THOC4_XENTR
ID THOC4_XENTR Reviewed; 260 AA.
AC Q28FB9;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=THO complex subunit 4;
DE Short=Tho4;
DE AltName: Full=Aly/REF export factor;
GN Name=alyref; Synonyms=thoc4; ORFNames=TEgg022b23.1;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Egg;
RG Sanger Xenopus tropicalis EST/cDNA project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Export adapter involved in nuclear export of spliced and
CC unspliced mRNA. Binds mRNA which is thought to be transferred to the
CC NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the
CC TREX complex which is thought to couple mRNA transcription, processing
CC and nuclear export, and specifically associates with spliced mRNA and
CC not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a
CC transcription-independent mechanism, binds to mRNA upstream of the
CC exon-junction complex (EJC) and is recruited in a splicing- and cap-
CC dependent manner to a region near the 5' end of the mRNA where it
CC functions in mRNA export to the cytoplasm. Involved in the nuclear
CC export of intronless mRNA. Involved in transcription elongation and
CC genome stability (By similarity). {ECO:0000250|UniProtKB:Q86V81}.
CC -!- FUNCTION: Acts as chaperone and promotes the dimerization of
CC transcription factors containing basic leucine zipper (bZIP) domains
CC and thereby promotes transcriptional activation.
CC {ECO:0000250|UniProtKB:Q86V81}.
CC -!- SUBUNIT: Component of the transcription/export (TREX) complex; TREX
CC seems to have a dynamic structure involving ATP-dependent remodeling
CC (By similarity). {ECO:0000250|UniProtKB:Q86V81}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q86V81}. Nucleus
CC speckle {ECO:0000250|UniProtKB:Q86V81}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q86V81}. Note=Travels to the cytoplasm as part
CC of the exon junction complex (EJC) bound to mRNA.
CC {ECO:0000250|UniProtKB:Q86V81}.
CC -!- SIMILARITY: Belongs to the ALYREF family. {ECO:0000305}.
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DR EMBL; CR762045; CAJ81934.1; -; mRNA.
DR EMBL; BC135842; AAI35843.1; -; mRNA.
DR RefSeq; NP_001017027.1; NM_001017027.2.
DR AlphaFoldDB; Q28FB9; -.
DR BMRB; Q28FB9; -.
DR SMR; Q28FB9; -.
DR PaxDb; Q28FB9; -.
DR DNASU; 549781; -.
DR Ensembl; ENSXETT00000011893; ENSXETP00000011893; ENSXETG00000005391.
DR GeneID; 549781; -.
DR KEGG; xtr:549781; -.
DR CTD; 10189; -.
DR Xenbase; XB-GENE-973553; alyref.
DR eggNOG; KOG0533; Eukaryota.
DR InParanoid; Q28FB9; -.
DR OMA; DMYDGPK; -.
DR OrthoDB; 1369069at2759; -.
DR PhylomeDB; Q28FB9; -.
DR TreeFam; TF313312; -.
DR Reactome; R-XTR-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-XTR-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-XTR-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-XTR-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-XTR-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-XTR-72187; mRNA 3'-end processing.
DR Proteomes; UP000008143; Chromosome 10.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000005391; Expressed in gastrula and 32 other tissues.
DR ExpressionAtlas; Q28FB9; baseline.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0062153; F:C5-methylcytidine-containing RNA binding; ISS:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0006406; P:mRNA export from nucleus; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0006405; P:RNA export from nucleus; ISS:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR025715; FoP_C.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF13865; FoP_duplication; 1.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM01218; FoP_duplication; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW Chaperone; Cytoplasm; mRNA processing; mRNA splicing; mRNA transport;
KW Nucleus; Reference proteome; RNA-binding; Transport.
FT CHAIN 1..260
FT /note="THO complex subunit 4"
FT /id="PRO_0000378582"
FT DOMAIN 109..186
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 189..260
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 189..203
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 260 AA; 27156 MW; BA2F1D6B247B27B4 CRC64;
MGDKMDMSLD DIIKLNRSQR PAGRGRGGGR GARGGSARGG AGGRIGGGRG GGGGGAAGGG
GPMRSRPVLT RGGRNRPAPY SRPKQLPDKW QHDLFDSGFG TGAGMETGGK LLVSNLDFGV
SDADIQELFA EFGTLKKAAV HYDRSGRSLG TADVHFERKA DALKAMKQYN GVPLDGRPMN
IQLVTSQIEA QRRPIQSQSR GGGITRPRGG AIGFSGANRR GGRGGNRGRG RGAGRNPKQQ
LSAEELDAQL DAYNARMDTS
