4..32
FT   DISULFID        12..30
FT   DISULFID        16..26
FT   STRAND          2..6
FT                   /evidence="ECO:0007829|PDB:3SZS"
FT   HELIX           7..18
FT                   /evidence="ECO:0007829|PDB:3SZS"
FT   HELIX           23..29
FT                   /evidence="ECO:0007829|PDB:3SZS"
FT   STRAND          32..39
FT                   /evidence="ECO:0007829|PDB:3SZS"
SQ   SEQUENCE   46 AA;  4910 MW;  A5778F02EC9D3AA7 CRC64;
     KSCCRNTLAR NCYNACRFTG GSQPTCGILC DCIHVTTTTC PSSHPS