BRWD1_HUMAN
ID BRWD1_HUMAN Reviewed; 2320 AA.
AC Q9NSI6; C9JK25; O43721; Q5R2V0; Q5R2V1; Q6P2D1; Q8TCV3; Q96QG9; Q96QH0;
AC Q9NUK1;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 4.
DT 03-AUG-2022, entry version 202.
DE RecName: Full=Bromodomain and WD repeat-containing protein 1;
DE AltName: Full=WD repeat-containing protein 9;
GN Name=BRWD1; Synonyms=C21orf107, WDR9;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), TISSUE SPECIFICITY, AND VARIANT
RP PRO-1699.
RX PubMed=12359327; DOI=10.1016/s0167-4781(02)00421-9;
RA Ramos V.C., Vidal-Taboada J.M., Bergonon S., Egeo A., Fisher E.M.C.,
RA Scartezzini P., Oliva R.;
RT "Characterisation and expression analysis of the WDR9 gene, located in the
RT Down critical region-2 of the human chromosome 21.";
RL Biochim. Biophys. Acta 1577:377-383(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), AND VARIANTS PRO-1511 AND
RP PRO-1699.
RA Scott H.S., Barras C., Mittaz L., Michaud J., Guidi S., Scamuffa N.,
RA Antonarakis S.;
RT "Isolation and characterization of a new chromosome 21 gene, WDR9, with
RT different alternatively spliced transcripts and different protein forms.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND C), AND VARIANTS GLU-83 AND
RP PRO-1699.
RC TISSUE=Pancreas;
RA Obayashi I., Shibuya K., Minoshima S., Kudoh J., Shimizu N.;
RT "Isolation of WDR9 cDNA on human chromosome 21q22.3.";
RL Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10830953; DOI=10.1038/35012518;
RA Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S.,
RA Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M.,
RA Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A., Menzel U.,
RA Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A.,
RA Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J.,
RA Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K.,
RA Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G.,
RA Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J.,
RA Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S.,
RA Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K.,
RA Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.;
RT "The DNA sequence of human chromosome 21.";
RL Nature 405:311-319(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D).
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1560-1779, AND VARIANT PRO-1699.
RC TISSUE=Fetal heart;
RX PubMed=9480850; DOI=10.1006/bbrc.1998.8141;
RA Vidal-Taboada J.M., Bergonon S., Sanchez M., Lopez-Acedo C., Groet J.,
RA Nizetic D., Egeo A., Scartezzini P., Katsanis N., Fisher E.M.C.,
RA Delabar J.-M., Oliva R.;
RT "High resolution physical mapping and identification of transcribed
RT sequences in the Down syndrome region-2.";
RL Biochem. Biophys. Res. Commun. 243:572-578(1998).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1645-2320 (ISOFORM B), AND
RP VARIANT PRO-1699.
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1289; SER-1605; SER-1607;
RP SER-1904; SER-1905; SER-1907; SER-1910; SER-2018 AND SER-2020, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1475; SER-1605; SER-1607;
RP SER-2018 AND SER-2020, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-696; SER-710; THR-1477;
RP SER-1479; SER-1605; SER-1607; SER-1686; SER-2018 AND SER-2020,
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2214 (ISOFORM B), AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [11]
RP FUNCTION.
RX PubMed=21834987; DOI=10.1186/1741-7007-9-54;
RA Bai S.W., Herrera-Abreu M.T., Rohn J.L., Racine V., Tajadura V.,
RA Suryavanshi N., Bechtel S., Wiemann S., Baum B., Ridley A.J.;
RT "Identification and characterization of a set of conserved and new
RT regulators of cytoskeletal organisation, cell morphology and migration.";
RL BMC Biol. 9:54-54(2011).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-696; SER-701; THR-1477;
RP SER-1678; SER-1683; SER-1686; SER-2018 AND SER-2020, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [13]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-696; SER-1475 AND SER-1943,
RP AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [14]
RP X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS) OF 1310-1430.
RX PubMed=22464331; DOI=10.1016/j.cell.2012.02.013;
RA Filippakopoulos P., Picaud S., Mangos M., Keates T., Lambert J.P.,
RA Barsyte-Lovejoy D., Felletar I., Volkmer R., Muller S., Pawson T.,
RA Gingras A.C., Arrowsmith C.H., Knapp S.;
RT "Histone recognition and large-scale structural analysis of the human
RT bromodomain family.";
RL Cell 149:214-231(2012).
CC -!- FUNCTION: May be a transcriptional activator. May be involved in
CC chromatin remodeling (By similarity). Plays a role in the regulation of
CC cell morphology and cytoskeletal organization. Required in the control
CC of cell shape. {ECO:0000250, ECO:0000269|PubMed:21834987}.
CC -!- SUBUNIT: Interacts with SMARCA4. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9NSI6-4; P07550: ADRB2; NbExp=3; IntAct=EBI-10693038, EBI-491169;
CC Q9NSI6-4; P55212: CASP6; NbExp=3; IntAct=EBI-10693038, EBI-718729;
CC Q9NSI6-4; P06307: CCK; NbExp=3; IntAct=EBI-10693038, EBI-6624398;
CC Q9NSI6-4; P28329-3: CHAT; NbExp=3; IntAct=EBI-10693038, EBI-25837549;
CC Q9NSI6-4; P36544: CHRNA7; NbExp=3; IntAct=EBI-10693038, EBI-79333;
CC Q9NSI6-4; P99999: CYCS; NbExp=3; IntAct=EBI-10693038, EBI-446479;
CC Q9NSI6-4; P22607: FGFR3; NbExp=3; IntAct=EBI-10693038, EBI-348399;
CC Q9NSI6-4; Q53EP0-3: FNDC3B; NbExp=3; IntAct=EBI-10693038, EBI-10242151;
CC Q9NSI6-4; Q14957: GRIN2C; NbExp=3; IntAct=EBI-10693038, EBI-8285963;
CC Q9NSI6-4; Q8WUI4-6: HDAC7; NbExp=3; IntAct=EBI-10693038, EBI-12094670;
CC Q9NSI6-4; O00291: HIP1; NbExp=3; IntAct=EBI-10693038, EBI-473886;
CC Q9NSI6-4; P30519: HMOX2; NbExp=3; IntAct=EBI-10693038, EBI-712096;
CC Q9NSI6-4; P04792: HSPB1; NbExp=3; IntAct=EBI-10693038, EBI-352682;
CC Q9NSI6-4; O60333-2: KIF1B; NbExp=3; IntAct=EBI-10693038, EBI-10975473;
CC Q9NSI6-4; P13473-2: LAMP2; NbExp=3; IntAct=EBI-10693038, EBI-21591415;
CC Q9NSI6-4; Q16656-4: NRF1; NbExp=3; IntAct=EBI-10693038, EBI-11742836;
CC Q9NSI6-4; P61970: NUTF2; NbExp=3; IntAct=EBI-10693038, EBI-591778;
CC Q9NSI6-4; Q96CV9: OPTN; NbExp=3; IntAct=EBI-10693038, EBI-748974;
CC Q9NSI6-4; P62826: RAN; NbExp=3; IntAct=EBI-10693038, EBI-286642;
CC Q9NSI6-4; Q9Y3C5: RNF11; NbExp=3; IntAct=EBI-10693038, EBI-396669;
CC Q9NSI6-4; Q96GM5: SMARCD1; NbExp=3; IntAct=EBI-10693038, EBI-358489;
CC Q9NSI6-4; Q5JTV8: TOR1AIP1; NbExp=3; IntAct=EBI-10693038, EBI-2559665;
CC Q9NSI6-4; Q9UMX0: UBQLN1; NbExp=3; IntAct=EBI-10693038, EBI-741480;
CC Q9NSI6-4; O76024: WFS1; NbExp=3; IntAct=EBI-10693038, EBI-720609;
CC Q9NSI6-4; Q9Y649; NbExp=3; IntAct=EBI-10693038, EBI-25900580;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=A;
CC IsoId=Q9NSI6-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q9NSI6-2; Sequence=VSP_018526;
CC Name=C;
CC IsoId=Q9NSI6-3; Sequence=VSP_018527, VSP_018528;
CC Name=D;
CC IsoId=Q9NSI6-4; Sequence=VSP_044245, VSP_044246;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:12359327}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA10896.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AJ238214; CAC37033.2; -; mRNA.
DR EMBL; AJ292465; CAC44371.1; -; mRNA.
DR EMBL; AJ292466; CAC44372.1; -; mRNA.
DR EMBL; AB080586; BAD74071.1; -; mRNA.
DR EMBL; AB080587; BAD74072.1; -; mRNA.
DR EMBL; AF064861; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AF129408; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC064602; AAH64602.1; -; mRNA.
DR EMBL; AL163279; CAB90452.1; -; Genomic_DNA.
DR EMBL; AJ222636; CAA10896.1; ALT_FRAME; mRNA.
DR EMBL; AK002177; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS13662.1; -. [Q9NSI6-1]
DR CCDS; CCDS13663.1; -. [Q9NSI6-2]
DR CCDS; CCDS33557.1; -. [Q9NSI6-4]
DR RefSeq; NP_001007247.1; NM_001007246.2. [Q9NSI6-4]
DR RefSeq; NP_061836.2; NM_018963.4. [Q9NSI6-1]
DR RefSeq; NP_387505.1; NM_033656.3. [Q9NSI6-2]
DR PDB; 3Q2E; X-ray; 1.74 A; A=1310-1430.
DR PDBsum; 3Q2E; -.
DR AlphaFoldDB; Q9NSI6; -.
DR SMR; Q9NSI6; -.
DR BioGRID; 119828; 36.
DR IntAct; Q9NSI6; 46.
DR STRING; 9606.ENSP00000330753; -.
DR BindingDB; Q9NSI6; -.
DR ChEMBL; CHEMBL3351192; -.
DR GlyGen; Q9NSI6; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9NSI6; -.
DR PhosphoSitePlus; Q9NSI6; -.
DR BioMuta; BRWD1; -.
DR DMDM; 313104296; -.
DR EPD; Q9NSI6; -.
DR jPOST; Q9NSI6; -.
DR MassIVE; Q9NSI6; -.
DR MaxQB; Q9NSI6; -.
DR PaxDb; Q9NSI6; -.
DR PeptideAtlas; Q9NSI6; -.
DR PRIDE; Q9NSI6; -.
DR ProteomicsDB; 66890; -.
DR ProteomicsDB; 82555; -. [Q9NSI6-1]
DR ProteomicsDB; 82556; -. [Q9NSI6-2]
DR ProteomicsDB; 82557; -. [Q9NSI6-3]
DR Antibodypedia; 23299; 105 antibodies from 22 providers.
DR DNASU; 54014; -.
DR Ensembl; ENST00000333229.6; ENSP00000330753.2; ENSG00000185658.14. [Q9NSI6-1]
DR Ensembl; ENST00000341322.4; ENSP00000342106.4; ENSG00000185658.14. [Q9NSI6-4]
DR Ensembl; ENST00000342449.8; ENSP00000344333.3; ENSG00000185658.14. [Q9NSI6-2]
DR Ensembl; ENST00000380800.7; ENSP00000370178.3; ENSG00000185658.14. [Q9NSI6-3]
DR GeneID; 54014; -.
DR KEGG; hsa:54014; -.
DR MANE-Select; ENST00000342449.8; ENSP00000344333.3; NM_033656.4; NP_387505.1. [Q9NSI6-2]
DR UCSC; uc002yxk.3; human. [Q9NSI6-1]
DR CTD; 54014; -.
DR DisGeNET; 54014; -.
DR GeneCards; BRWD1; -.
DR HGNC; HGNC:12760; BRWD1.
DR HPA; ENSG00000185658; Low tissue specificity.
DR MIM; 617824; gene.
DR neXtProt; NX_Q9NSI6; -.
DR OpenTargets; ENSG00000185658; -.
DR PharmGKB; PA134906879; -.
DR VEuPathDB; HostDB:ENSG00000185658; -.
DR eggNOG; KOG0644; Eukaryota.
DR GeneTree; ENSGT00950000183107; -.
DR HOGENOM; CLU_001108_0_1_1; -.
DR InParanoid; Q9NSI6; -.
DR OMA; EGEWGMK; -.
DR OrthoDB; 240778at2759; -.
DR PhylomeDB; Q9NSI6; -.
DR TreeFam; TF324197; -.
DR PathwayCommons; Q9NSI6; -.
DR Reactome; R-HSA-1266695; Interleukin-7 signaling.
DR Reactome; R-HSA-3247509; Chromatin modifying enzymes.
DR SignaLink; Q9NSI6; -.
DR BioGRID-ORCS; 54014; 33 hits in 1131 CRISPR screens.
DR ChiTaRS; BRWD1; human.
DR EvolutionaryTrace; Q9NSI6; -.
DR GeneWiki; BRWD1; -.
DR GenomeRNAi; 54014; -.
DR Pharos; Q9NSI6; Tbio.
DR PRO; PR:Q9NSI6; -.
DR Proteomes; UP000005640; Chromosome 21.
DR RNAct; Q9NSI6; protein.
DR Bgee; ENSG00000185658; Expressed in cortical plate and 177 other tissues.
DR ExpressionAtlas; Q9NSI6; baseline and differential.
DR Genevisible; Q9NSI6; HS.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005730; C:nucleolus; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR GO; GO:0007010; P:cytoskeleton organization; IMP:UniProtKB.
DR GO; GO:0008360; P:regulation of cell shape; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.20.920.10; -; 2.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR001487; Bromodomain.
DR InterPro; IPR036427; Bromodomain-like_sf.
DR InterPro; IPR018359; Bromodomain_CS.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00439; Bromodomain; 2.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00503; BROMODOMAIN.
DR SMART; SM00297; BROMO; 2.
DR SMART; SM00320; WD40; 8.
DR SUPFAM; SSF47370; SSF47370; 2.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00633; BROMODOMAIN_1; 1.
DR PROSITE; PS50014; BROMODOMAIN_2; 2.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Alternative splicing; Bromodomain; Cytoplasm;
KW Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription;
KW Transcription regulation; WD repeat.
FT CHAIN 1..2320
FT /note="Bromodomain and WD repeat-containing protein 1"
FT /id="PRO_0000050888"
FT REPEAT 184..223
FT /note="WD 1"
FT REPEAT 226..265
FT /note="WD 2"
FT REPEAT 268..311
FT /note="WD 3"
FT REPEAT 322..365
FT /note="WD 4"
FT REPEAT 366..405
FT /note="WD 5"
FT REPEAT 424..463
FT /note="WD 6"
FT REPEAT 466..506
FT /note="WD 7"
FT REPEAT 514..553
FT /note="WD 8"
FT DOMAIN 1177..1247
FT /note="Bromo 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00035"
FT DOMAIN 1330..1400
FT /note="Bromo 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00035"
FT REGION 668..691
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 809..867
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 895..925
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1271..1304
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1434..1593
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1670..1805
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1817..1839
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1862..1899
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2014..2077
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2112..2184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 810..836
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 839..855
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1470..1507
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1521..1550
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1560..1584
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1673..1697
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1712..1732
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1751..1768
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1769..1784
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1865..1881
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2021..2043
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2044..2077
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2116..2137
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2138..2169
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 687
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT MOD_RES 696
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231,
FT ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163"
FT MOD_RES 701
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT MOD_RES 710
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT MOD_RES 1289
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 1475
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 1477
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:20068231,
FT ECO:0007744|PubMed:21406692"
FT MOD_RES 1479
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT MOD_RES 1605
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231"
FT MOD_RES 1607
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231"
FT MOD_RES 1678
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT MOD_RES 1683
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT MOD_RES 1686
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231,
FT ECO:0007744|PubMed:21406692"
FT MOD_RES 1755
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT MOD_RES 1756
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT MOD_RES 1786
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT MOD_RES 1788
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT MOD_RES 1793
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT MOD_RES 1820
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT MOD_RES 1867
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT MOD_RES 1871
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT MOD_RES 1904
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 1905
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 1907
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 1910
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 1943
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 1955
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT MOD_RES 2018
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231,
FT ECO:0007744|PubMed:21406692"
FT MOD_RES 2020
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231,
FT ECO:0007744|PubMed:21406692"
FT MOD_RES 2052
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT MOD_RES 2164
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT MOD_RES 2166
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q921C3"
FT VAR_SEQ 117..120
FT /note="DCRH -> GTLI (in isoform D)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_044245"
FT VAR_SEQ 121..2320
FT /note="Missing (in isoform D)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_044246"
FT VAR_SEQ 2191..2320
FT /note="EFVPRDREPNTKVRTCMHNQKDAVQMPSETLKAKMVPEKVPRRCATVAANKI
FT KIMSNLKETISGPENVWIRKSSRKLPHRNASAAAKKKLLNVYKEDDTTINSESEKELED
FT INRKMLFLRGFRSWKENAQ -> GKTFTANISKTVRRQRQSKRPRLSVDDNDWEDLDYA
FT KSKRVLRRSKIKTRNQGRRTVRYHDGDDDRSLENVLDFNGCTL (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12359327,
FT ECO:0000303|PubMed:14702039, ECO:0000303|Ref.2,
FT ECO:0000303|Ref.3"
FT /id="VSP_018526"
FT VAR_SEQ 2191..2285
FT /note="EFVPRDREPNTKVRTCMHNQKDAVQMPSETLKAKMVPEKVPRRCATVAANKI
FT KIMSNLKETISGPENVWIRKSSRKLPHRNASAAAKKKLLNVYK -> DKTFSPVYL
FT (in isoform C)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_018527"
FT VAR_SEQ 2286..2320
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_018528"
FT VARIANT 83
FT /note="Q -> E (in dbSNP:rs2056844)"
FT /evidence="ECO:0000269|Ref.3"
FT /id="VAR_026435"
FT VARIANT 1511
FT /note="S -> P (in dbSNP:rs2183573)"
FT /evidence="ECO:0000269|Ref.2"
FT /id="VAR_026436"
FT VARIANT 1699
FT /note="L -> P (in dbSNP:rs1041439)"
FT /evidence="ECO:0000269|PubMed:12359327,
FT ECO:0000269|PubMed:14702039, ECO:0000269|PubMed:9480850,
FT ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT /id="VAR_026437"
FT VARIANT 2156
FT /note="K -> R (in dbSNP:rs2234548)"
FT /id="VAR_057584"
FT CONFLICT 1560..1562
FT /note="SSS -> GTR (in Ref. 6; CAA10896)"
FT /evidence="ECO:0000305"
FT CONFLICT 1840
FT /note="M -> V (in Ref. 7; AK002177)"
FT /evidence="ECO:0000305"
FT CONFLICT 2134
FT /note="E -> G (in Ref. 7; AK002177)"
FT /evidence="ECO:0000305"
FT HELIX 1318..1330
FT /evidence="ECO:0007829|PDB:3Q2E"
FT HELIX 1333..1338
FT /evidence="ECO:0007829|PDB:3Q2E"
FT TURN 1344..1346
FT /evidence="ECO:0007829|PDB:3Q2E"
FT HELIX 1350..1353
FT /evidence="ECO:0007829|PDB:3Q2E"
FT HELIX 1360..1368
FT /evidence="ECO:0007829|PDB:3Q2E"
FT HELIX 1375..1392
FT /evidence="ECO:0007829|PDB:3Q2E"
FT HELIX 1399..1422
FT /evidence="ECO:0007829|PDB:3Q2E"
FT MOD_RES Q9NSI6-2:2214
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
SQ SEQUENCE 2320 AA; 262936 MW; 109714D79B9057E9 CRC64;
MAEPSSARRP VPLIESELYF LIARYLSAGP CRRAAQVLVQ ELEQYQLLPK RLDWEGNEHN
RSYEELVLSN KHVAPDHLLQ ICQRIGPMLD KEIPPSISRV TSLLGAGRQS LLRTAKDCRH
TVWKGSAFAA LHRGRPPEMP VNYGSPPNLV EIHRGKQLTG CSTFSTAFPG TMYQHIKMHR
RILGHLSAVY CVAFDRTGHR IFTGSDDCLV KIWSTHNGRL LSTLRGHSAE ISDMAVNYEN
TMIAAGSCDK IIRVWCLRTC APVAVLQGHT GSITSLQFSP MAKGSQRYMV STGADGTVCF
WQWDLESLKF SPRPLKFTEK PRPGVQMLCS SFSVGGMFLA TGSTDHVIRM YFLGFEAPEK
IAELESHTDK VDSIQFCNNG DRFLSGSRDG TARIWRFEQL EWRSILLDMA TRISGDLSSE
EERFMKPKVT MIAWNQNDSI VVTAVNDHVL KVWNSYTGQL LHNLMGHADE VFVLETHPFD
SRIMLSAGHD GSIFIWDITK GTKMKHYFNM IEGQGHGAVF DCKFSQDGQH FACTDSHGHL
LIFGFGCSKP YEKIPDQMFF HTDYRPLIRD SNNYVLDEQT QQAPHLMPPP FLVDVDGNPH
PTKYQRLVPG RENSADEHLI PQLGYVATSD GEVIEQIISL QTNDNDERSP ESSILDGMIR
QLQQQQDQRM GADQDTIPRG LSNGEETPRR GFRRLSLDIQ SPPNIGLRRS GQVEGVRQMH
QNAPRSQIAT ERDLQAWKRR VVVPEVPLGI FRKLEDFRLE KGEEERNLYI IGRKRKTLQL
SHKSDSVVLV SQSRQRTCRR KYPNYGRRNR SWRELSSGNE SSSSVRHETS CDQSEGSGSS
EEDEWRSDRK SESYSESSSD SSSRYSDWTA DAGINLQPPL RTSCRRRITR FCSSSEDEIS
TENLSPPKRR RKRKKENKPK KENLRRMTPA ELANMEHLYE FHPPVWITDT TLRKSPFVPQ
MGDEVIYFRQ GHEAYIEAVR RNNIYELNPN KEPWRKMDLR DQELVKIVGI RYEVGPPTLC
CLKLAFIDPA TGKLMDKSFS IRYHDMPDVI DFLVLRQFYD EARQRNWQSC DRFRSIIDDA
WWFGTVLSQE PYQPQYPDSH FQCYIVRWDN TEIEKLSPWD MEPIPDNVDP PEELGASISV
TTDELEKLLY KPQAGEWGQK SRDEECDRII SGIDQLLNLD IAAAFAGPVD LCTYPKYCTV
VAYPTDLYTI RMRLVNRFYR RLSALVWEVR YIEHNARTFN EPESVIARSA KKITDQLLKF
IKNQHCTNIS ELSNTSENDE QNAEDLDDSD LPKTSSGRRR VHDGKKSIRA TNYVESNWKK
QCKELVNLIF QCEDSEPFRQ PVDLVEYPDY RDIIDTPMDF GTVRETLDAG NYDSPLEFCK
DIRLIFSNAK AYTPNKRSKI YSMTLRLSAL FEEKMKKISS DFKIGQKFNE KLRRSQRFKQ
RQNCKGDSQP NKSIRNLKPK RLKSQTKIIP ELVGSPTQST SSRTAYLGTH KTSAGISSGV
TSGDSSDSAE SSERRKRNRP ITNGSTLSES EVEDSLATSL SSSASSSSEE SKESSRARES
SSRSGLSRSS NLRVTRTRAA QRKTGPVSLA NGCGRKATRK RVYLSDSDNN SLETGEILKA
RAGNNRKVLR KCAAVAANKI KLMSDVEENS SSESVCSGRK LPHRNASAVA RKKLLHNSED
EQSLKSEIEE EELKDENQLL PVSSSHTAQS NVDESENRDS ESESDLRVAR KNWHANGYKS
HTPAPSKTKF LKIESSEEDS KSHDSDHACN RTAGPSTSVQ KLKAESISEE ADSEPGRSGG
RKYNTFHKNA SFFKKTKILS DSEDSESEEQ DREDGKCHKM EMNPISGNLN CDPIAMSQCS
SDHGCETDLD SDDDKIEKPN NFMKDSASQD NGLSRKISRK RVCSSDSDSS LQVVKKSSKA
RTGLLRITRR CAATAANKIK LMSDVEDVSL ENVHTRSKNG RKKPLHLACT TAKKKLSDCE
GSVHCEVPSE QYACEGKPPD PDSEGSTKVL SQALNGDSDS EDMLNSEHKH RHTNIHKIDA
PSKRKSSSVT SSGEDSKSHI PGSETDRTFS SESTLAQKAT AENNFEVELN YGLRRWNGRR
LRTYGKAPFS KTKVIHDSQE TAEKEVKRKR SHPELENVKI SETTGNSKFR PDTSSKSSDL
GSVTESDIDC TDNTKTKRRK TKGKAKVVRK EFVPRDREPN TKVRTCMHNQ KDAVQMPSET
LKAKMVPEKV PRRCATVAAN KIKIMSNLKE TISGPENVWI RKSSRKLPHR NASAAAKKKL
LNVYKEDDTT INSESEKELE DINRKMLFLR GFRSWKENAQ
