THND_TRIHA
ID THND_TRIHA Reviewed; 405 AA.
AC A0A0F9XIF1;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2015, sequence version 1.
DT 03-AUG-2022, entry version 19.
DE RecName: Full=FAD-dependent monooxygenase thnD {ECO:0000303|PubMed:33570538};
DE EC=1.-.-.- {ECO:0000305|PubMed:33570538};
DE AltName: Full=Trihazone biosynthesis cluster protein D {ECO:0000303|PubMed:33570538};
GN Name=thnD {ECO:0000303|PubMed:33570538}; ORFNames=THAR02_03176;
OS Trichoderma harzianum (Hypocrea lixii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.
OX NCBI_TaxID=5544;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T6776;
RX PubMed=26067977; DOI=10.1128/genomea.00647-15;
RA Baroncelli R., Piaggeschi G., Fiorini L., Bertolini E., Zapparata A.,
RA Pe M.E., Sarrocco S., Vannacci G.;
RT "Draft whole-genome sequence of the biocontrol agent Trichoderma harzianum
RT T6776.";
RL Genome Announc. 3:E0064715-E0064715(2015).
RN [2]
RP FUNCTION.
RX PubMed=33570538; DOI=10.1039/d0ob02545c;
RA Zhu Y., Wang J., Mou P., Yan Y., Chen M., Tang Y.;
RT "Genome mining of cryptic tetronate natural products from a PKS-NRPS
RT encoding gene cluster in Trichoderma harzianum t-22.";
RL Org. Biomol. Chem. 19:1985-1990(2021).
CC -!- FUNCTION: FAD-dependent monooxygenase; part of the gene cluster that
CC produces the tetronate natural products trihazones (PubMed:33570538).
CC Transcription analysis of thnD confirmed this gene is expressed, hence
CC its role in the biosynthetic pathway remains cryptic (PubMed:33570538).
CC The pathway begins with the formation of trihazone A by the hybrid PKS-
CC NRPS synthetase thnA and the trans-enoyl reductase thnE. Trihazone A is
CC further decarboxylated by the 2-oxoglutarate-dependent dioxygenase thnC
CC to produce trihazone D. The function of the FAD-dependent monooxygenase
CC thnD has still to be identified (PubMed:33570538).
CC {ECO:0000269|PubMed:33570538}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
CC -!- SIMILARITY: Belongs to the paxM FAD-dependent monooxygenase family.
CC {ECO:0000305}.
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DR EMBL; JOKZ01000069; KKP04696.1; -; Genomic_DNA.
DR EnsemblFungi; KKP04696; KKP04696; THAR02_03176.
DR OMA; FQGWAIA; -.
DR OrthoDB; 1248412at2759; -.
DR Proteomes; UP000034112; Unassembled WGS sequence.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR002938; FAD-bd.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR Pfam; PF01494; FAD_binding_3; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 3: Inferred from homology;
KW FAD; Flavoprotein; Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..405
FT /note="FAD-dependent monooxygenase thnD"
FT /id="PRO_0000455679"
FT BINDING 31..32
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:A6T923"
FT BINDING 308
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:A6T923"
FT BINDING 318..322
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:A6T923"
SQ SEQUENCE 405 AA; 45550 MW; 69F4BF2BE22C234E CRC64;
MHVLIAGAGL GGLTLAQNLR KRGISYEIFE RDEHKDARFQ GWAIALHTIT DELVASMPSD
MPDLRQATDH MSPLKIPTQI CLYPAESNVR VGWEDCPETP FIRAERYRLR NHLATNIPIQ
WGKRVQRIEH DDQGVAVYFE DGTGAKGDIL VGADGVKSVV RQQLLQKSHD EVLKIIPLAA
IVGELKLSGE AFKRQLQLSH GSYSLIDSEK GYLTFCSLHD VAPDGKSARY YWIMSRSDPT
IADPDHWLHK ATQQEKLDHA REVMSNREPK FREIFDLTPV EGVKKETHIW RDLELSSLPA
GRVVLMGDAA HVMTPFRGEG GYNTFIDAMA LAKILDRLDK EDKTHDIESI KNSVNEYNAE
MLERGVKSVQ LSREWVDGSK VNSKKPLLAP MKVIPFSEVP LEVAA
