THOC3_ARATH
ID THOC3_ARATH Reviewed; 315 AA.
AC Q9FKT5;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=THO complex subunit 3;
DE AltName: Full=TEX1 homolog;
DE Short=AtTEX1;
GN Name=THO3; Synonyms=TEX1; OrderedLocusNames=At5g56130; ORFNames=MDA7.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20798330; DOI=10.1105/tpc.110.076638;
RA Jauvion V., Elmayan T., Vaucheret H.;
RT "The conserved RNA trafficking proteins HPR1 and TEX1 are involved in the
RT production of endogenous and exogenous small interfering RNA in
RT Arabidopsis.";
RL Plant Cell 22:2697-2709(2010).
RN [5]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND MUTAGENESIS OF
RP SER-86.
RX PubMed=20634427; DOI=10.1073/pnas.0911341107;
RA Yelina N.E., Smith L.M., Jones A.M., Patel K., Kelly K.A., Baulcombe D.C.;
RT "Putative Arabidopsis THO/TREX mRNA export complex is involved in transgene
RT and endogenous siRNA biosynthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:13948-13953(2010).
CC -!- FUNCTION: Acts as component of the THO subcomplex of the TREX complex
CC which is thought to couple mRNA transcription, processing and nuclear
CC export. Contributes to the integrity of the endogenous trans-acting
CC small interfering RNA (ta-siRNA) pathway. May process or transport a
CC long RNA molecule so that it can be a template for secondary siRNA
CC production. May participate in the trafficking of siRNA precursors to
CC the ARGONAUTE catalytic center. Required for the generation of
CC functional messenger ribonucleoproteins (mRNPs).
CC {ECO:0000269|PubMed:20634427, ECO:0000269|PubMed:20798330}.
CC -!- SUBUNIT: Component of the THO complex, which is composed of THO1, THO2,
CC THO3, THO5, THO6 and THO7. {ECO:0000269|PubMed:20634427}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Developmental defects as well as reduced levels
CC of endogenous trans-acting small interfering RNA (ta-siRNA).
CC {ECO:0000269|PubMed:20798330}.
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DR EMBL; AB011476; BAB09295.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96724.1; -; Genomic_DNA.
DR EMBL; AY093120; AAM13119.1; -; mRNA.
DR EMBL; BT000174; AAN15493.1; -; mRNA.
DR RefSeq; NP_200424.1; NM_124995.5.
DR AlphaFoldDB; Q9FKT5; -.
DR SMR; Q9FKT5; -.
DR BioGRID; 20956; 61.
DR STRING; 3702.AT5G56130.1; -.
DR PaxDb; Q9FKT5; -.
DR PRIDE; Q9FKT5; -.
DR ProteomicsDB; 246464; -.
DR EnsemblPlants; AT5G56130.1; AT5G56130.1; AT5G56130.
DR GeneID; 835712; -.
DR Gramene; AT5G56130.1; AT5G56130.1; AT5G56130.
DR KEGG; ath:AT5G56130; -.
DR Araport; AT5G56130; -.
DR TAIR; locus:2161840; AT5G56130.
DR eggNOG; KOG1407; Eukaryota.
DR HOGENOM; CLU_045202_0_0_1; -.
DR InParanoid; Q9FKT5; -.
DR OMA; YWLAYST; -.
DR OrthoDB; 1014314at2759; -.
DR PhylomeDB; Q9FKT5; -.
DR PRO; PR:Q9FKT5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FKT5; baseline and differential.
DR Genevisible; Q9FKT5; AT.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:TAIR.
DR GO; GO:0000347; C:THO complex; IDA:UniProtKB.
DR GO; GO:0000445; C:THO complex part of transcription export complex; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0031047; P:gene silencing by RNA; IMP:TAIR.
DR GO; GO:0006406; P:mRNA export from nucleus; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR GO; GO:0010267; P:ta-siRNA processing; IMP:TAIR.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR040132; Tex1/THOC3.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR22839; PTHR22839; 1.
DR Pfam; PF00400; WD40; 3.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; mRNA transport; Nucleus;
KW Reference proteome; Repeat; RNA-binding; RNA-mediated gene silencing;
KW Transport; WD repeat.
FT CHAIN 1..315
FT /note="THO complex subunit 3"
FT /id="PRO_0000425584"
FT REPEAT 18..57
FT /note="WD 1"
FT REPEAT 64..104
FT /note="WD 2"
FT REPEAT 106..145
FT /note="WD 3"
FT REPEAT 189..228
FT /note="WD 4"
FT REPEAT 231..270
FT /note="WD 5"
FT REPEAT 272..311
FT /note="WD 6"
FT MUTAGEN 86
FT /note="S->F: In attex1-2; reduced levels of endogenous
FT trans-acting small interfering RNA (ta-siRNA)."
FT /evidence="ECO:0000269|PubMed:20634427"
SQ SEQUENCE 315 AA; 35375 MW; 9935657E83ACD62B CRC64;
MEETTIPFKS LHSREYQGHK KKVHSVAWNS NGTKLASGSV DQTARIWNIE PHGHSKAKDL
ELKGHTDSVD QLCWDPKHSD LVATASGDKS VRLWDARSGK CTQQVELSGE NINITYKPDG
THVAVGNRDD ELTILDVRKF KPLHRRKFNY EVNEIAWNMP GDFFFLTTGL GTVEVLSYPS
LKPLDTLTAH TAGCYCIAID PKGRYFAVGS ADSLVSLWDI SDMLCLRTFT KLEWPVRTIS
FNYSGEYIAS ASEDLFIDIA NVQTGRTVHQ IPCRAAMNSV EWNPKYNLLA YAGDDKNPKY
NTDEGVFRIF GFESS
