ID   THO5B_ARATH             Reviewed;         819 AA.
AC   F4K4J0; Q94BS3; Q9FMM6;
DT   19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=THO complex subunit 5B;
DE   AltName: Full=THO complex subunit 5;
DE            Short=AtTHO5;
GN   Name=THO5B; Synonyms=THO5, THOC5A; OrderedLocusNames=At5g42920;
GN   ORFNames=MBD2.12;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA   Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT   features of the regions of 1,191,918 bp covered by seventeen physically
RT   assigned P1 clones.";
RL   DNA Res. 4:401-414(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBUNIT.
RX   PubMed=20634427; DOI=10.1073/pnas.0911341107;
RA   Yelina N.E., Smith L.M., Jones A.M., Patel K., Kelly K.A., Baulcombe D.C.;
RT   "Putative Arabidopsis THO/TREX mRNA export complex is involved in transgene
RT   and endogenous siRNA biosynthesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:13948-13953(2010).
CC   -!- FUNCTION: Acts as component of the THO subcomplex of the TREX complex
CC       which is thought to couple mRNA transcription, processing and nuclear
CC       export. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the THO complex, which is composed of THO1, THO2,
CC       THO3, THO5, THO6 and THO7. {ECO:0000269|PubMed:20634427}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=F4K4J0-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=F4K4J0-2; Sequence=VSP_053743;
CC   -!- SIMILARITY: Belongs to the THOC5 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB09194.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB008264; BAB09194.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED94887.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED94888.1; -; Genomic_DNA.
DR   EMBL; AY039925; AAK64029.1; -; mRNA.
DR   EMBL; AY091346; AAM14285.1; -; mRNA.
DR   RefSeq; NP_568616.1; NM_123657.3. [F4K4J0-2]
DR   RefSeq; NP_974873.1; NM_203144.4. [F4K4J0-1]
DR   AlphaFoldDB; F4K4J0; -.
DR   SMR; F4K4J0; -.
DR   BioGRID; 19554; 45.
DR   IntAct; F4K4J0; 2.
DR   STRING; 3702.AT5G42920.2; -.
DR   iPTMnet; F4K4J0; -.
DR   PaxDb; F4K4J0; -.
DR   PRIDE; F4K4J0; -.
DR   ProteomicsDB; 246460; -. [F4K4J0-1]
DR   EnsemblPlants; AT5G42920.1; AT5G42920.1; AT5G42920. [F4K4J0-2]
DR   EnsemblPlants; AT5G42920.2; AT5G42920.2; AT5G42920. [F4K4J0-1]
DR   GeneID; 834304; -.
DR   Gramene; AT5G42920.1; AT5G42920.1; AT5G42920. [F4K4J0-2]
DR   Gramene; AT5G42920.2; AT5G42920.2; AT5G42920. [F4K4J0-1]
DR   KEGG; ath:AT5G42920; -.
DR   Araport; AT5G42920; -.
DR   TAIR; locus:2160006; AT5G42920.
DR   eggNOG; KOG2216; Eukaryota.
DR   HOGENOM; CLU_019074_0_0_1; -.
DR   InParanoid; F4K4J0; -.
DR   OMA; SLMKLKW; -.
DR   OrthoDB; 1048314at2759; -.
DR   PRO; PR:F4K4J0; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; F4K4J0; baseline and differential.
DR   Genevisible; F4K4J0; AT.
DR   GO; GO:0000347; C:THO complex; IDA:UniProtKB.
DR   GO; GO:0000445; C:THO complex part of transcription export complex; IBA:GO_Central.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0006406; P:mRNA export from nucleus; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0032786; P:positive regulation of DNA-templated transcription, elongation; IBA:GO_Central.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   InterPro; IPR019163; THO_Thoc5.
DR   PANTHER; PTHR13375; PTHR13375; 1.
DR   Pfam; PF09766; FmiP_Thoc5; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; mRNA processing; mRNA splicing; mRNA transport;
KW   Nucleus; Reference proteome; RNA-binding; Transport.
FT   CHAIN           1..819
FT                   /note="THO complex subunit 5B"
FT                   /id="PRO_0000425590"
FT   REGION          285..332
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        285..302
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        312..330
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..117
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14593172"
FT                   /id="VSP_053743"
SQ   SEQUENCE   819 AA;  92577 MW;  8F4D02F6E600BC0F CRC64;
     MEDGEIEEGM VTADEFPTPE VTTIETIQPP REPGKSPLEL LRESKTSVEE IVAKMLSMKK
     QGNHKSEIRE LLTQMFLNFV NLRQANRAIL TEEDKVKAET ERAKAPVDFT TLQLHNLMYE
     KSHYVKAIKA CRDFKSKYPD IDLVPEQDFF RHAPEAIKDQ SLSSDSSHVL MPKRLNFELH
     QRKELCKHRA RLEQQKKSLL ETIAERKKFL SSLPLHLKSL KKASLPVQNH LGIQHTKKLK
     QHNLAELLPP PLYVLYSQLL AQKEAFEESI ELEVVGSLKD AQAYARQQSR KDSGMSSNTE
     SSRLEDDGPD DDDDGQRRRK RPKKLTSKEG SDKAGLYQVH PLKIVLHIYD DEIPDTKSLK
     LVILKFEYLL KLNVVCVGAE GSQDGPEKNI FCNLFPDDAG LEPPHQSTKL ILGDGQTFDE
     NRTSRPYKWV QHLAGIDISP VLLGQEAHNT DPAKSDTFVP DLSLYRQQHR VQTVLRRIRL
     RKKAHLALAE QLDLLMKHEL PVVNCEDAPW ALHKVLCALD SWLHIQSSAS KSCSLTLNSV
     EQVPEPMEID VDGRSISGKE DFESIREDGE LPSLVTAAAS LTSSNHTPSK VSNQARSRQL
     ALMTKNLDSP ISKGKSPSFK KYEDDLDLVL DDDSEIDEPT GRTEAHVEEL CPEKADNSWV
     DYGSREFALV FSRKTDGGKL WKLEAMVQIS MEYPLRPPLF SLSLHASSSS GNENGTNESD
     HYNELRAMEA EVNLHMLKII PSDQENYLLS HQIRCLAMLF DYYVDDPSPD SKRGTATTVV
     DVGLCKPVDG KLLVRSFRGR DHRKMISWKG RGCASGYPC