ID   THOPL_DICDI             Reviewed;         673 AA.
AC   Q54DD2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Thimet-like oligopeptidase;
DE            EC=3.4.24.-;
GN   ORFNames=DDB_G0292362;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the peptidase M3 family. {ECO:0000305}.
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DR   EMBL; AAFI02000189; EAL61305.1; -; Genomic_DNA.
DR   RefSeq; XP_629709.1; XM_629707.1.
DR   AlphaFoldDB; Q54DD2; -.
DR   SMR; Q54DD2; -.
DR   STRING; 44689.DDB0267057; -.
DR   MEROPS; M03.A07; -.
DR   PaxDb; Q54DD2; -.
DR   EnsemblProtists; EAL61305; EAL61305; DDB_G0292362.
DR   GeneID; 8628623; -.
DR   KEGG; ddi:DDB_G0292362; -.
DR   dictyBase; DDB_G0292362; -.
DR   eggNOG; KOG2089; Eukaryota.
DR   HOGENOM; CLU_001805_1_2_1; -.
DR   InParanoid; Q54DD2; -.
DR   OMA; KNFQSAM; -.
DR   PhylomeDB; Q54DD2; -.
DR   Reactome; R-DDI-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   PRO; PR:Q54DD2; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006518; P:peptide metabolic process; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR   Gene3D; 1.10.1370.10; -; 1.
DR   Gene3D; 1.20.1050.40; -; 1.
DR   Gene3D; 3.40.390.10; -; 1.
DR   InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR   InterPro; IPR024077; Neurolysin/TOP_dom2.
DR   InterPro; IPR024080; Neurolysin/TOP_N.
DR   InterPro; IPR045090; Pept_M3A_M3B.
DR   InterPro; IPR001567; Pept_M3A_M3B_dom.
DR   PANTHER; PTHR11804; PTHR11804; 1.
DR   Pfam; PF01432; Peptidase_M3; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome;
KW   Zinc.
FT   CHAIN           1..673
FT                   /note="Thimet-like oligopeptidase"
FT                   /id="PRO_0000327864"
FT   ACT_SITE        466
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         465
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         469
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         472
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   673 AA;  77499 MW;  C37E96082AD31AE5 CRC64;
     MEELIKLNFP KSVEEYKKQS EELMETYDKG LKEIIEIPKE KRTFANTFEA TDKLDVKFRN
     IESSLTFLAN ASTDEKVRDV ANEVEATLSK YSIATSMRED LYQAYVDCCK QNNDFKESDL
     SKEQLRYVSK TLEGFEKNGL QLPKEKREKL KEIMTKISDN SIKFSKNIAD DKTKLSFSME
     QLNGIPKETI ESFEKDESKP GNYFISLKYP DVIPTMKYCT VAETRKAVEF ANGSKCIKEN
     TPILEETCKL RFEAAQLLGF KDWASYKSNY LMVKSNDNIQ SFEERMRKLL TPHAVTEYDR
     LKQLKKKIYL EKGGNPETYD DHYYSYDNAF YNNVMLVQDY QVDNNLVKEY FPFEVVIKGI
     FNCYQELLNV KFTEVPAYQP WHEEVKMYSC VDTADSNKLL GHFYIDLFPR EGKYSHAAVW
     PLIPGYERAD GEKQYPVAAM LCNFTKPTPT TPSLLTHDEV VTFFHEFGHV MHNMSTKVHY
     SMFSGTSVER DFVECPSQLF EFWCWNKDVL VNKLSGHYKD HSKKLPTDLV ERMIAAKNLN
     VAIFYLRQIQ LALFDNAIHS GNPANFTNTA DLYHRIATDV ALNPNQKGTN PGCSFGHLLG
     GYDAQYYSYL YSECFSASIF EIFDKHGVMN KELGARLRNQ VLAVGGSQPS SETIENFLGS
     KPNEAAFLKT IGL