ID   THNW1_NIGSA             Reviewed;          35 AA.
AC   C0HJH9;
DT   07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2017, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=Thionin NsW1 {ECO:0000303|Ref.1};
DE   Flags: Fragment;
OS   Nigella sativa (Black cumin).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Ranunculales; Ranunculaceae; Ranunculoideae;
OC   Nigelleae; Nigella.
OX   NCBI_TaxID=555479 {ECO:0000303|Ref.1};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, PRESENCE OF DISULFIDE BONDS, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Seed {ECO:0000303|Ref.1};
RX   DOI=10.1007/s10989-016-9549-1;
RA   Vasilchenko A.S., Smirnov A.N., Zavriev S.K., Grishin E.V.,
RA   Vasilchenko A.V., Rogozhin E.A.;
RT   "Novel Thionins from Black Seed (Nigella sativa L.) Demonstrate
RT   Antimicrobial Activity.";
RL   Int. J. Pept. Res. Ther. 22:1-10(2016).
CC   -!- FUNCTION: Antimicrobial peptide disrupting membranes. Has antibacterial
CC       against Gram-positive bacteria S.aureus (MIC=6.5 uM) and B.subtilis
CC       (MIC=3.25 uM) but not against Gram-negative bacterium E.coli. Has
CC       antifungal activity against C.albicans (MIC=1.63 uM).
CC       {ECO:0000269|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- PTM: Contains 4 disulfide bonds. {ECO:0000269|Ref.1}.
CC   -!- MASS SPECTROMETRY: Mass=5067; Method=MALDI;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- SIMILARITY: Belongs to the plant thionin (TC 1.C.44) family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; C0HJH9; -.
DR   SMR; C0HJH9; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IDA:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1350.10; -; 1.
DR   InterPro; IPR001010; Thionin.
DR   InterPro; IPR036391; Thionin-like_sf.
DR   Pfam; PF00321; Thionin; 1.
DR   PRINTS; PR00287; THIONIN.
DR   SUPFAM; SSF57429; SSF57429; 1.
DR   PROSITE; PS00271; THIONIN; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing;
KW   Disulfide bond; Fungicide; Plant defense; Secreted; Toxin.
FT   PEPTIDE         1..>35
FT                   /note="Thionin NsW1"
FT                   /evidence="ECO:0000269|Ref.1"
FT                   /id="PRO_0000440579"
FT   DISULFID        4..32
FT                   /evidence="ECO:0000250|UniProtKB:P21742"
FT   DISULFID        12..30
FT                   /evidence="ECO:0000250|UniProtKB:P21742"
FT   DISULFID        16..26
FT                   /evidence="ECO:0000250|UniProtKB:P21742"
FT   DISULFID        30..?
FT                   /evidence="ECO:0000250|UniProtKB:P21742"
FT   NON_TER         35
FT                   /evidence="ECO:0000303|Ref.1"
SQ   SEQUENCE   35 AA;  3934 MW;  4EB4B73E41983835 CRC64;
     KSCCKNTLGR NCYNTCRFMK KPRKTCSGLC GCKIS