ID   THOC6_DANRE             Reviewed;         323 AA.
AC   Q5XJS5;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=THO complex subunit 6 homolog;
DE   AltName: Full=WD repeat-containing protein 58;
GN   Name=thoc6; Synonyms=wdr58; ORFNames=zgc:101618;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=23621916; DOI=10.1186/1750-1172-8-62;
RA   Beaulieu C.L., Huang L., Innes A.M., Akimenko M.A., Puffenberger E.G.,
RA   Schwartz C., Jerry P., Ober C., Hegele R.A., McLeod D.R.,
RA   Schwartzentruber J., Majewski J., Bulman D.E., Parboosingh J.S.,
RA   Boycott K.M.;
RT   "Intellectual disability associated with a homozygous missense mutation in
RT   THOC6.";
RL   Orphanet J. Rare Dis. 8:62-62(2013).
CC   -!- FUNCTION: Acts as component of the THO subcomplex of the TREX complex
CC       which is thought to couple mRNA transcription, processing and nuclear
CC       export, and which specifically associates with spliced mRNA and not
CC       with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a
CC       transcription-independent mechanism, binds to mRNA upstream of the
CC       exon-junction complex (EJC) and is recruited in a splicing- and cap-
CC       dependent manner to a region near the 5' end of the mRNA where it
CC       functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the THO subcomplex of the transcription/export
CC       (TREX) complex which seems to have a dynamic structure involving ATP-
CC       dependent remodeling. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Nucleus speckle
CC       {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Highly expressed in the developing midbrain and
CC       the eyes at 24 hpf. The expression becomes restricted afterwards to the
CC       posterior part of the midbrain and the midbrain-hindbrain boundary.
CC       {ECO:0000269|PubMed:23621916}.
CC   -!- SIMILARITY: Belongs to the WD repeat THOC6 family. {ECO:0000305}.
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DR   EMBL; BC083222; AAH83222.1; -; mRNA.
DR   RefSeq; NP_001006095.1; NM_001006095.1.
DR   AlphaFoldDB; Q5XJS5; -.
DR   SMR; Q5XJS5; -.
DR   STRING; 7955.ENSDARP00000055337; -.
DR   PaxDb; Q5XJS5; -.
DR   GeneID; 100004014; -.
DR   KEGG; dre:100004014; -.
DR   CTD; 79228; -.
DR   ZFIN; ZDB-GENE-041010-198; thoc6.
DR   eggNOG; KOG0649; Eukaryota.
DR   InParanoid; Q5XJS5; -.
DR   OrthoDB; 760323at2759; -.
DR   PhylomeDB; Q5XJS5; -.
DR   Reactome; R-DRE-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR   Reactome; R-DRE-72187; mRNA 3'-end processing.
DR   Reactome; R-DRE-73856; RNA Polymerase II Transcription Termination.
DR   PRO; PR:Q5XJS5; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR   GO; GO:0000347; C:THO complex; IBA:GO_Central.
DR   GO; GO:0000346; C:transcription export complex; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0006406; P:mRNA export from nucleus; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR042626; THOC6.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR44411; PTHR44411; 1.
DR   Pfam; PF00400; WD40; 1.
DR   SMART; SM00320; WD40; 3.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; Developmental protein; mRNA processing; mRNA splicing;
KW   mRNA transport; Nucleus; Reference proteome; Repeat; RNA-binding;
KW   Transport; WD repeat.
FT   CHAIN           1..323
FT                   /note="THO complex subunit 6 homolog"
FT                   /id="PRO_0000233161"
FT   REPEAT          6..45
FT                   /note="WD 1"
FT   REPEAT          58..96
FT                   /note="WD 2"
FT   REPEAT          108..147
FT                   /note="WD 3"
FT   REPEAT          150..189
FT                   /note="WD 4"
FT   REPEAT          200..238
FT                   /note="WD 5"
FT   REPEAT          279..321
FT                   /note="WD 6"
SQ   SEQUENCE   323 AA;  35368 MW;  3C0279409EE11534 CRC64;
     MGPVELLHMS VFSQSFSPCG RYLAAGNNYG EIAVFSLSAA LSPDASERSQ KPILNFTAHD
     GPVFSLLSTE SHLLSAGNGE ISAWSWAELI KKSTKAAWTR KPNYETSLEI PEINAMIINP
     KDNNLIVGGG DNNIHIMDME TGTFKSVLKG HTDYIHCLSF KEREGEILSG GEDGAVRIWD
     SRTNRPVHCI EVFKYEECAR PQFGKWISCL ATDSDWMLCG GGPSLSLWHL RSMSPTSVFP
     LSGCQREAVS YQDLIMSVGE GPYVSHCLHG GTVKAQIPCS PSSLNTLALN LKNTEHRVMT
     VGGSSHQIDV FTNFSYRALS LSF