THOC5_DROME
ID THOC5_DROME Reviewed; 616 AA.
AC Q9W1F4; Q95RL0;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=THO complex subunit 5;
GN Name=thoc5; ORFNames=CG2980;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND IDENTIFICATION IN THE THO
RP COMPLEX.
RX PubMed=15133499; DOI=10.1038/nsmb759;
RA Rehwinkel J., Herold A., Gari K., Koecher T., Rode M., Ciccarelli F.L.,
RA Wilm M., Izaurralde E.;
RT "Genome-wide analysis of mRNAs regulated by the THO complex in
RT Drosophila.";
RL Nat. Struct. Mol. Biol. 11:558-566(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-237, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [6]
RP INTERACTION WITH PIWI, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=28472469; DOI=10.1093/nar/gkx355;
RA Ilyin A.A., Ryazansky S.S., Doronin S.A., Olenkina O.M., Mikhaleva E.A.,
RA Yakushev E.Y., Abramov Y.A., Belyakin S.N., Ivankin A.V., Pindyurin A.V.,
RA Gvozdev V.A., Klenov M.S., Shevelyov Y.Y.;
RT "Piwi interacts with chromatin at nuclear pores and promiscuously binds
RT nuclear transcripts in Drosophila ovarian somatic cells.";
RL Nucleic Acids Res. 45:7666-7680(2017).
RN [7]
RP INTERACTION WITH CG9890.
RX PubMed=30713769;
RA Fursova N.A., Nikolenko J.V., Soshnikova N.V., Mazina M.Y., Vorobyova N.E.,
RA Krasnov A.N.;
RT "Zinc Finger Protein CG9890 - New Component of ENY2-Containing Complexes of
RT Drosophila.";
RL Acta Naturae 10:110-114(2018).
CC -!- FUNCTION: The THO complex is required for cell proliferation and for
CC proper export of heat-shock mRNAs under heat stress.
CC {ECO:0000269|PubMed:15133499}.
CC -!- SUBUNIT: Component of the THO complex, composed of at least e(y)2,
CC HPR1, THO2, THOC5, THOC6 and THOC7 (PubMed:15133499). Interacts with
CC piwi; the interaction might be partly RNA-mediated (PubMed:28472469).
CC Interacts with CG9890 (PubMed:30713769). {ECO:0000269|PubMed:15133499,
CC ECO:0000269|PubMed:28472469, ECO:0000269|PubMed:30713769}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC Nucleus membrane {ECO:0000269|PubMed:28472469}. Note=Shuttles between
CC nucleus and cytoplasm. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in ovaries (at protein level).
CC {ECO:0000269|PubMed:28472469}.
CC -!- SIMILARITY: Belongs to the THOC5 family. {ECO:0000305}.
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DR EMBL; AJ620304; CAF04333.1; -; mRNA.
DR EMBL; AE013599; AAF47114.2; -; Genomic_DNA.
DR EMBL; AY061305; AAL28853.1; -; mRNA.
DR RefSeq; NP_611856.1; NM_138012.3.
DR AlphaFoldDB; Q9W1F4; -.
DR SMR; Q9W1F4; -.
DR BioGRID; 63400; 22.
DR IntAct; Q9W1F4; 2.
DR STRING; 7227.FBpp0072056; -.
DR iPTMnet; Q9W1F4; -.
DR PaxDb; Q9W1F4; -.
DR PRIDE; Q9W1F4; -.
DR DNASU; 37810; -.
DR EnsemblMetazoa; FBtr0072147; FBpp0072056; FBgn0034939.
DR GeneID; 37810; -.
DR KEGG; dme:Dmel_CG2980; -.
DR UCSC; CG2980-RA; d. melanogaster.
DR CTD; 8563; -.
DR FlyBase; FBgn0034939; thoc5.
DR VEuPathDB; VectorBase:FBgn0034939; -.
DR eggNOG; KOG2216; Eukaryota.
DR GeneTree; ENSGT00390000013777; -.
DR HOGENOM; CLU_023759_0_0_1; -.
DR InParanoid; Q9W1F4; -.
DR OMA; EAYCDIV; -.
DR OrthoDB; 1048314at2759; -.
DR PhylomeDB; Q9W1F4; -.
DR Reactome; R-DME-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-DME-72187; mRNA 3'-end processing.
DR Reactome; R-DME-73856; RNA Polymerase II Transcription Termination.
DR SignaLink; Q9W1F4; -.
DR BioGRID-ORCS; 37810; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37810; -.
DR PRO; PR:Q9W1F4; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034939; Expressed in eye disc (Drosophila) and 25 other tissues.
DR Genevisible; Q9W1F4; DM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0032991; C:protein-containing complex; IPI:FlyBase.
DR GO; GO:0000347; C:THO complex; IDA:UniProtKB.
DR GO; GO:0000445; C:THO complex part of transcription export complex; IBA:GO_Central.
DR GO; GO:0000346; C:transcription export complex; ISS:FlyBase.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0043021; F:ribonucleoprotein complex binding; IDA:UniProtKB.
DR GO; GO:0007140; P:male meiotic nuclear division; IMP:FlyBase.
DR GO; GO:0006406; P:mRNA export from nucleus; IBA:GO_Central.
DR GO; GO:0031990; P:mRNA export from nucleus in response to heat stress; IC:FlyBase.
DR GO; GO:0032786; P:positive regulation of DNA-templated transcription, elongation; IBA:GO_Central.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR019163; THO_Thoc5.
DR PANTHER; PTHR13375; PTHR13375; 1.
DR Pfam; PF09766; FmiP_Thoc5; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Membrane; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..616
FT /note="THO complex subunit 5"
FT /id="PRO_0000310562"
FT MOD_RES 237
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 616 AA; 70889 MW; F3CAD9D2E83A8A1E CRC64;
METILNTQFD QLQKAIEGIK EAKSKGQSTD EARIATTLMF VALKHANREV KHKIKAGRDK
LHLEKCKVDL SRLQLQNLLY EVSHLKKEIV RCQKFKSRDT SLELLSEAEY SSELPDMPND
AEELSSHKQH LHRLDCELRL RKALDKQYSA LLSSKQELLQ DNLSHAQRYV SFAPALRTLQ
SATKPLHDAL QLSLDVEWKL SAVVKYLPKP LYVLFIVLQS LQRVSEEQSF TAEVVGYESE
AQMQELLKEK KCSTLNYRQT KKESGDREKI ADKNNLDDSL APHPLHICLT IGAAGSNQLV
LQIRYFDFIK CATVWGQLNC SRNANGQGDT NFVHHLLRHL YPNDLGNELP IPGIQYELRS
SDLTAEECVR YLKAKDYGKP FCWLQSMCSI ATVNSSMLYK HELNLNKNNK EIIDRIARRL
NSWQRLSQQI RGLTFKDIDL YSLQENIYPA GLSCSLVQWT AITHEELHSQ ISDALNSSSA
DKGITYNSYR AVIVRGSAKM ECFIRIPSNY PLEMPLWILN VHWNGCLTAQ NNSAIKMMEF
WTNSLQPKHL EPNDCILYAQ LFRTIYSFDI FLETEGSMQN TIEYNKEKPY ISAFAKRSRS
RPYKYIKKGS VYAFKQ
