ID   THN_BRARP               Reviewed;         133 AA.
AC   Q9SBK8;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Thionin;
DE   Contains:
DE     RecName: Full=Thionin;
DE   Contains:
DE     RecName: Full=Acidic protein;
DE   Flags: Precursor;
GN   Name=THI2;
OS   Brassica rapa subsp. pekinensis (Chinese cabbage) (Brassica pekinensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=51351;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Flower bud;
RA   Lee S.Y., Cho M.J., Choi Y.O.;
RT   "Molecular cloning and characterization of flower specific thionin in
RT   Chinese cabbage.";
RL   Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Thionins are small plant proteins which are toxic to animal
CC       cells. They seem to exert their toxic effect at the level of the cell
CC       membrane. Their precise function is not known.
CC   -!- TISSUE SPECIFICITY: Expressed in flowers.
CC   -!- SIMILARITY: Belongs to the plant thionin (TC 1.C.44.1) family.
CC       {ECO:0000305}.
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DR   EMBL; AF090836; AAF21800.1; -; mRNA.
DR   AlphaFoldDB; Q9SBK8; -.
DR   SMR; Q9SBK8; -.
DR   eggNOG; ENOG502SUEZ; Eukaryota.
DR   InParanoid; Q9SBK8; -.
DR   Proteomes; UP000011750; Unplaced.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1350.10; -; 1.
DR   InterPro; IPR001010; Thionin.
DR   InterPro; IPR036391; Thionin-like_sf.
DR   PANTHER; PTHR33920; PTHR33920; 1.
DR   Pfam; PF00321; Thionin; 1.
DR   PRINTS; PR00287; THIONIN.
DR   SUPFAM; SSF57429; SSF57429; 1.
DR   PROSITE; PS00271; THIONIN; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Plant defense; Reference proteome; Signal; Toxin.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..70
FT                   /note="Thionin"
FT                   /id="PRO_0000034147"
FT   CHAIN           71..133
FT                   /note="Acidic protein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000034148"
FT   DISULFID        27..64
FT                   /evidence="ECO:0000250"
FT   DISULFID        28..56
FT                   /evidence="ECO:0000250"
FT   DISULFID        40..50
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   133 AA;  13961 MW;  4BBC3A8A8B5CD964 CRC64;
     MEGKTVILGV IIMSLVMAQN QVEAKICCPR TIDRNIYNAC RLTGASMTNC ANLSGCKIVS
     GTTCPPGYTH DILQNYGDAV NEYCKLGCAS SVCGALTTLK NSMQVNCEGA VSQCTNACSN
     FCTKGSAKVV ETA