ID   THNS1_HUMAN             Reviewed;         743 AA.
AC   Q8IYQ7; B3KWL1; D3DRV3; Q5VV21;
DT   12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   12-APR-2005, sequence version 2.
DT   03-AUG-2022, entry version 148.
DE   RecName: Full=Threonine synthase-like 1;
DE            Short=TSH1;
GN   Name=THNSL1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15164054; DOI=10.1038/nature02462;
RA   Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA   Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA   Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA   Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA   Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA   Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA   Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA   Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA   Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA   Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA   Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA   Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA   Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA   Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA   McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA   Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA   Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA   Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA   Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA   Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA   Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA   Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA   Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT   "The DNA sequence and comparative analysis of human chromosome 10.";
RL   Nature 429:375-381(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-281, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19608861; DOI=10.1126/science.1175371;
RA   Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C.,
RA   Olsen J.V., Mann M.;
RT   "Lysine acetylation targets protein complexes and co-regulates major
RT   cellular functions.";
RL   Science 325:834-840(2009).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the threonine synthase family. {ECO:0000305}.
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DR   EMBL; AK125249; BAG54173.1; -; mRNA.
DR   EMBL; AL512598; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471072; EAW86113.1; -; Genomic_DNA.
DR   EMBL; CH471072; EAW86114.1; -; Genomic_DNA.
DR   EMBL; BC035132; AAH35132.1; -; mRNA.
DR   CCDS; CCDS7147.1; -.
DR   RefSeq; NP_079114.3; NM_024838.4.
DR   RefSeq; XP_005252654.1; XM_005252597.2.
DR   RefSeq; XP_016872154.1; XM_017016665.1.
DR   AlphaFoldDB; Q8IYQ7; -.
DR   SMR; Q8IYQ7; -.
DR   BioGRID; 122980; 70.
DR   IntAct; Q8IYQ7; 23.
DR   STRING; 9606.ENSP00000434887; -.
DR   iPTMnet; Q8IYQ7; -.
DR   PhosphoSitePlus; Q8IYQ7; -.
DR   BioMuta; THNSL1; -.
DR   DMDM; 62511212; -.
DR   EPD; Q8IYQ7; -.
DR   jPOST; Q8IYQ7; -.
DR   MassIVE; Q8IYQ7; -.
DR   MaxQB; Q8IYQ7; -.
DR   PaxDb; Q8IYQ7; -.
DR   PeptideAtlas; Q8IYQ7; -.
DR   PRIDE; Q8IYQ7; -.
DR   ProteomicsDB; 71213; -.
DR   Antibodypedia; 25872; 93 antibodies from 20 providers.
DR   DNASU; 79896; -.
DR   Ensembl; ENST00000376356.5; ENSP00000365534.4; ENSG00000185875.13.
DR   Ensembl; ENST00000524413.5; ENSP00000434887.1; ENSG00000185875.13.
DR   GeneID; 79896; -.
DR   KEGG; hsa:79896; -.
DR   MANE-Select; ENST00000376356.5; ENSP00000365534.4; NM_024838.5; NP_079114.3.
DR   UCSC; uc001isi.6; human.
DR   CTD; 79896; -.
DR   GeneCards; THNSL1; -.
DR   HGNC; HGNC:26160; THNSL1.
DR   HPA; ENSG00000185875; Tissue enhanced (liver).
DR   MIM; 611260; gene.
DR   neXtProt; NX_Q8IYQ7; -.
DR   OpenTargets; ENSG00000185875; -.
DR   PharmGKB; PA134906927; -.
DR   VEuPathDB; HostDB:ENSG00000185875; -.
DR   eggNOG; KOG2616; Eukaryota.
DR   GeneTree; ENSGT00940000161144; -.
DR   HOGENOM; CLU_015170_3_2_1; -.
DR   InParanoid; Q8IYQ7; -.
DR   OMA; DCLAAIH; -.
DR   OrthoDB; 1361511at2759; -.
DR   PhylomeDB; Q8IYQ7; -.
DR   TreeFam; TF329641; -.
DR   PathwayCommons; Q8IYQ7; -.
DR   SignaLink; Q8IYQ7; -.
DR   BioGRID-ORCS; 79896; 4 hits in 1076 CRISPR screens.
DR   ChiTaRS; THNSL1; human.
DR   GenomeRNAi; 79896; -.
DR   Pharos; Q8IYQ7; Tdark.
DR   PRO; PR:Q8IYQ7; -.
DR   Proteomes; UP000005640; Chromosome 10.
DR   RNAct; Q8IYQ7; protein.
DR   Bgee; ENSG00000185875; Expressed in secondary oocyte and 144 other tissues.
DR   Genevisible; Q8IYQ7; HS.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   CDD; cd00464; SK; 1.
DR   Gene3D; 3.40.50.1100; -; 2.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.90.1380.10; -; 1.
DR   HAMAP; MF_00109; Shikimate_kinase; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR031322; Shikimate/glucono_kinase.
DR   InterPro; IPR000623; Shikimate_kinase/TSH1.
DR   InterPro; IPR029144; Thr_synth_N.
DR   InterPro; IPR037158; Thr_synth_N_sf.
DR   InterPro; IPR004450; Thr_synthase-like.
DR   InterPro; IPR036052; Trypto_synt_PLP_dependent.
DR   Pfam; PF01202; SKI; 1.
DR   Pfam; PF14821; Thr_synth_N; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF53686; SSF53686; 1.
DR   TIGRFAMs; TIGR00260; thrC; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Pyridoxal phosphate; Reference proteome.
FT   CHAIN           1..743
FT                   /note="Threonine synthase-like 1"
FT                   /id="PRO_0000185646"
FT   MOD_RES         281
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0007744|PubMed:19608861"
FT   MOD_RES         351
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
FT   VARIANT         154
FT                   /note="L -> P (in dbSNP:rs35827877)"
FT                   /id="VAR_052545"
FT   VARIANT         239
FT                   /note="P -> R (in dbSNP:rs41279890)"
FT                   /id="VAR_061893"
FT   VARIANT         248
FT                   /note="A -> E (in dbSNP:rs34929144)"
FT                   /id="VAR_052546"
FT   VARIANT         399
FT                   /note="Q -> R (in dbSNP:rs41279894)"
FT                   /id="VAR_058869"
FT   CONFLICT        94
FT                   /note="T -> N (in Ref. 4; AAH35132)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        705
FT                   /note="H -> R (in Ref. 4; AAH35132)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   743 AA;  83070 MW;  0C166A57EF34A682 CRC64;
     MLHFNRCHHL KKITQKCFSS IHVKTDKHAQ RFLSRTFALA ELRKSWYSTH SLVGDKNIIL
     MGPPGAGKTT VGRIIGQKLG CCVIDVDDDI LEKTWNMSVS EKLQDVGNEQ FLEEEGKAVL
     NFSASGSVIS LTGSNPMHDA SMWHLKKNGI IVYLDVPLLD LICRLKLMKT DRIVGQNSGT
     SMKDLLKFRR QYYKKWYDAR VFCESGASPE EVADKVLNAI KRYQDVDSET FISTRHVWPE
     DCEQKVSAKF FSEAVIEGLA SDGGLFVPAK EFPKLSCGEW KSLVGATYVE RAQILLERCI
     HPADIPAARL GEMIETAYGE NFACSKIAPV RHLSGNQFIL ELFHGPTGSF KDLSLQLMPH
     IFAHCIPPSC NYMILVATSG DTGSAVLNGF SRLNKNDKQR IAVVAFFPEN GVSDFQKAQI
     IGSQRENGWA VGVESDFDFC QTAIKRIFND SDFTGFLTVE YGTILSSANS INWGRLLPQV
     VYHASAYLDL VSQGFISFGS PVDVCIPTGN FGNILAAVYA KMMGIPIRKF ICASNQNHVL
     TDFIKTGHYD LRERKLAQTF SPSIDILKSS NLERHLHLMA NKDGQLMTEL FNRLESQHHF
     QIEKALVEKL QQDFVADWCS EGECLAAINS TYNTSGYILD PHTAVAKVVA DRVQDKTCPV
     IISSTAHYSK FAPAIMQALK IKEINETSSS QLYLLGSYNA LPPLHEALLE RTKQQEKMEY
     QVCAADMNVL KSHVEQLVQN QFI